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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology


ISSN: 1733-1331
eISSN: 2544-4646



Differentially Marked IncP-1β R751 Plasmids for Cloning via Recombineering and Conjugation
Colistin Resistance in Enterobacterales Strains – A Current View
Antibiotic Resistance Among Uropathogenic Escherichia coli

VOLUME 65 , ISSUE 3 (August 2016) - List of articles

Microbial Products and Biofertilizers in Improving Growth and Productivity of Apple – a Review

Walid Fediala Abd El-Gleel Mosa/ Lidia Sas-Paszt/ Mateusz Frąc/ Paweł Trzciński

The excessive use of mineral fertilizers causes many negative consequences for the environment as well as potentially dangerous effects of chemical residues in plant tissues on the health of human and animal consumers. Bio-fertilizers are formulations of beneficial micro­organisms, which upon application can increase the availability of nutrients by their biological activity and help to improve soil health. Microbes involved in the formulation of bio-fertilizers not only mobilize N and P but med(..)

DOI: 10.5604/17331331.1215599

Published Online: 26-August-2016

The Role of Staphylococcus aureus in Secondary Infections in Patients with Atopic Dermatitis (AD)

Aneta Buda/ Jacek Międzobrodzki

Staphylococcus aureus colonizes the mucous membrane of the nasal vestibule of a significant number of healthy people. These microorgan­isms are opportunistic pathogens, that in favorable conditions, may cause infections of various course, location or manifestation. Second­ary infections emerge in cases when other risk factors contribute to such a change. One of the diseases during which S. aureus changes its saprophytic character to a pathogenic one is atopic dermatitis (AD), an allergic skin co(..)

DOI: 10.5604/17331331.1215600

Published Online: 26-August-2016

Molecular Characterization of Shiga Toxin-Producing Escherichia coli Strains Isolated in Poland

Aleksandra Januszkiewicz/ Waldemar Rastawicki

Shiga toxin-producing Escherichia coli (STEC) strains also called verotoxin-producing E. coli (VTEC) represent one of the most important groups of food-borne pathogens that can cause several human diseases such as hemorrhagic colitis (HC) and hemolytic – uremic syndrome (HUS) worldwide. The ability of STEC strains to cause disease is associated with the presence of wide range of identified and putativevirulence factors including those encoding Shiga toxin. In this study, we examined the distribu(..)

DOI: 10.5604/17331331.1215601

Published Online: 26-August-2016

Genetic Variability and Proteome Profiling of a Radiation Induced Cellulase Mutant Mushroom Pleurotus florida

Chandran Sathesh-Prabu/ Young-Keun Lee

We report the genetic similarity changes between a mutant mushroom (Pleurotus florida, designated as PfCM4) having increased cellulo­lytic activity developed through radiation mutagenesis and its wild type by amplified fragment length polymorphism (AFLP). On average, 23 AFLP fragments were amplified per primer combination, and a total of 286 polymorphic fragments (78.57% polymorphism) with maxi­mal fragment length of 1365 base pairs (bp) were obtained. The genetic similarity between wild type an(..)

DOI: 10.5604/17331331.1215606

Published Online: 26-August-2016

Partial Characterization of Bacteriocin Produced by Halotolerant Pediococcus acidilactici Strain QC38 Isolated from Traditional Cotija Cheese

Aurea I. Morales-Estrada/ Ahidé López-Merino/ Nestor Gutierrez-Mendez/ Enrico A. Ruiz/ Araceli Contreras-Rodríguez

During a screening of lactic acid bacteria producing bacteriocin from Cotija cheese, the strain QC38 was isolated. Based on the 16S rRNA gene nucleotide sequencing (516 pb accession no KJ210322) and phylogenetic analysis, the isolate was identified as Pediococcus acidilactici. Neutralized cell-free supernatant was tested for antimicrobial activity against 17 Gram-negative and Gram-positive pathogens. Growth inhibition was achieved against Listeria monocytogenes (supplier or indication or source)(..)

DOI: 10.5604/17331331.1215607

Published Online: 26-August-2016

Simultaneous Biodegradation of Phenol and n-Hexadecane by Cryogel Immobilized Biosurfactant Producing Strain Rhodococcus wratislawiensis BN38

Alexander E. Hristov/ Nelly E. Christova/ Lyudmila V. Kabaivanova/ Lilyana V. Nacheva/ Ivanka B. Stoineva/ Petar D. Petrov

The capability of the biosurfactant-producing strain Rhodococcus wratislawiensis BN38 to mineralize both aromatic and aliphatic xeno­biotics was proved. During semicontinuous cultivation 11 g/l phenol was completely degraded within 22 cycles by Rhodococcus free cells. Immobilization in a cryogel matrix was performed for the first time to enhance the biodegradation at multiple use. A stable simultaneous hydrocarbon biodegradation was achieved until the total depletion of 20 g/l phenol and 20 g/l (..)

