STRATEGIES FOR THE ANALYSIS OF THIOLOXIDORDUCTASES

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Polish Society of Microbiologists

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VOLUME 56 , ISSUE 3 (April 2017) > List of articles

STRATEGIES FOR THE ANALYSIS OF THIOLOXIDORDUCTASES

Elżbieta Katarzyna Jagusztyn-Krynicka * / Anna Marta Banaś / Magdalena Joanna Grzeszczuk

Keywords : Dsb proteins, EcDsbA, disulfide bonds, protein structure, biochemical attributes

Citation Information : Postępy Mikrobiologii - Advancements of Microbiology. Volume 56, Issue 3, Pages 326-334, DOI: https://doi.org/10.21307/PM-2017.56.3.326

License : (CC BY-NC-ND 4.0)

Published Online: 22-May-2019

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ABSTRACT

Bacterial Dsb (disulfide bond) enzymes are involved in the oxidative folding of many proteins, through the formation of disulfide bonds between thiol groups of cysteine residues. This process is critical for the correct folding and structural stability of many secreted and membrane proteins. The rapidly expanding number of sequenced bacterial genomes has revealed the enormous diversity among bacterial Dsb systems. While the Escherichia coli oxidative protein folding has been studied in great details, the mechanism of the Dsb systems functioning in other bacteria are rather poorly understood. Herein, we present the current methodology, both in vivo and in vitro experimental techniques, which allow us to understand the functioning of the Dsb proteins and has broaden our knowledge in the field of biochemistry and microbiology of this posttranslational protein modification. Many bacterial virulence factors are extracytoplasmic Dsb-dependent proteins. Thus, this system plays an important role in bacterial pathogenesis and the proteins of the Dsb network represent possible targets for new drugs.

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