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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology


ISSN: 1733-1331
eISSN: 2544-4646



Differentially Marked IncP-1β R751 Plasmids for Cloning via Recombineering and Conjugation
Colistin Resistance in Enterobacterales Strains – A Current View
Antibiotic Resistance Among Uropathogenic Escherichia coli

VOLUME 66 , ISSUE 1 (March 2017) - List of articles

From Latent Tuberculosis Infection to Tuberculosis. News in Diagnosticsb (QuantiFERON-Plus)

Dagmara I. Borkowska/ Agnieszka M. Napiórkowska/ Sylwia A. Brzezińska/ Monika Kozińska/ Anna T. Zabost/ Ewa M. Augustynowicz-Kopeć

It is estimated that one third of the world’s population have latent tuberculosis infection and that this is a significant reservoir for future tuberculosis cases. Most cases occur within two years following initial infection. The identification of individuals with latent tuberculosis infection is difficult due to the lack of an ideal diagnostic assay and incomplete understanding of latent infection. Currently, there are three tests: the oldest tuberculin skin test, T-SPOT.TB and the latest Quan(..)

DOI: 10.5604/17331331.1234987

Published Online: 30-March-2017

Application of Metagenomic Analyses in Dentistry as a Novel Strategy Enabling Complex Insight into Microbial Diversity of the Oral Cavity

Aleksandra Burczyńska/ Łukasz Dziewit/ Przemysław Decewicz/ Izabela Strużycka/ Marta Wróblewska

The composition of the oral microbiome in healthy individuals is complex and dynamic, and depends on many factors, such as anatomi­cal location in the oral cavity, diet, oral hygiene habits or host immune responses. It is estimated at present that worldwide about 2 billion people suffer from diseases of the oral cavity, mainly periodontal disease and dental caries. Importantly, the oral microflora involved in local infections may spread and cause systemic, even life-threatening infections. In se(..)

DOI: 10.5604/17331331.1234988

Published Online: 30-March-2017

Deletion of atoR from Streptococcus pyogenes Results in Hypervirulence in a Mouse Model of Sepsis and is LuxS Independent

Izabela Sitkiewicz/ James M. Musser

Group A Streptococcus (GAS) is a Gram-positive human pathogen that causes a variety of diseases ranging from pharyngitis to life-threaten­ing streptococcal toxic shock syndrome. Recently, several global gene expression analyses have yielded extensive new information regarding the regulation of genes encoding known and putative virulence factors in GAS. A microarray analysis found that transcription of the GAS gene M5005_Spy_1343 was significantly increased in response to interaction with human p(..)

DOI: 10.5604/17331331.1234989

Published Online: 30-March-2017

Expression of the Fluoroquinolones Efflux Pump Genes acrA and mdfA in Urinary Escherichia coli Isolates

Sarah M. Abdelhamid/ Rania R. Abozahra

Escherichia coli is one of the most frequent causes of urinary tract infections. Efflux system overexpression is reported to contribute to E. coli resistance to several antibiotics. Our aim in this study was to investigate the relation between antibiotic resistance and the expres­sion of the efflux pump genes acrA and mdfA in E. coli by real-time reverse transcription-PCR. We tested the in vitro susceptibilities to 12 antibiotics in 28 clinical isolates of E. coli obtained from urine samples. We(..)

DOI: 10.5604/17331331.1234990

Published Online: 30-March-2017

Antimicrobial Effects of Platelet-rich Plasma against Selected Oral and Periodontal Pathogens

Reham L. Aggour/ Lina Gamil

Antimicrobial properties of platelet rich plasma (PRP) against various microorganisms have been recently pointed out. PRP could be an alternative to conventional antibiotics in preventing oral and periodontal infections. We examined whether PRP has in vitro antimicrobial properties against Aggregatibacter actinomycetemcomitans, Prophyromonas gingivalis, Staphylococcus aureus and Candida albicans. PRP and platelet-poor plasma (PPP) were obtained from whole blood of 10 healthy volunteers and 10 pe(..)