DOI: 10.5604/17331331.1215608

Published Online: 26-August-2016

Complex Biochemical Analysis of Fruiting Bodies from Newly Isolated Polish Flammulina velutipes Strains

Monika Osińska-Jaroszuk/ Magdalena Jaszek/ Justyna Sulej/ Dawid Stefaniuk/ Monika Urbaniak/ Marek Siwulski/ Grzegorz Janusz

The present study examined Polish strains of Flamulina velutipes as a potential source of nutraceuticals and found that their nutritional value is dependent on the fruiting bodies gathering time. To prove the above hypothesis protein, carbohydrate and phenolic substances concen­tration were determined. Moreover, catalase, superoxide dismutase, cellobiose dehydrogenase activities were assayed. In order to prove the healing properties of Enoki fruiting bodies the obtained extracts were tested for (..)

DOI: 10.5604/17331331.1215609

Published Online: 26-August-2016

Prevalence of Biofilm Formation and Wide Distribution of Virulence Associated Genes among Vibrio spp. Strains Isolated from the Monastir Lagoon, Tunisia

Badreddine Mechri/ Amel Medhioub/ Mohamed Nejib Medhioub/ Mahjoub Aouni

In the current study, 65 Vibrio spp. were isolated from the Monastir lagoon water, were characterized phenotypically and genotypically. In addition, we looked for the presence of three Vibrio parahaemolyticus virulence genes (tlh, trh and tdh) and ten Vibrio cholerae virulence genes (ctxA, vpi, zot, ace, toxR, toxT, tosS, toxRS, tcpA and cpP). We also investigated the antibiotic susceptibilities and the adherence ability of the identified strains to abiotic material and to biotic surfaces. The c(..)

DOI: 10.5604/17331331.1215610

Published Online: 26-August-2016

Distribution and Identification of Endophytic Streptomyces Species from Schima wallichii as Potential Biocontrol Agents against Fungal Plant Pathogens

Ajit Kumar Passari/ Vineet Kumar Mishra/ Vijai Kumar Gupta/ Ratul Saikia/ Bhim Pratap Singh

The prospective of endophytic microorganisms allied with medicinal plants is disproportionally large compared to those in other biomes. The use of antagonistic microorganisms to control devastating fungal pathogens is an attractive and eco-friendly substitute for chemi­cal pesticides. Many species of actinomycetes, especially the genus Streptomyces, are well known as biocontrol agents. We investigated the culturable community composition and biological control ability of endophytic Streptomyces (..)

DOI: 10.5604/17331331.1215611

Published Online: 26-August-2016

Characterization of Rhizobial Bacteria Nodulating Astragalus corrugatus and Hippocrepis areolata in Tunisian Arid Soils

Mosbah Mahdhi/ Nadia Houidheg/ Neji Mahmoudi/ Abdelhakim Msaadek/ Mokhtar Rejili/ Mohamed Mars

Fifty seven bacterial isolates from root nodules of two spontaneous legumes (Astragalus corrugatus and Hippocrepis areolata) growing in the arid areas of Tunisia were characterized by phenotypic features, 16S rDNA PCR-RFLP and 16S rRNA gene sequencing. Phenotypically, our results indicate that A. corrugatus and H. areolata isolates showed heterogenic responses to the different phenotypic features. All isolates were acid producers, fast growers and all of them used different compounds as sole car(..)

DOI: 10.5604/17331331.1215612

Published Online: 26-August-2016

Characterization of a Highly Enriched Microbial Consortium Reductively Dechlorinating 2,3-Dichlorophenol and 2,4,6-Trichlorophenol and the Corresponding cprA Genes from River Sediment

Wael S. El-Sayed

Anaerobic reductive dechlorination of 2,3-dichlorophenol (2,3DCP) and 2,4,6-trichlorophenol (2,4,6TCP) was investigated in microcosms from River Nile sediment. A stable sediment-free anaerobic microbial consortium reductively dechlorinating 2,3DCP and 2,4,6TCP was established. Defined sediment-free cultures showing stable dechlorination were restricted to ortho chlorine when enriched with hydrogen as the electron donor, acetate as the carbon source, and either 2,3-DCP or 2,4,6-TCP as electron ac(..)

