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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology


ISSN: 1733-1331
eISSN: 2544-4646



Differentially Marked IncP-1β R751 Plasmids for Cloning via Recombineering and Conjugation
Colistin Resistance in Enterobacterales Strains – A Current View
Antibiotic Resistance Among Uropathogenic Escherichia coli

VOLUME 66 , ISSUE 2 (June 2017) - List of articles

Biochar-Rhizosphere Interactions – a Review

Sławomir Głuszek/ Lidia Sas-Paszt/ Beata Sumorok/ Ryszard Kozera

Biochar is a solid material of biological origin obtained from biomass carbonization, designed as a mean to reduce greenhouse gases emis­sion and carbon sequestration in soils for a long time. Biochar has a wide spectrum of practical utilization and is applied as a promising soil improver or fertilizer in agriculture, or as a medium for soil or water remediation. Preparations of biochar increase plant growth and yielding when applied into soil and also improve plant growth conditions, mainly bio(..)

DOI: 10.5604/01.3001.0010.6288

Published Online: 28-June-2017

Genetic Characterization of a Novel Composite Transposon Carrying armA and aac(6)-Ib Genes in an Escherichia coli Isolate from Egypt

Mona T. Kashef/ Omneya M. Helmy

Aminoglycosides are used in treating a wide range of infections caused by Gram-positive and Gram-negative bacteria; however, amino­glycoside resistance is common and occurs by several mechanisms. Among these mechanisms is bacterial rRNA methylation by the 16S rRNA methyl transferase (16S-RMTase) enzymes; but data about the spread of this mechanism in Egypt are scarce. Cephalosporins are the most commonly used antimicrobial agents in Egypt; therefore, this study was conducted to determine the fre(..)

DOI: 10.5604/01.3001.0010.7835

Published Online: 28-June-2017

Trends of Bloodstream Infections in a University Greek Hospital during a Three-Year Period: Incidence of Multidrug-Resistant Bacteria and Seasonality in Gram-negative Predominance

Fevronia Kolonitsiou/ Matthaios Papadimitriou-Olivgeris/ Anastasia Spiliopoulou/ Vasiliki Stamouli/ Vasileios Papakostas/ Eleni Apostolopoulou/ Christos Panagiotopoulos/ Markos Marangos/ Evangelos D. Anastassiou/ Myrto Christofidou/ Iris Spiliopoulou

The aim of the study was to assess the epidemiology, the incidence of multidrug-resistant bacteria and bloodstream infections’ (BSIs) seasonality in a university hospital. This retrospective study was carried out in the University General Hospital of Patras, Greece, during 2011–13 y. Blood cultures from patients with clinical presentation suggestive of bloodstream infection were performed by the BacT/ALERT System. Isolates were identified by Vitek 2 Advanced Expert System. Antibiotic susceptibil(..)

DOI: 10.5604/01.3001.0010.7834

Published Online: 28-June-2017

Effect of Lyophilization on Survivability and Growth Kinetic of Trichoderma Strains Preserved on Various Agriculture By-Products

Danuta Witkowska/ Katarzyna Buska-Pisarek/ Wojciech Łaba/ Michał Piegza/ Anna Kancelista

Growth kinetics of four Trichoderma strains was tested on lignocellulosic by-products in solid state fermentation (SSF). The strains were also analyzed for their survival rate and growth after lyophilization on these carriers. All applied monocomponent and bicomponent media were substrates for the production and preservation of Trichoderma biomass. However, the maximum number of colony forming units (CFU/g dm) was acquired on bicomponent media based on dried grass and beet pulp or grass with cor(..)

DOI: 10.5604/01.3001.0010.4361

Published Online: 28-June-2017

Gene Expression during BTEX Biodegradation by a Microbial Consortium Acclimatized to Unleaded Gasoline and a Pseudomonas putida Strain (HM346961) Isolated from It

Jesús A. Morlett Chávez/ Jorge Á. Ascacio Martínez/ William E. Haskins/ Hugo A. Barrera Saldaña/ Karim Acuña Askar

Pseudomonas putida strain (HM346961) was isolated from a consortium of bacteria acclimatized to unleaded gasoline-contaminated water. The consortium can efficiently remove benzene, toluene, ethylbenzene and xylene (BTEX) isomers, and a similar capability was observed with the P. putida strain. Proteome of this strain showed certain similarities with that of other strains exposed to the hydrocarbon com­pounds. Furthermore, the toluene di-oxygenase (tod) gene was up-regulated in P. putida strain w(..)

DOI: 10.5604/01.3001.0010.7836

Published Online: 28-June-2017

The Diversity, Growth Promoting Abilities and Anti-microbial Activities of Bacteria Isolated from the Fruiting Body of Agaricus bisporus

Quanju Xiang/ Lihua Luo/ Yuhuan Liang/ Qiang Chen/ Xiaoping Zhang/ Yunfu Gu

Agaricus bisporus plays an important role in ecological processes and is one of the most widely cultivated mushrooms worldwide. Mushroom growth-promoting bacteria have been isolated from casing soil and compost, but microorganisms in the fruiting body have received only a little attention. To get an overview of phylogenetic diversity of microorganisms in the fruiting body of A. bisporus, as well as to screen antimicrobial and mushroom growth-promoting strains, and eventually intensify mushroom p(..)

