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Journal of Nematology

Society of Nematologists

Subject: Life Sciences


ISSN: 0022-300X
eISSN: 2640-396X



Enhanced entomopathogenic nematode yield and fitness via addition of pulverized insect powder to solid media
Nematotoxic coumarins from Angelica pubescens Maxim. f. biserrata Shan et Yuan roots and their physiological effects on Bursaphelenchus xylophilus
Broad-based root-knot nematode resistance identified in cowpea gene-pool two
Description of Pseudacrobeles (Pseudacrobeles) curvatus sp. n. (Cephalobidae: Rhabditida) in South Korea

VOLUME 50 , ISSUE 4 (December 2018) - List of articles

Biological characterization of the entomopathogenic nematode, Steinernema innovationi: a South African isolate

Tshimangadzo Ramakuwela/ Justin Hatting/ Mark D. Laing/ Nicolene Thiebaut/ Selcuk Hazir

Entomopathogenic nematode species perform differently under different environmental conditions; therefore, the authors investigated the biological and environmental characteristics that could optimize performance of Steinernema innovationi. The authors studied the effect of temperature on infectivity and reproduction, the foraging behavior and host range. Thermal activity was optimal between 22 and 25°C. Highest infective juvenile (IJ) yields in last instar Galleria mellonella were observed at 2(..)

DOI: 10.21307/jofnem-2018-049

Five life stage-specific transcriptome assemblies for the reniform nematode, Rotylenchulus reniformis Linford & Oliveira

Kurt C. Showmaker/ William S. Sanders/ Sebastian Eves-van den Akker/ Brigitte E. Martin/ Franklin E. Callahan/ Daniel G. Peterson/ Martin J. Wubben

The reniform nematode (Rotylenchulus reniformis Linford and Oliveira) is a semi-endoparasitic nematode that is a pathogen of numerous major crops such as cotton and soybean. Here, the authors present transcriptome assemblies of the egg, second-stage juvenile (J2), J3, vermiform adult, and sedentary female life stages of this important plant pathogen.

DOI: 10.21307/jofnem-2018-053

Characterization of Juvenile Stages of Bursaphelenchus crenati Rühm, 1956 (Nematoda: Aphelenchoidoidea)

Alexander Y. Ryss/ Kristina S. Polyanina

In this study juvenile stages of Bursaphelenchus crenati (Sexdentati group) were distinguished based mainly on the genital primordium structure. Dauer stage juveniles were sampled under elytra of Hylesinus crenatus in galleries in bark of the wilted Fraxinus excelsior in the Voronezh region of Russia and were multiplied in laboratory cultures on the fungus Botrytis cinerea. Individual development included five stages (J1-J4 and adults) separated by molts. The first molt J1 to J2 occurred inside (..)

DOI: 10.21307/jofnem-2018-042

First report of Bursaphelenchus antoniae from Pinus strobus in the U.S.

Lynn K. Carta/ R. L. Wick

Juvenile, female and male nematodes were discovered in wood chips of white pine Pinus strobus from Ashley Falls, MA. Initial observations suggested these nematodes might be PWN, but closer morphological and molecular characterization proved otherwise. Comparison of measured features with those in the literature indicated this nematode population had some unique characteristics. The specimens were identified as Bursaphelenchus antoniae Penas et al., 2006 based on 18S rDNA molecular sequence vs on(..)

DOI: 10.21307/jofnem-2018-052

Two nematodes (Nematoda: Diplogastridae, Rhabditidae) from the invasive millipede Chamberlinius hualienensis Wang, 1956 (Diplopoda, Paradoxosomatidae) on Hachijojima Island in Japan

L. K. Carta/ W. K. Thomas/ V. B. Meyer-Rochow

Millipedes may cause unexpected damage when they are introduced to new locations, becoming invaders that leave behind their old parasites and predators. Therefore, it was interesting to find numerous rhabditid nematodes within the gut of the invasive phytophagous millipede Chamberlinius hualienensis Wang, 1956 (Diplopoda, Paradoxosomatidae) from Hachijojima (Japan) in November, 2014. This millipede originated in Taiwan but was discovered in Japan in 1986. The nematodes were identified as juvenil(..)

