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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology


ISSN: 1733-1331
eISSN: 2544-4646



Differentially Marked IncP-1β R751 Plasmids for Cloning via Recombineering and Conjugation
Colistin Resistance in Enterobacterales Strains – A Current View
Antibiotic Resistance Among Uropathogenic Escherichia coli

VOLUME 65 , ISSUE 1 (March 2016) - List of articles

The Functions of Effector Proteins in Yersinia Virulence

Linglin Zhang/ Meng Mei/ Chan Yu/ Wenwen Shen/ Lixin Ma/ Jiewang He/ Li Yi

Yersinia species are bacterial pathogens that can cause plague and intestinal diseases after invading into human cells through the Three Secre­tion System (TTSS). The effect of pathogenesis is mediated by Yersinia outer proteins (Yop) and manifested as down-regulation of the cytokine genes expression by inhibiting nuclear factor-κ-gene binding (NF-κB) and mitogen-activated protein kinase (MAPK) pathways. In addition, its pathogenesis can also manipulate the disorder of host innate immune system (..)

DOI: 10.5604/17331331.1197324

Published Online: 15-March-2016

Recovery of Gram-Positive Cocci and Candida albicans from Peroxygen/Silver-Based Disinfectants

Mostafa E. Eissa/ Ahmed S. Nouby

Neutralization method evaluation is an important first step in a disinfectant validation study program. It is also crucial in assessment of the efficiency of microbial recovery media in the presence of a residual biocidal agent. In the present study, four commercially available peroxygen/silver-based disinfectant formulae – intended to be used in a pharmaceutical facility sanitization program – were tested at two dilutions against three Gram-positive cocci and one yeast; Staphylococcus aureus (A(..)

DOI: 10.5604/17331331.1197271

Published Online: 15-March-2016

Effectiveness of Antipseudomonal Antibiotics and Mechanisms of Multidrug Resistance in Pseudomonas aeruginosa

Mohamed E. El Zowalaty/ Béla Gyetvai

Pseudomonas aeruginosa is a leading human pathogen that causes serious infections at various tissues and organs leading to life threaten­ing health problems and possible deadly outcomes. Resistance patterns vary widely whether it is from hospitals or community acquired infections. Reporting resistance profiles to a certain antibiotics provide valuable information in a given setting, but may be extrapolated outside the sampling location. In the present study, P. aeruginosa isolates were screened (..)

DOI: 10.5604/17331331.1197272

Published Online: 15-March-2016

Streptococcus anginosus (milleri) Group Strains Isolated in Poland (1996–2012) and their Antibiotic Resistance Patterns

Katarzyna Obszańska/ Izabella Kern-Zdanowicz/ Aleksandra Kozińska/ Katarzyna Machura/ Elżbieta Stefaniuk/ Waleria Hryniewicz/ Izabela Sitkiewicz

Streptococcus anginosus, Streptococcus intermedius and Streptococcus constellatus form a group of related streptococcal species, namely the Streptococcus Anginosus Group (SAG). The group, previously called “milleri” had been rarely described until 1980/1990 as source of infections. Nowadays SAG bacteria are often described as pathogens causing predominantly purulent infections. The number of infec­tions is highly underestimated, as SAG strains are often classified in the microbiology laboratory (..)

DOI: 10.5604/17331331.1197323

Published Online: 15-March-2016

Characterization and Optimization of Biosynthesis of Bioactive Secondary Metabolites Produced by Streptomyces sp. 8812

Aleksandra Rajnisz/ Adam Guśpiel/ Magdalena Postek/ Joanna Ziemska/ Anna Laskowska/ Daniel Rabczenko/ Jolanta Solecka

The nutritional requirements and environmental conditions for a submerged culture of Streptomyces sp. 8812 were determined. Batch and fed-batch Streptomyces sp. 8812 fermentations were conducted to obtain high activity of secondary metabolites. In the study several factors were examined for their influence on the biosynthesis of the active metabolites-7-hydroxy-6-oxo-2,3,4,6-tetrahydroisoquinoline-3-carboxyl acid (C10H9NO4) and N-acetyl-3,4-dihydroxy-l-phenylalanine (C11H13NO5): changes in mediu(..)

