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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology


ISSN: 1733-1331
eISSN: 2544-4646



Differentially Marked IncP-1β R751 Plasmids for Cloning via Recombineering and Conjugation
Colistin Resistance in Enterobacterales Strains – A Current View
Antibiotic Resistance Among Uropathogenic Escherichia coli

VOLUME 65 , ISSUE 2 (June 2016) - List of articles

Microbial Glycosylation of Flavonoids

Sandra Sordon/ Jarosław Popłoński/ Ewa Huszcza

Flavonoids constitute a large group of polyphenolic compounds naturally found in plants, which have a wide range of biological activity. Although flavonoids are beneficial to human health, their application is limited by their low bioavailability and poor water-solubility. Therefore, recently there has been a particular interest in glycosylated forms of flavonoids, which usually are better soluble, more stable, and more functional compared to their aglycones. Microbial transformation of natural (..)

DOI: 10.5604/17331331.1204473

Published Online: 07-June-2016

Relationship between ureB Sequence Diversity, Urease Activity and Genotypic Variations of Different Helicobacter pylori Strains in Patients with Gastric Disorders

Hossein Ghalehnoei/ Alireza Ahmadzadeh/ Nastaran Farzi/ Masoud Alebouyeh/ Hamid Asadzadeh Aghdaei/ Pedram Azimzadeh/ Mahsa Molaei/ Mohammad Reza Zali

Association of the severity of Helicobacter pylori induced diseases with virulence entity of the colonized strains was proven in some studies. Urease has been demonstrated as a potent virulence factor for H. pylori. The main aim of this study was investigation of the relationships of ureB sequence diversity, urease activity and virulence genotypes of different H. pylori strains with histopathological changes of gastric tissue in infected patients suffering from different gastric disorders. Analy(..)

DOI: 10.5604/17331331.1204761

Published Online: 07-June-2016

Interaction of Gram-Positive and Gram-Negative Bacteria with Ceramic Nanomaterials Obtained by Combustion Synthesis – Adsorption and Cytotoxicity Studies

Andrzej Borkowski/ Filip Owczarek/ Mateusz Szala/ Marek Selwet

This paper presents the interactions of Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas putida) bacteria with ceramic materials obtained by combustion synthesis. These studies were conducted based on an analysis of the adsorption of bacteria onto aggregates of ceramic materials in an aqueous suspension. The materials used in the studies were of a nanostructured nature and consisted mainly of carbides: silicon carbide (SiC) in the form of nanofibers (NFs) and nanorods (NRs), (..)

DOI: 10.5604/17331331.1204475

Published Online: 07-June-2016

Biocontrol of Gray Mold Decay in Pear by Bacillus amyloliquefaciens Strain BA3 and its Effect on Postharvest Quality Parameters

Hui Qu/ Longyu Zhao/ Fengchun Zhao/ Yufang Liu/ Zhengyou Yang

The economic losses caused by postharvest fruits diseases have attracted global attention. Traditional chemical fungicide could not meet the need of humans. In recent years, microbial agent which has begun to take the place of chemical fungicide comes into people’s vision. The aim of this paper was to investigate the potential of Bacillus amyloliquefaciens strain BA3 for its biocontrol capability on gray mold decay of pears and its effect on postharvest quality of pears. Compared with other trea(..)

DOI: 10.5604/17331331.1204476

Published Online: 07-June-2016

Enzymes Involved in Naproxen Degradation by Planococcus sp. S5

Danuta Wojcieszyńska/ Dorota Domaradzka/ Katarzyna Hupert-Kocurek/ Urszula Guzik

Naproxen is a one of the most popular non-steroidal anti-inflammatory drugs (NSAIDs) entering the environment as a result of high consumption. For this reason, there is an emerging need to recognize mechanisms of its degradation and enzymes engaged in this process. Planococcus sp. S5 is a gram positive strain able to degrade naproxen in monosubstrate culture (27%). However, naproxen is not a suf­ficient growth substrate for this strain. In the presence of benzoate, 4-hydroxybenzoic acid, 3,4-dih(..)

DOI: 10.5604/17331331.1204477

Published Online: 07-June-2016

Characterization of Bacteria Isolation of Bacteria from Pinyon Rhizosphere, Producing Biosurfactants from Agro-Industrial Waste

Arnoldo Wong-Villarreal/ Lizbeth Reyes-López/ Hipólito Corzo González/ Cristina Blanco González/ Gustavo Yáñez-Ocampo

Two hundred and fifty bacterial strains were isolated from pinyon rhizosphere and screened for biosurfactants production. Among them, six bacterial strains were selected for their potential to produce biosurfactants using two low cost wastes, crude glycerol and lactoserum, as raw material. Both wastes were useful for producing biosurfactants because of their high content in fat and carbohydrates. The six strains were identified by 16S rDNA with an identity percentage higher than 95%, three strai(..)

DOI: 10.5604/17331331.1204478

Published Online: 07-June-2016

Levels of Organic Compounds, Number of Microorganisms and Cadmium Accumulation in Festuca ovina Hydroponic Culture

Małgorzata Majewska/ Anna Słomka

Understanding the microbiological, biochemical and physiological aspects of phytoremediation of soil and water environments polluted to different degrees with heavy metals has very important theoretical and practical implications. In this study, a comparison was made between total cadmium concentration in root and shoot tissues as well as concentrations of particular fractions of Cd immobilized by roots of Festuca ovina (Sheep’s fescue) hydroponically cultivated in nutrient solutions supplemente(..)