DOI: 10.5604/01.3001.0010.7837

Published Online: 28-June-2017

Molecular Study of Indigenous Bacterial Community Composition on Exposure to Soil Arsenic Concentration Gradient

Semanti Basu/ Tanima Paul/ Priya Yadav/ Abhijit Debnath/ Keka Sarkar

Community structure of bacteria present in arsenic contaminated agricultural soil was studied with qPCR (quantitative PCR) and DGGE (Denaturing Gradient Gel Electrophoresis) as an indicator of extreme stresses. Copy number of six common bacterial taxa (Acidobacteria, Actinobacteria, α-, β- and γ-Proteobacteria, Firmicutes) was calculated using group specific primers of 16S rDNA. It revealed that soilcontaminated with low concentration of arsenic was dominated by both Actinobacteria and Proteobac(..)

DOI: 10.5604/01.3001.0010.7838

Published Online: 28-June-2017

Safety Evaluation of Enterocin Producer Enterococcus sp. Strains Isolated from Traditional Turkish Cheeses

Mine Avci/ Banu Özden Tuncer

The purpose of this study was to determine the antimicrobial activity and occurrence of bacteriocin structural genes in Enterococcus spp. isolated from different cheeses and also investigate some of their virulence factors. Enterococcus strains were isolated from 33 different cheeses. Enterococcus faecium (6 strains) and Enterococcus faecalis (5 strains) enterocin-producing strains were identified by 16S rDNA analyses. Structural genes entA, entB, entP and entX were detected in some isolates. Mu(..)

DOI: 10.5604/01.3001.0010.7839

Published Online: 28-June-2017

Intracellular Siderophore Detection in an Egyptian, Cobalt-Treated F. solani Isolate Using SEM-EDX with Reference to its Tolerance

Farrag M. Rasha

An Egyptian, plant pathogenic Fusarium solani isolate was grown on cobalt concentrations of 0, 50, 200, 500, 800, and 1000 ppm. The iso­late survived concentrations up to 800 ppm, however failed to grow at 1000 ppm. Morphology and elemental analysis of the isolate under the investigated Co concentrations were studied using Scanning electron microscopy (SEM) and energy dispersive X-ray microanalysis (EDX). The isolate reserved its morphology up to a concentration of 200 ppm. Morphological distort(..)

DOI: 10.5604/01.3001.0010.7856

Published Online: 28-June-2017

In vitro Interaction between Fumonisin B1 and the Intestinal Microflora of Pigs

Huu Anh Dang/ Attila Zsolnai/ Melinda Kovacs/ István Bors/ András Bónai/ Brigitta Bóta/ Judit Szabó-Fodor

The caecal chyme of pigs was incubated anaerobically in McDougall buffer with and without fumonisin B1 (5 μg/ml) for 0, 24 and 48 h. The plate count agar technique was applied for enumerating the amount of bacteria including aerobic, anaerobic bacteria, coliform, Escherichia coli and Lactobacillus sp. The quantitative polymerase chain reaction was also performed to estimate the number of copies of the total bacteria, Lactobacillus, Bacteroides and Prevotella. No significant differences in the am(..)

DOI: 10.5604/01.3001.0010.7858

Published Online: 28-June-2017

Early KPC-Producing Klebsiella pneumoniae Bacteremia among Intensive Care Unit Patients Non-Colonized upon Admission

Matthaios Papadimitriou-Olivgeris/ Fotini Fligou/ Iris Spiliopoulou/ Christina Bartzavali/ Vasiliki Dodou/ Sophia Vamvakopoulou/ Kyriaki Koutsileou/ Anastasia Zotou/ Evangelos D. Anastassiou/ Myrto Christofidou/ Markos Marangos

Among 140 patients colonized by KPC-producing Klebsiella pneumoniae (KPC-Kp) between fourth and seventh day of Intensive Care Unit stay, 24 developed bacteraemia immediately after colonization. Colistin-resistance of the colonizing isolate was the factor significantly associated with early KPC-Kp bacteraemia (P < 0.001; OR 6.6, 95% CI 2.4–18.4), a worrisome finding since infections by colistin-resistant isolates is associated with increased mortality due to limited remaining therapeutic options.(..)

DOI: 10.5604/01.3001.0010.7863

Published Online: 28-June-2017

An Improve Protocol for PCR Using LM1 and LM2 Primers for Listeria monocytogenes Detection in Food Matrices

Angélica Godínez-Oviede/ Gerardo M. Nava/ Sofía M. Arvizu-Medrano/ Montserrat Hernández-Iturriaga

Several studies have observed that use of a conventional PCR protocol with primers LM1 and LM2 for the identification of the hlyA gene of Listeria monocytogenes generates non-specific PCR amplifications and false positives. For this reason, in this study we provide a modified PCR protocol that improves the specificity of the results obtained with LM1 and LM2 primers.

