VOLUME 48 , ISSUE 3 (September 2016) - List of articles

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First Report of Longidorus kuiperi and Rotylenchus eximius from Coastal Sand Dunes in Crete, Greece

EMMANUEL A. TZORTZAKAKIS/ CAROLINA CANTALAPIEDRA-NAVARRETE/ ANTONIO ARCHIDONA-YUSTE/ JUAN E. PALOMARES-RIUS/ PABLO CASTILLO

Plant-parasitic nematode species have been reported on several occasions from coastal sand dunes, including Longidorus and Rotylenchus species (Vovlas et al., 2008; De Luca et al., 2009; Mateille et al., 2014). In April 2016, 10 soil samples of 3 to 4 kg from the rhizosphere of Tamarix smyrnensis with different vegetation around (viz. Elymus farctus, Lycium schweinfurthii, Crithmum maritimum, and Arthrocnemum sp.) were collected for diagnosis of plant-parasitic nematodes. The area of sampling wa(..)

DOI: 10.21307/jofnem-2017-019

First Report of the Spiral Nematode Rotylenchus incultus (Nematoda: Hoplolaimidae) from Cultivated Olive in Tunisia, with Additional Molecular Data on Rotylenchus eximius

ILHEM GUESMI-MZOUGHI/ ANTONIO ARCHIDONA-YUSTE/ CAROLINA CANTALAPIEDRA-NAVARRETE/ HAJER REGAIEG/ NAJET HORRIGUE-RAOUANI/ JUAN E. PALOMARES-RIUS/ PABLO CASTILLO

Spiral nematode species of the genus Rotylenchus have been reported on olive (Olea europaea L.) in several Mediterranean countries (Castillo et al., 2010; Ali et al., 2014). Nematological surveys for plant-parasitic nematodes on olive trees were carried out in Tunisia between 2013 and 2014, and two nematode species of Rotylenchus were collected from the rhizosphere of olive cv. Chemlali in several localities of Tunisia (Tables 1,2). Twenty-two soil samples of 3 to 4 kg were collected with a shov(..)

DOI: 10.21307/jofnem-2017-020

First Report of Heterorhabditis amazonensis from Venezuela and Characterization of Three Populations

NAIYULIN MORALES/ PATRICIA MORALES-MONTERO/ VLADIMIR PUZA/ ERNESTO SAN-BLAS

During a survey in western Venezuela in 2011, three new populations of Heterorhabditis amazonensis (LPV081, LPV156, and LPV498) were isolated. Some differences were found in terms of morphometry compared with the original description; however, the distance from the anterior end to the excretory pore is the most variable character; significantly shorter in all infective juveniles and in other developmental stages depending on the population. According to a Principal Component Analysis, LPV498 pos(..)

DOI: 10.21307/jofnem-2017-021

Steinernema biddulphi n. sp., a New Entomopathogenic Nematode (Nematoda: Steinernematidae) from South Africa

HARUN CIMEN/ VLADIMI´R PU°zA/ JIRI NERMUT/ JUSTIN HATTING/ TSHIMA RAMAKUWELA/ SELCUK HAZIR

A new species of entomopathogenic nematode (EPN), Steinernema biddulphi n. sp., was isolated from a maize field in Senekal, Free State Province of South Africa. Morphological and molecular studies indicated the distinctness of S. biddulphi n. sp. from other Steinernema species. Steinernema biddulphi n. sp. is characterized IJs with average body length of 663 mm (606–778 mm), lateral fields with six ridges in mid-body region forming the formula 2,6,2. Excretory pore located anterior to mid-pharyn(..)

DOI: 10.21307/jofnem-2017-022

Coronostoma claireae n. sp. (Nematoda: Rhabditida: Oxyuridomorpha: Coronostomatidae) from the Indigenous Milliped Narceus gordanus (Chamberlain, 1943) (Diplopoda: Spirobolida) in Ocala National Forest, Florida

GARY PHILLIPS/ ERNEST C. BERNARD/ ROBERT J. PIVAR/ JOHN K. MOULTON/ ROWLAND M. SHELLEY

Twenty-four individuals of Narceus gordanus (Diplopoda: Spirobolidae) were collected in Ocala National Forest, FL, between November 2013 and July 2014. Each specimen was dissected to extract the intestine, which was removed and examined for parasitic nematodes. Coronostoma claireae n. sp. was collected from the hindgut and midgut of 10 specimens, and its morphology was examined with brightfield, differential interference contrast, phase contrast, and scanning electron microscopy. This species is(..)