DOI: 10.5604/17331331.1204479

Published Online: 07-June-2016

Prevalence of Parasitic Contamination in Salad Vegetables Collected from Supermarkets and Street Vendors in Amman and Baqa’a – Jordan

Yazan Ismail

One of the main ways in transmitting parasites to humans is through consuming contaminated raw vegetables. The aim of this study was to evaluate the prevalence of parasitological contamination (helminthes eggs, Giardia and Entamoeba histolytica cysts) of salad vegetables sold at supermarkets and street vendors in Amman and Baqa’a – Jordan. A total of 133 samples of salad vegetables were collected and examined for the prevalence of parasites. It was found that 29% of the samples were contaminated(..)

DOI: 10.5604/17331331.1204480

Published Online: 07-June-2016

Identification and Localization of β-D-Glucosidase from Two Typical Oenococcus oeni Strains

Yahui Li/ Yanhong Ma/ Kaihong Huang/ Hongzhi Zhang

β-D-glucosidase (βG) gene from Oenococcus oeni SD-2a and 31MBR was cloned, sequenced and analyzed, also intracellular βG of the two strains was further localized. The results showed that βG gene of the two strains was in high homology (> 99%) to reported βG gene, con­firming both strains possess βG activity at the molecular level. Intracellular βG of SD-2a is a mainly soluble protein, existing mostly in the cytoplasm and to some extent in the periplasm. While for 31MBR, intracellular βG is mainl(..)

DOI: 10.5604/17331331.1204481

Published Online: 07-June-2016

Characterization of Staphylococcal Cassette Chromosome mec (SCCmec) in Methicillin-Resistant Staphylococcus epidermidis Strains Isolated from Biomaterial-Associated Infections and their Antibiotic Resistance Patterns

Ewa Szczuka/ Karolina Bosacka/ Adam Kaznowski

This work aims to provide an insight into staphylococcal cassette chromosome mec elements and antibiotic resistance in clinical isolates of Staphylococcus epidermidis. The dominating type was SCCmec – IV. Fifteen isolates were assigned to SCCmec type III, two isolates to SCCmec type II. Most isolates were resistant to at least three of the non-β-lactam antibiotics tested. None of the strains exhibited resistance to new generation antibiotics, such as daptomycin and linezolid. Also, none of these(..)

DOI: 10.5604/17331331.1204482

Published Online: 07-June-2016

Qualitative and Quantitative Characteristics of Selected Bacterial Groups in Children with Inflammatory Bowel Diseases

Dorota Roszak/ Mirosława Gałęcka/ Wojciech Cichy/ Patrycja Szachta

The aim of the study was evaluation of qualitative and quantitative changes in bacterial ecosystem in 109 children with inflammatory bowel diseases. Stools obtained from patients were analysed for selected bacteria and concentration of faecal inflammatory markers (calprotectin, lactoferrin, M2-PK). The number of selected microorganisms depends on the level of clinical activity of disease and is correlated with faecal concentration of inflammatory markers. Differences in microflora disturbance, o(..)

DOI: 10.5604/17331331.1204483

Published Online: 07-June-2016

Cytotoxic and Bacteriostatic Activity of Nanostructured TiO2 Coatings

Alessandro Di Cerbo/ Federica Pezzuto/ Antonio Scarano

Nanostructures are structures, mainly synthetic (nanosurfaces, cylindrical nanotubes, and nanospheres), which range between 1–100 nm in at least one dimension and can be engineered to a wide range of physical properties. This paper aims to explore the bacteriostatic and cytotoxic characteristics of nano-TiO2 coated specimens of glass, stainless steel and ceramic with different thickness and roughness. The results show that stainless steel and glass specimens with a nano-TiO2 coating thickness of(..)

DOI: 10.5604/17331331.1204484

Published Online: 07-June-2016

Enteroviruses Associated with Aseptic Meningitis in Poland, 2011–2014

Magdalena Wieczorek/ Agnieszka Figas/ Arleta Krzysztoszek

A 4-year study (2011–2014) of patients with meningitis was performed. Out of the 686 cerebrospinal fluid samples, 465 (67.8%) were posi­tive for eneteroviruses using RT-PCR and out of 334 clinical samples, 216 (64.7%) were positive for enteroviruses using cell culture methods. The highest detection rate was observed in the summer and autumn. In total, 185 enteroviruses were identified by using neutralization test. Echovirus 6 and 30 were the most common (41.7% and 37.5% respectively). The highes(..)

DOI: 10.5604/17331331.1204485

Published Online: 07-June-2016

Monitoring of Virulence Genes, Drug-Resistance in Campylobacter coli Isolated from Golden Retrievers

Marek Selwet/ Mariola Galbas/ Ryszard Słomski/ Tomasz Cłapa/ Filip Porzucek

The investigation was performed on 75 of Golden Retriever puppies. Faecal samples were collected on the 42 day of the puppies life (con­trol). Probiotic preparation was administered on 43 day of the puppies life and 10 days after the application of the probiotic, faecal samples were collected again (on 53 day of puppies life). All isolates of Campylobacter coli isolated prior to the administration of the probiotic were found to contain the cadF gene responsible for adhesion, as well as, the flaA(..)

DOI: 10.5604/17331331.1204486

Published Online: 07-June-2016

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