DOI: 10.5604/01.3001.0010.7869

Published Online: 28-June-2017

Bactericidal Activity of Octenidine to Various Genospecies of Borrelia burgdorferi, Sensu Lato Spirochetes in Vitro and in Vivo

Stanisława Tylewska-Wierzbanowska/ Urszula Roguska/ Grażyna Lewandowska/ Tomasz Chmielewski

The aim of our studies was to invent a reliable method for detection of the bactericidal activity of disinfectants against Borrelia burgdorferi in suspension (in vitro) and in cell line cultures (in vivo). In the suspension method, 0.01% octenidine at 20°C and 35°C was bactericidalto Borrelia afzeli; Borrelia garini, B. burgdorferi sensu stricto after 5 minutes treatment. Increase of the temperature to 35°C speed up the bactericidal effect to 1 minute. The bactericidal action of octenidine towar(..)

DOI: 10.5604/01.3001.0010.7874

Published Online: 28-June-2017

Erythromycin or Clindamycin – is it Still an Empirical Therapy against Streptococcus agalactiae in Patients Allergic to Penicillin?

Piotr Leszczyński/ Beata Sokół-Leszczyńska/ Bronisława Pietrzak/ Anna Sawicka-Grzelak/ Mirosław Wielgoś

Retrospective analysis of Streptococcus agalactiae antibiotic susceptibility isolated in 2010–2013 was performed. Penicillin was still the first-line antibiotic. Due to the high percentage of strains resistant to erythromycin and clindamycin empirical treatment with these antibiotics may not be effective. Lower resistance rate to erythromycin and clindamycin among strains isolated from infected pregnant women and newborns were observed than among strains isolated from samples from patients hospi(..)

DOI: 10.5604/01.3001.0010.7878

Published Online: 28-June-2017

Influence of Pseudomonas and Bacillus Strains Isolated from Lolium perenne Rhizospheric Soil in Vojvodina (Serbia) on Planth Growth and Soil Microbial Communities

Dragana R. Stamenov/ Simonida Djuric/ Timea Hajnal-Jafari/ Snežana Andjelkovic

The aim of this study was the isolation of Pseudomonas sp. and Bacillus sp. strains from rhizospheric soil and monitoring the impact of two isolates denoted as P12 (Pseudomonas sp.) and B1 (Bacillus sp.) on the parameters of English ryegrass (Lolium perenne) yield and activity of the soil microbial communities. During 2012–2014, a plot experiment was set up following the randomized block system. Better effect on the plant growth was recorded with the use of Pseudomonas sp. P12 isolate than with (..)

DOI: 10.5604/01.3001.0010.7879

Published Online: 28-June-2017

Betaine Improves Polymer-Grade D-Lactic Acid Production by Sporolactobacillus inulinus Using Ammonia as Green Neutralizer

Guoping Lv/ Chengchuan Che/ Li Li/ Shujing Xu/ Wanyi Guan/ Baohua Zhao/ Jiansong Ju

The traditional CaCO3-based fermentation process generates huge amount of insoluble waste. To solve this problem, we have developed an efficient and green D-lactic acid fermentation process by using ammonia as neutralizer. The 106.7 g/l of D-lactic acid production and 0.89 g/g of consumed sugar were obtained by Sporolactobacillus inulinus CASD with a high optical purity of 99.7% by adding 100 mg/l betaine in the simple batch fermentation. The addition of betaine was experimentally proven to prot(..)

DOI: 10.5604/01.3001.0010.7880

Published Online: 28-June-2017

The Serological Surveillance of Hepatitis E virus among Hunters and Foresters in Eastern Poland

Marcin Weiner/ Małgorzata Tokarska-Rodak/ Dorota Plewik/ Anna Pańczuk/ Adam Szepeluk/ Monika Krajewska

The aim of the study was the analysis of the occurrence of specific antibodies against HEV among hunters and foresters who are at risk to be exposed. The study group consisted of 210 hunters (23–80 years old) and 60 foresters (22–64 years old). Anti-HEV IgG were present in 3.81% of the samples of the hunters and in 5% of the samples of the foresters. The statistical analysis shows no significant differences inthe results anti-HEV IgG between the groups of hunters and foresters (p = 0.5278). Sign(..)

DOI: 10.5604/01.3001.0010.7882

Published Online: 28-June-2017

Morphological and Molecular Characterization of Phoma complanata, a New Causal Agent of Archangelica officinalis Hoffm. in Poland

Beata Zimowska/ Ewa Dorota Zalewska/ Ewa Dorota Król/ Agnieszka Furmańczyk

The paper concerns the fungus Phoma complanata, isolated for the first time in Poland, from the roots and umbels of angelica (Archangelica officinalis) in 2009. The morphology of fungal isolates was tested on standard culture media. Moreover, the sequence analysis of ITS regions was conducted. Morphological similarity of P. complanata Polish isolates to the reference isolate obtained from CBS culture collection was determined and together with the molecular analysis confirmed the affiliation of (..)

DOI: 10.5604/01.3001.0010.7886

Published Online: 28-June-2017

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