DOI: 10.21307/jofnem-2017-023

Curative Control of the Peachtree Borer Using Entomopathogenic Nematodes

DAVID I. SHAPIRO-ILAN/ TED E. COTTRELL/ RUSSELL F. MIZELL/ DAN L. HORTON

The peachtree borer, Synanthedon exitiosa (Say 1823), is a major pest of stone fruit trees in North America. Current management relies upon preventative control using broad-spectrum chemical insecticides, primarily chlorpyrifos, applied in the late summer or early fall. However, due to missed applications, poor application timing, or other factors, high levels of S. exitiosa infestation may still occur and persist through the following spring. Curative treatments applied in the spring to establi(..)

DOI: 10.21307/jofnem-2017-024

Effect of Application Timing of Oxamyl in Nonbearing Raspberry for Pratylenchus penetrans Management

INGA A. ZASADA/ THOMAS W. WALTERS

In 2012, theWashington raspberry (Rubus idaeus) industry received a special local needs (SLN) 24(c) label to apply Vydate L (active ingredient oxamyl) to nonbearing raspberry for the management of Pratylenchus penetrans. This is a new use pattern of this nematicide for raspberry growers; therefore, research was conducted to identify the optimum spring application timing of oxamyl for the suppression of P. penetrans. Three on-farm trials in each of 2012 and 2013 were established in Washington in (..)

DOI: 10.21307/jofnem-2017-025

Evaluation of Steam and Soil Solarization for Meloidogyne arenaria Control in Florida Floriculture Crops

NANCY KOKALIS-BURELLE/ ERIN N. ROSSKOPF/ DAVID M. BUTLER/ STEVEN A. FENNIMORE/ JOHN HOLZINGER

Steam and soil solarization were investigated for control of the root-knot nematode Meloidogyne arenaria in 2 yr of field trials on a commercial flower farm in Florida. The objective was to determine if preplant steam treatments in combination with solarization, or solarization alone effectively controlled nematodes compared to methyl bromide (MeBr). Trials were conducted in a field with naturally occurring populations of M. arenaria. Treatments were solarization alone, steam treatment after sol(..)

DOI: 10.21307/jofnem-2017-026

Mitochondrial Haplotype-based Identification of Root-knot Nematodes (Meloidogyne spp.) on Cut Foliage Crops in Florida

RICHARD BAIDOO/ SOUMI JOSEPH/ TESFAMARIAM M. MENGISTU/ JANETE A. BRITO/ ROBERT MCSORLEY/ ROBERT H. STAMPS/ WILLIAM T. CROW

Florida accounts for more than 75% of the national cut foliage production. Unfortunately, root-knot nematodes (RKN) (Meloidogyne spp.) are a serious problem on these crops, rendering many farms unproductive. Currently, information on the Meloidogyne spp. occurring on most commonly cultivated cut foliage crops in Florida, and tools for their rapid identification are lacking. The objectives of this study were to (i) identify specific RKN infecting common ornamental cut foliage crops in Florida and(..)

DOI: 10.21307/jofnem-2017-027

Morphological and Molecular Characterization of Gracilacus wuae n. sp. (Nematoda: Criconematoidea) Associated with Cow Parsnip (Heracleum maximum) in Ontario, Canada

QING YU/ WEIMIN YE/ TOM POWERS

Gracilacus wuae n. sp. from soil associated with cow parsnip in Ontario, Canada is described and illustrated. Morphologically, females have a long stylet ranging from 80 to 93 mmlong, the lip region not offset from the body contour, without lateral lips but with large and flat submedian lobes, the mouth opening slit-like elongated laterally and surrounded by lateral flaps, the excretory pore is anterior to the knobs of the stylet; males without stylet and the pharynx degenerated. The fourth-stag(..)

DOI: 10.21307/jofnem-2017-028

Discopersicus n. gen., a New Member of the Family Tylenchidae Örley, 1880 with Detailed SEM Study on Two Known Species of the Genus Discotylenchus Siddiqi, 1980 (Nematoda; Tylenchidae) from Iran

ALI YAGHOUBI/ EBRAHIM POURJAM/ SERGIO A LVAREZ-ORTEGA/ GRACIA LIE´BANAS/ MOHAMMAD REZA ATIGHI/ MAJID PEDRAM

Discopersicus iranicus n. gen., n. comb., previously described from Iran as a new species under the genus Discotylenchus, is illustrated using light microscope and scanning electron microscope (SEM) observations and further studied using molecular characters. SEM studies revealed the newly proposed genus has oblique amphidial apertures on the lateral sides of the lip region. SEM images are also provided for two species of Discotylenchus, namely D. discretus and D. brevicaudatus, as the first SEM(..)

DOI: 10.21307/jofnem-2017-029

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