DOI: 10.21307/jofnem-2018-048

A novel in vitro chemotaxis bioassay to assess the response of Meloidogyne incognita towards various test compounds

Tagginahalli N. Shivakumara/ Tushar K. Dutta/ Uma Rao

Plant-parasitic, root-knot nematodes (Meloidogyne spp.) are a serious problem in agri- and horticultural crops worldwide. Understanding their complex host recognition process is essential for devising efficient and environmental-friendly management tactics. In this study, the authors report a new, simple, inexpensive, efficient, and quantitative method to analyze the chemotaxis of M. incognita second-stage juveniles (J2s) using a combination of pluronic gel and agar in a petri dish. The authors (..)

DOI: 10.21307/jofnem-2018-047

Enhanced entomopathogenic nematode yield and fitness via addition of pulverized insect powder to solid media

Shiyu Zhen/ Yang Li/ Yanli Hou/ Xinghui Gu/ Limeng Zhang/ Weibin Ruan/ David Shapiro-Ilan

Beneficial nematodes are used as biological control agents. Low-cost mass production of entomopathogenic nematodes (EPNs) is an important prerequisite toward their successful commercialization. EPNs can be grown via in vivo methods or in sold or liquid fermentation. For solid and liquid approaches, media optimization is paramount to maximizing EPN yield and quality. In solid media, the authors investigated the effects of incorporating pulverized insect powder from larvae of three insects (Galler(..)

DOI: 10.21307/jofnem-2018-050

Effector gene vap1 based DGGE fingerprinting to assess variation within and among Heterodera schachtii populations

Rasha Haj Nuaima/ Johannes Roeb/ Johannes Hallmann/ Matthias Daub/ Sandra Otte/ Holger Heuer

Populations of beet cyst nematodes Heterodera schachtii vary in aggressiveness and virulence toward sugar beet varieties, but also in traits like host range, or decline rate in the field. Diversity of their essential pathogenicity gene vap1 is shaped by diversifying selection and gene flow. The authors developed a technique to study inter-population variation and intra-population diversity and dynamics of H. schachtii based on the gene vap1. Degenerate primers were designed to amplify, clone, an(..)

DOI: 10.21307/jofnem-2018-055

First Report of the Ring Nematode Mesocriconema nebraskense from a Corn Field in North Dakota

Guiping Yan/ Addison Plaisance/ Danqiong Huang/ Richard Baidoo/ Joel K. Ransom/ Zafar A. Handoo

DOI: 10.21307/jofnem-2018-043

Improved 18S small subunit rDNA primers for problematic nematode amplification

L. K. Carta/ S. Li

The 18S small subunit (SSU) ribosomal DNA sequence is one of the most useful molecular loci for identification and phylogeny reconstruction of agriculturally important nematodes. Various pairs of universal primers have been developed in the past to amplify short and long nematode sequences. However, certain nematode taxa were not readily amplified and/or sequenced with the existing primer tools. Frequently, the center region of a roughly 1,000 nucleotide segment would be lost. Therefore new prim(..)

DOI: 10.21307/jofnem-2018-051

First Report of the Root-Knot Nematode (Meloidogyne javanica) Infecting Hops (Humulus lupulus) in Florida, USA

J. A. Brito/ S. A. Subbotin/ J. Desaeger/ F. Achinelly/ S. Qiu

DOI: 10.21307/jofnem-2018-040

Broad-based root-knot nematode resistance identified in cowpea gene-pool two

Arsenio D. Ndeve/ William C. Matthews/ Jansen R. P. Santos/ Bao Lam Huynh/ Philip A. Roberts

Cowpea (Vigna unguiculata L. Walp) is an affordable source of protein and strategic legume crop for food security in Africa and other developing regions; however, damage from infection by root-knot nematodes (RKN) suppresses cowpea yield. The deployment through breeding of resistance gene Rk in cowpea cultivars has provided protection to cowpea growers worldwide for many years. However, occurrence of more aggressive nematode isolates threatens the effectiveness of this monogenic resistance. A co(..)