DOI: 10.5604/17331331.1235227

Published Online: 30-March-2017

A Comparative Study: Taxonomic Grouping of Alkaline Protease Producing Bacilli

Nilgun Tekin/ Arzu Coleri Cihan/ Basar Karaca/ Cumhur Cokmus

Alkaline proteases have biotechnological importance due to their activity and stability at alkaline pH. 56 bacteria, capable of growing under alkaline conditions were isolated and their alkaline protease activities were carried out at different parameters to determine their optimum alkaline protease production conditions. Seven isolates were showed higher alkaline protease production capacity than the reference strains. The highest alkaline protease producing isolates (103125 U/g), E114 and C265(..)

DOI: 10.5604/17331331.1234992

Published Online: 30-March-2017

Isolation and Characterization of Phosphate-Solubilizing Bacteria from Mushroom Residues and their Effect on Tomato Plant Growth Promotion

Jian Zhang/ Pengcheng Wang/ Ling Fang/ Qi-An Zhang/ Congsheng Yan/ Jingyi Chen

Phosphorus is a major essential macronutrient for plant growth, and most of the phosphorus in soil remains in insoluble form. Highly efficient phosphate-solubilizing bacteria can be used to increase phosphorus in the plant rhizosphere. In this study, 13 isolates were obtained from waste mushroom residues, which were composed of cotton seed hulls, corn cob, biogas residues, and wood flour. NBRIP solid medium was used for isolation according to the dissolved phosphorus halo. Eight isolates produce(..)

DOI: 10.5604/17331331.1234993

Published Online: 30-March-2017

Dipicolinic Acid Release and the Germination of Alicyclobacillus acidoterrestris Spores under Nutrient Germinants

Izabela Porębska/ Barbara Sokołowska/ Łukasz Woźniak

The presence of Alicyclobacillus, a thermoacidophilic and spore-forming bacterium, in acidic fruit juices poses a serious problem for the processing industry. A typical sign of spoilage in contaminated juices is a characteristic phenolic off-flavour associated with the produc­tion of guaiacol. Spores are formed in response to starvation and in a natural environment re-access the nutrients, e.g.: L-alanine and AGFK – a mixture of asparagine, glucose, fructose and potassium, triggers germination. (..)

DOI: 10.5604/17331331.1234994

Published Online: 30-March-2017

Microbial Biomass and Enzymatic Activity of the Surface Microlayer and Subsurface Water in Two Dystrophic Lakes

Iwona Kostrzewska-Szlakowska/ Bartosz Kiersztyn

Nutrient and organic matter concentration, microbial biomass and activities were studied at the surface microlayers (SML) and subsurface waters (SSW) in two small forest lakes of different water colour. The SML in polyhumic lake is more enriched with dissolved inorganic nitro­gen (0.141 mg l–1) than that of oligohumic lake (0.124 mg l–1), the former also contains higher levels of total nitrogen (2.66 mg l–1). Higher activities of lipase (Vmax 2290 nmol l–1 h–1 in oligo- and 6098 in polyhumic) an(..)

DOI: 10.5604/17331331.1234995

Published Online: 30-March-2017

Suppressive Effect of Trichoderma spp. on toxigenic Fusarium species

Lidia Błaszczyk/ Aneta Basińska-Barczak/ Hanna Ćwiek-Kupczyńska/ Karolina Gromadzka/ Delfina Popiel/ Łukasz Stępień

The aim of the present study was to examine the abilities of twenty-four isolates belonging to ten different Trichoderma species (i.e., Trichoderma atroviride, Trichoderma citrinoviride, Trichoderma cremeum, Trichoderma hamatum, Trichoderma harzianum, Trichoderma koningiopsis, Trichoderma longibrachiatum, Trichoderma longipile, Trichoderma viride and Trichoderma viridescens) to inhibit the mycelial growth and mycotoxin production by five Fusarium strains (i.e., Fusarium avenaceum, Fusarium cerea(..)