DOI: 10.5604/17331331.1215613

Published Online: 26-August-2016

The Effect of Local Platelet Rich Plasma Therapy on the Composition of Bacterial Flora in Chronic Venous Leg Ulcer

Witold Woźniak/ Monika Tarnas/ Tomasz Miłek/ R. Krzysztof Mlosek/ Piotr Ciostek

Microbial colonisation of chronic venous ulcers and synergism between bacterial species slow down the healing process. The study aimed at performing qualitative analysis of microbial flora in venous leg ulcers treated with platelet rich plasma (PRP). Twenty two women and twelve men aged 47–90 years were treated with PRP at our department between 2012 and 2015. Ulcer cultures collected before and after PRP therapy yielded 83 and 110 microbial isolates, respectively, of Gram positive, Gram negativ(..)

DOI: 10.5604/17331331.1215614

Published Online: 26-August-2016

Search of the Antimicrobial Potential of Benzimidazole Derivatives

Monika Janeczko/ Zygmunt Kazimierczuk/ Andrzej Orzeszko/ Andrzej Niewiadomy/ Ewa Król/ Ryszard Szyszka/ Maciej Masłyk

A broad series of 4,5,6,7-tetrahalogenated benzimidazoles and 4-(1H-benzimidazol-2-yl)-benzene-1,3-diol derivatives was tested against selected bacteria and fungi. For this study three plant pathogens Colletotrichum sp., Fusarium sp., and Sclerotinia sp., as well as Staphylo­coccus sp., Enterococcus sp., Escherichia sp., Enterobacter sp., Klebsiella spp. , and Candida spp. as human pathogens were used. MIC values and/or area of growth reduction method were applied in order to compare the activit(..)

DOI: 10.5604/17331331.1215615

Published Online: 26-August-2016

Pexophagy in Penicillin G Secretion by Penicillium chrysogenum PQ-96

Wiesław Kurzątkowski/ Anita Gębska-Kuczerowska

Penicillin G oversecretion by Penicillium chrysogenum PQ-96 is associated with a strictly adjusted cellular organization of the mature and senescent mycelial cells. Abundant vacuolar phagy and extended cellular vacuolization combined with vacuolar budding resulting in the formation of vacuolar vesicles that fuse with the cell membrane are the most important characteristic features of those cells. We suggest as follows: if the peroxisomes are integrated into vacuoles, the penicillin G formed in p(..)

DOI: 10.5604/17331331.1215616

Published Online: 26-August-2016

Endophytic Detection in Selected European Herbal Plants

Agata Goryluk-Salmonowicz/ Monika Piórek/ Hanna Rekosz-Burlaga/ Marcin Studnicki/ Mieczysław Błaszczyk

A total of 181 cultivable endophytic bacterial isolates were collected from stems of 13 species of herbs inhabiting Europe (Poland): Chelido­nium majus L., Elymus repens L., Erigeron annuus L., Euphrasia rostkoviana Hayne, Foeniculum vulgare L., Geranium pratense L., Humulus lupulus L., Matricaria chamomilla L., Mentha arvensis L., Papaver rhoeas L., Rosmarinus officinalis L., Solidago gigantea L. and Vinca minor L. The isolates were screened for their antifungal activity and fifty three were fo(..)

DOI: 10.5604/17331331.1215617

Published Online: 26-August-2016

Effects of Selected Herbicides on Growth and Nitrogen Fixing Activity of Stenotrophomonas maltophilia (Sb16)

Armita Nahi/ Radziah Othman/ Dzolkhifli Omar/ Mahdi Ebrahimi

A study was carried out to determine the effects of paraquat, pretilachlor and 2, 4-D on growth and nitrogen fixing activity of Stenotropho­monas maltophilia (Sb16) and pH of Jensen’s N-free medium. The growth of Sb16 and pH of medium were significantly reduced with full (X) and double (2X) doses of tested herbicides, but nitrogen fixing activity was decreased by 2X doses. The nitrogenase activity had the highest value in samples treated with 1/2X of 2, 4-D on fifth incubation day, but 2X of 2, (..)

DOI: 10.5604/17331331.1215618

Published Online: 26-August-2016

Basidiospore and Protoplast Regeneration from Raised Fruiting Bodies of Pathogenic Ganoderma boninense

Nisha Thopla Govender/ Maziah Mahmood/ Idris Abu Seman/ Wong Mui-Yun

Ganoderma boninense, a phytopathogenic white rot fungus had sought minimal genetic characterizations despite huge biotechnological poten­tials. Thus, efficient collection of fruiting body, basidiospore and protoplast of G. boninense is described. Matured basidiocarp raised under the glasshouse conditions yielded a total of 8.3 × 104 basidiospores/ml using the low speed centrifugation technique. Mycelium aged 3-day-old treated under an incubation period of 3 h in lysing enzyme from Trichoderma ha(..)

DOI: 10.5604/17331331.1215619

Published Online: 26-August-2016

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