DOI: 10.21307/jofnem-2018-046

Nematotoxic coumarins from Angelica pubescens Maxim. f. biserrata Shan et Yuan roots and their physiological effects on Bursaphelenchus xylophilus

Qun-Qun Guo/ Gui-Cai Du/ Yong-Xing Li/ Chen-Yan Liang/ Chao Wang/ Ya-Nan Zhang/ Rong-Gui Li

The ethanol extracts from the roots of Angelica pubescens Maxim. f. biserrata Shan et Yuan was toxic against the pine wood nematode Bursaphelenchus xylophilus. The ethyl acetate-soluble fraction derived from this extract increased its potency with a mortality of 95.25% in 72 hr at 1.0 mg/mL. Four nematotoxic coumarins were obtained from the ethyl acetate extract by bioassay-guided isolation. These were identified as osthole 1, columbianadin 2, bergapten 3 and xanthotoxin 4 by mass and nuclear ma(..)

DOI: 10.21307/jofnem-2018-045

Effects of vermicompost water extract prepared from bamboo and kudzu against Meloidogyne incognita and Rotylenchulus reniformis

Xiaodong You/ Motoaki Tojo/ Shelby Ching/ Koon-Hui Wang

A series of experiments in laboratory, greenhouse, and field were conducted to compare the nematode suppressive effect of vermicompost tea (VCT) prepared from vermicompost with moso-bamboo (Phyllostachys edulis (Carrière) J. Houz.) and kudzu (Pueraria lobata (Willd) Ohwi) as feed stock (weed VCT) to that prepared from vegetable food waste (vegetable VCT) against Meloidogyne incognita and Rotylenchulus reniformis. Two laboratory trials were conducted by incubating eggs of M. incognita and R. reni(..)

DOI: 10.21307/jofnem-2018-054

First report of Pratylenchus vulnus associated with apple in Tunisia

Noura Chihani-Hammas/ Lobna Hajji-Hedfi/ Hajer Regaieg/ Asma Larayedh/ Ahmed Badiss/ Yu Qing/ Horrigue-Raouani Najet

The root-lesion nematode of the genus Pratylenchus Filipjev (1936) has a worldwide distribution and cause severe production constraints on numerous important crops. In 2013-14, during a survey of the apple nurseries and orchards in center of Tunisia (Kairouan, Zaghouan, Monastir and Kasserine), 70 different roots and soil samples were collected. The populations of root-lesion nematode were identified on the basis of their morphological and morphometric characters, and by molecular methods. Micro(..)

DOI: 10.21307/jofnem-2018-056

Samplings of Millipedes in Japan and Scarab Beetles in Hong Kong result in five new Species of Pristionchus (Nematoda: Diplogastridae)

Natsumi Kanzaki/ Matthias Herrmann/ Kohta Yoshida/ Christian Weiler/ Christian Rödelsperger/ Ralf J. Sommer

The authors describe five new species of Pristionchus from Japan and Hongkong. Scarab beetle samplings in Hongkong identified P. hongkongensis sp. n. and P. neolucani sp. n., representing the first beetle-associated Pristionchus species from China. Surprisingly, samplings of millipedes in Japan revealed a previously unknown association of Pristionchus nematodes with these arthropods. Specifically, the authors found three previously known Pristionchus species, P. arcanus, P. entomophagus, and P. (..)

DOI: 10.21307/jofnem-2018-044

Description of Gracilacus paralatescens n. sp. (Nematoda:Paratylenchinae) found from the rhizosphere of Bamboo in Zhejiang, China

Munawar Maria/ Ruihang Cai/ Weimin Ye/ Thomas O. Powers/ Jingwu Zheng

Gracilacus paralatescens n. sp., isolated in Anji County, Zhejiang Province, China from the rhizospheric soil of bamboo. The new species can be characterized by the female lateral field with three incisures, stylet 71.5 to 78.8 µm long, lip region truncated, excretory pore located anterior to basal knobs. Vulval lips non-protruding and without vulval flap, spermatheca large, elongated squarish shaped filled with sperms. Tail slender, relatively straight having wedge shape terminus. The spicule s(..)

DOI: 10.21307/jofnem-2018-041

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