DOI: 10.5604/17331331.1234996

Published Online: 30-March-2017

Screening and Identification of Yeasts Antagonistic to Pathogenic Fungi Show a Narrow Optimal pH Range for Antagonistic Activity

Pei-Hua Chen/ Jui-Yu Chou

Microbes have evolved ways of interference competition to gain advantage over their ecological competitors. The use of secreted antagonistic compounds by yeast cells is one of the prominent examples. Although this killer behavior has been thoroughly studied in laboratory yeast strains, our knowledge of the antagonistic specificity of killer effects in nature remains limited. In this study, yeast strains were collected from various niches and screened for antagonistic activity against one toxin-s(..)

DOI: 10.5604/17331331.1234997

Published Online: 30-March-2017

Use of Amplification Fragment Length Polymorphism to Genotype Pseudomonas stutzeri Strains Following Exposure to Ultraviolet Light A

Lisa Lombardi/ Marina Zoppo/ Cosmeri Rizzato/ Colin Gerard Egan/ Roberto Scarpato/ Arianna Tavanti

Changes in ultraviolet light radiation can act as a selective force on the genetic and physiological traits of a microbial community. Two strains of the common soil bacterium Pseudomonas stutzeri, isolated from aquifer cores and from human spinal fluid were exposed to ultraviolet light. Amplification length polymorphism analysis (AFLP) was used to genotype this bacterial species and evaluate the effect of UVA-exposure on genomic DNA extracted from 18 survival colonies of the two strains compared(..)

DOI: 10.5604/17331331.1234998

Published Online: 30-March-2017

Vital Staining of Bacteria by Sunset Yellow Pigment

Peng Gao/ Chengming Sun/ Yuxia Li/ Xiaohua Zou/ Xun Wu/ Yan Ling/ Caifu Luan/ Huipeng Chen

In this study, we describe a method for discriminating pathogenic bacteria with a dye. First, we determined that among several colours tested, the sunset yellow pigment easily coloured Escherichia coli bacteria yellow. Next, we demonstrated that E. coli O157:H7, Shigella flexneri O301, Staphylococcus aureus and Bacillus subtilis could all be well marked by sunset yellow pigment. Finally, we performed bacterial viability assays and found there was no effect on bacterial growth when in co-culture (..)

DOI: 10.5604/17331331.1234999

Published Online: 30-March-2017

Outpatient Antibiotic Consumption Fluctuations in a View of Unreasonable Antibacterial Therapy

Marcin Ciszewski/ Tomasz Czekaj/ Eligia M. Szewczyk

Unreasonable antibacterial therapy is suspected to be the main reason of emergence of multi-resistant bacteria. The connection between seasonal variability of antibiotic use and reasonable antibacterial therapy has been described. We examined the issue basing on the data obtained from the primary care system in Szczecin (Poland) in order to verify the situation in this region of Central Europe. Increase in antibiotic consumption in a viral infection season was proved to be statistically signific(..)

DOI: 10.5604/17331331.1235000

Published Online: 30-March-2017

Susceptibility of Vascular Implants to Colonization in vitro by Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis and Pseudomonas aeruginosa

Witold Woźniak/ Aleksandra Kozińska/ Piotr Ciostek/ Izabela Sitkiewicz

We compared association of Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Enterococcus faecalis with nine vascular implants after co-culture. Vascular implants were composed of various materials such as warp knitted polyester (with or without gelatin and silver ions), expanded polytetrafluoroethylene and biological materials – surface treated porcine pericardial patch and Omniflow II. The lowest overall number of associated bacteria was detected for polytetrafluoro(..)

DOI: 10.5604/17331331.1235001

Published Online: 30-March-2017

Antiviral Resistance of Splenocytes in Aged Mice

Ewa Zaczyńska/ Jolanta Artym/ Maja Kocięba/ Timo Burster/ Marian Kruzel/ Maria Paprocka/ Michał Zimecki

We compared the susceptibility to viral infection of splenocytes, isolated from young versus old CBA mice, and evaluated the antiviral actions of lactoferrin in splenocytes infected with Encephalomyocarditis virus (EMCV). Recombinant mouse lactoferrin (rmLF) and bovine lactoferrin (bLF) were used. There were no differences in the susceptibility to EMCV infection in the studied age categories. Both types of lactoferrins were protective in young and old mice. The study confirmed the undisturbed vi(..)

DOI: 10.5604/17331331.1235002

Published Online: 30-March-2017

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