DOI: 10.5604/17331331.1197275

Published Online: 15-March-2016

Isolation and Characterization of α-Endosulfan Degrading Bacteria from the Microflora of Cockroaches

Murat Ozdal/ Ozlem Gur Ozdal/ Omer Faruk Algur

Extensive applications of organochlorine pesticides like endosulfan have led to the contamination of soil and environments. Five different bacteria were isolated from cockroaches living in pesticide contaminated environments. According to morphological, physiological, bio­chemical properties, and total cellular fatty acid profile by Fatty Acid Methyl Esters (FAMEs), the isolates were identified as Pseudomonas aeruginosa G1, Stenotrophomonas maltophilia G2, Bacillus atrophaeus G3, Citrobacter amo(..)

DOI: 10.5604/17331331.1197325

Published Online: 15-March-2016

Bacterial Diversity and Composition in Oylat Cave (Turkey) with Combined Sanger/Pyrosequencing Approach

Yasemin Gulecal-Pektas

The microbiology of caves is an important topic for better understanding subsurface biosphere diversity. The diversity and taxonomic composition of bacterial communities associated with cave walls of the Oylat Cave was studied first time by molecular cloning basedon Sanger/pyrosequencing approach. Results showed an average of 1,822 operational taxonomic units per sample. Clones analyzed from Oylat Cave were found to belong to 10 common phyla within the domain Bacteria. Proteobacteria dominated t(..)

DOI: 10.5604/17331331.1197277

Published Online: 15-March-2016

Biochemical and Molecular Characterization of Carotenogenic Flavobacterial Isolates from Marine Waters

Rama Sowmya/ Nakkarike M. Sachindra

Carotenoids are known to possess immense nutraceutical properties and microorganisms are continuously being explored as natural source for production of carotenoids. In this study, pigmented bacteria belonging to Flavobacteriaceae family were isolated using kanamycin-containing marine agar and identified using the molecular techniques and their phenotypic characteristics were studied along with their potential to produce carotenoids. Analysis of random amplification of polymorphic DNA (RAPD) ban(..)

DOI: 10.5604/17331331.1197278

Published Online: 15-March-2016

Serotype-Specific Pneumococcal Status prior to PCV 13 Administration in Children and Adolescents with Inflammatory Bowel Disease

Aleksandra Banaszkiewicz/ Brygida Targońska/ Kinga Kowalska-Duplaga/ Katarzyna Karolewska-Bochenek/ Agnieszka Sieczkowska/ Agnieszka Gawrońska/ Urszula Grzybowska-Chlebowczyk/ Elżbieta Krzesiek/ Izabella Łazowska-Przeorek/ Maria Kotowska/ Edyta Sienkiewicz/ Jarosław Walkowiak/ Hanna Gregorek/ Andrzej Radzikowski/ Piotr Albrecht

The aim of this study was to evaluate the serotype-specific pneumococcal status of children and adolescents with inflammatory bowel disease (IBD) who were naïve to pneumococcal vaccination before administering the 13-valent pneumococcal conjugate vaccine (PCV 13). This was an open, prospective study on children and adolescents aged 5–18 years who had IBD and were naïve to pneumococcal vac­cination. A single dose of PCV 13 was administered to each patient. The geometric mean concentrations (GMCs)(..)

DOI: 10.5604/17331331.1197279

Published Online: 15-March-2016

Prevalence of Urogenital Mycoplasmas Among Men with NGU in Upper Silesia, Poland. Preliminary Study

Alicja Ekiel/ Małgorzata Aptekorz/ Piotr Kłuciński/ Dominika Smolec/ Barbara Wiechuła/ Jarosław Jóźwiak/ Gayane Martirosian

The prevalence of urogenital mycoplasmas in men with NGU in Upper Silesia (Poland) was studied. Mycoplasmas were detected in 36.7% men (Ureaplasma parvum and Mycoplasma genitalium were found in 30% and 16.7% respectively). Urealyticum urealyticum was not detected. We suggest including M. genitalium in the diagnostic scheme for nongonococcal urethritis (NGU).

DOI: 10.5604/17331331.1197326

Published Online: 15-March-2016

Assessment of the Microbiological Status of Probiotic Products

Anna Zawistowska-Rojek/ Tomasz Zaręba/ Agnieszka Mrówka/ Stefan Tyski

The aim of this study was to perform the microbiological analysis of quality of 25 probiotic products, available on the Polish market. Analysis of bacterial viability in probiotic products showed that not all of these preparations possess a suitable number of bacteria. Moreover, some of the tested probiotic products contained bacterial strains other than those declared by the manufacturer. All tested strains recovered from probiotic products were found to be resistant to metronidazole and suscep(..)

DOI: 10.5604/17331331.1197281

Published Online: 15-March-2016

Antimicrobial Activity of Penicillin G and N-acetylcystein on Planktonic and Sessile Cells of Streptococcus suis

Ivette Espinosa/ Michel Báez/ Evelyn Lobo/ Siomara Martínez/ Marcelo Gottschalk

The aim of this study was to investigate the capacity of Streptococcus suis strains to form biofilms and to evaluate the antimicrobial activity of Penicillin G and N-acetylcystein (NAC) on both S. suis sessile and planktonic forms. Only non-typeable isolates of S. suis were correlated with a greater biofilm formation capacity. The MCI of Penicillin G and NAC required for inhibiting biofilm growth were higher than the required concentration for inhibiting planktonic growth. The combinations of NA(..)

DOI: 10.5604/17331331.1197282

Published Online: 15-March-2016

Usefulness of CHROMagar Candida Medium, Biochemical Methods – API ID32C and VITEK 2 Compact and Two MALDI-TOF MS Systems for Candida spp. Identification

Elżbieta Stefaniuk/ Anna Baraniak/ Monika Fortuna/ Waleria Hryniewicz

This study was conducted to compare of the yeasts identification results obtained with two new systems using the MALDI-TOF MS technique with the ones obtained using the routine identification methods of Candida spp. in clinical microbiology laboratories. All 124 Candida spp. isolates were recovered from the routine examination of clinical specimens in microbiological laboratories and collected in the Centre of Quality Control in Microbiology in Warsaw (Poland). Our findings confirm the high agre(..)

DOI: 10.5604/17331331.1197283

Published Online: 15-March-2016

Enterobacter asburiae KUNi5, a Nickel Resistant Bacterium for Possible Bioremediation of Nickel Contaminated Sites

Anirudha Paul/ Samir Kumar Mukherjee

Nickel resistant bacterial strain Enterobacter asburiae KUNi5 was isolated and showed resistance up to 15 mM and could remove Ni opti­mally better at 37°C and pH 7. Maximum removal was found at initial concentration of 0.5 to 2 mM, however, growth and Ni removal were affected by other heavy metals. Major amount of the metal was accumulated in the membrane fractions and certain negatively charged groups were found responsible for Ni binding. KUNi5 could also produce 1-aminocyclopropane-1-carboxyl(..)

DOI: 10.5604/17331331.1197284

Published Online: 15-March-2016

IgG Avidity: an Important Serologic Marker for the Diagnosis of Tick-Borne Encephalitis Virus Infection

Tatjana Vilibic-Cavlek/ Ljubo Barbic/ Vladimir Stevanovic/ Goranka Petrovic/ Gordana Mlinaric-Galinovic

A total of 52 serum samples from patients with symptoms suggestive of tick-borne encephalitis virus (TBEV) infection and positive IgM and/or IgG antibodies were tested for IgG avidity. Acute/recent TBEV infection was confirmed by low/borderline avidity index (AI) in 94.8% IgM positive/IgG positive samples, while in 5.2% high AI was found indicating persisting IgM antibodies. Majority of IgM negative/IgG positive samples (78.6%) showed high AI consistent with past TBEV infection. However, in 21.3(..)

DOI: 10.5604/17331331.1197285

Published Online: 15-March-2016

Etiologic Agents and Antifungal Susceptibility of Oral Candidosis from Romanian patients with HIV-infection or type 1 diabetes mellitus

Bogdan Minea/ Valentin Nastasa/ Anna Kolecka/ Magdalena Mares/ Narcisa Marangoci/ Irina Rosca/ Mariana Pinteala/ Monica Hancianu/ Mihai Mares

This is the first Romanian investigation of oral candidosis in patients suffering of HIV-infection or type 1 diabetes mellitus (T1DM).Candida albicans was the dominant species in both types of isolates: n = 14 (46.7%) in T1DM, n = 60 (69.8%) in HIV. The most frequent non-albicans Candida spp. were Candida kefyr (n = 6; 20%) in T1DM and Candida dubliniensis (n = 8; 9.3%) in HIV. Resistance to fluconazole was detected only in the HIV non-albicans Candida group (n = 8; 9.3%). All isolates were susc(..)

DOI: 10.5604/17331331.1197327

Published Online: 15-March-2016

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