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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology

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ISSN: 1733-1331
eISSN: 2544-4646

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FEATURED ARTICLES

Comparison of PCR, Fluorescent in Situ Hybridization and Blood Cultures for Detection of Bacteremia in Children and Adolescents During Antibiotic Therapy
Epilithic Biofilms in Lake Baikal: Screening and Diversity of PKS and NRPS Genes in the Genomes of Heterotrophic Bacteria
Functional and Transcriptomic Characterization of a Dye-decolorizing Fungus from Taxus Rhizosphere

VOLUME 66 , ISSUE 3 (September 2017) - List of articles

Metallo-Beta-Lactamase Producing Pseudomonas aeruginosa in a Healthcare Setting in Alexandria, Egypt

Amani F. Abaza/ Soraya A. El Shazly/ Heba S.A. Selim/ Gehan S.A. Aly

Pseudomonas aeruginosa has emerged as a major healthcare associated pathogen that creates a serious public health disaster in both develop­ing and developed countries. In this work we aimed at studying the occurrence of metallo-beta-lactamase (MBL) producing P. aeruginosa in a healthcare setting in Alexandria, Egypt. This cross sectional study included 1583 clinical samples that were collected from patients admit­ted to Alexandria University Students’ Hospital. P. aeruginosa isolates were identi(..)

DOI: 10.5604/01.3001.0010.4855

Published Online: 27-September-2017

Polish Physicians’ Attitudes Towards Antibiotic Prescription and Antimicrobial Resistance

Beata Mazińska/ Waleria Hryniewicz

Antimicrobial resistance has been one of the biggest global current issues in medicine and public health. Overuse and imprudent use of antimicrobial agents are recognized as one of the leading causes of antibiotic resistance. The aim of this study was to analyze the attitudesof Polish physicians practicing at the community level towards antibiotics and antimicrobial resistance. The majority of physicians taking part in the survey believed that Polish people overuse antibiotics (98%). Most physic(..)

DOI: 10.5604/01.3001.0010.4856

Published Online: 27-September-2017

Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363

Pushpendra Awadhiya/ Tushar Banerjee/ Shridhar Patil

Delftia acidovorans MTCC 3363 was found to convert 16-dehydropregnenolone acetate (16-DPA) exclusively to 4-androstene-3, 17-dione (AD). Addition of 9α-hydroxylase inhibitors was not required for preventing the accumulation of byproducts. The effect of pH, tempera­ture, substrate concentration, surfactants and carrier solvents on this bioconversion has been studied. 16-DPA was maximally converted in buffered medium at pH 7.0, at temperature 30°C and 0.5 mg ml–1 substrate concentration. Detergent(..)

DOI: 10.5604/01.3001.0010.4858

Published Online: 27-September-2017

Changes in the Concentration of Carbonyl Compounds during the Alcoholic Fermentation Process Carried out with Saccharomyces cerevisiae Yeast

Grzegorz Kłosowski/ Dawid Mikulski/ Aleksandra Rolbiecka/ Bogusław Czupryński

The aim of the study was to determine the influence of the source material and the applied S. cerevisiae strain on the concentrationsof carbonyl fractions in raw spirits. Acetaldehyde was the most common aldehyde found, as it accounted for 88–92% of the total amount of aldehydes. The concentration of acetaldehyde in maize, rye and amaranth mashes was highly correlated with fermentation productivity at a given phase of the process, and reached its highest value of 193.5 mg/l EtOH in the first hou(..)

DOI: 10.5604/01.3001.0010.4861

Published Online: 27-September-2017

The Ability of a Novel Strain Scheffersomyces (Syn. Candida) shehatae Isolated from Rotten Wood to Produce Arabitol

Monika Kordowska-Wiater/ Adam Kuzdraliński/ Tomasz Czernecki/ Zdzisław Targoński/ Magdalena Frąc/ Karolina Oszust

Arabitol is a polyalcohol which has about 70% of the sweetness of sucrose and an energy density of 0.2 kcal/g. Similarly to xylitol, it can be used in the food and pharmaceutical industries as a natural sweetener, a texturing agent, a dental caries reducer, and a humectant. Bio­technological production of arabitol from sugars represents an interesting alternative to chemical production. The yeast Scheffersomyces shehatae strain 20BM-3 isolated from rotten wood was screened for its ability to pro(..)

DOI: 10.5604/01.3001.0010.4863

Published Online: 27-September-2017

Metagenomic Analysis of Soil Bacterial Community and Level of Genes Responsible for Biodegradation of Aromatic Hydrocarbons

Jakub Czarny/ Justyna Staninska-Pięta/ Jolanta Powierska-Czarny/ Jacek Nowak/ Łukasz Wolko/ Agnieszka Piotrowska-Cyplik

The aim of the studies was to compare the composition of soil bacterial metabiomes originating from urbanized areas and areas con­taminated with hydrocarbons with those from agricultural soil and forest soil obtained from a protected wild-life park area. It should be noted that hydrocarbons are everywhere therefore bacteria capable of their utilization are present in every soil type. In the hydrocarbon-contaminated soil and in the soil of anthropogenic origin, the bacteria belonging to Gammaprot(..)

DOI: 10.5604/01.3001.0010.4865

Published Online: 27-September-2017

Comparison of Microbial Communities Associated with Halophyte (Salsola stocksii) and Non-Halophyte (Triticum aestivum) Using Culture-Independent Approache

Salma Mukhtar/ Ayesha Ishaq/ Sara Hassan/ Samina Mehnaz/ Muhammad S. Mirza/ Kauser A. Malik

Halophyte microbiome contributes significantly to plant performance and can provide information regarding complex ecological processes involved in osmoregulation of these plants. The objective of this study is to investigate the microbiomes associated with belowground (rhizo­sphere), internal (endosphere) and aboveground (phyllosphere) tissues of halophyte (Salsola stocksii) through metagenomics approach. Plant samples were collected from Khewra Salt Mines. The metagenomic DNA from soil, root an(..)

DOI: 10.5604/01.3001.0010.4866

Published Online: 27-September-2017

Coomassie Blue G250 for Visualization of Active Bacteria from Lake Environment and Culture

Bartosz Kiersztyn/ Waldemar Siuda/ Ryszard Chróst

Bacteria play a fundamental role in the cycling of nutrients in aquatic environments. A precise distinction between active and inactive bacteria is crucial for the description of this process. We have evaluated the usefulness of Coomassie Blue G250 for fluorescent staining of protein containing potentially highly active bacteria. We found that the G250 solution has excitation and emission properties appropriate for direct epifluorescence microscopy observations. It enables fast and effective flu(..)

DOI: 10.5604/01.3001.0010.4867

Published Online: 27-September-2017

Bacterial Communities from the Arsenic Mine in Złoty Stok, Sudety Mountains, Poland

Tomasz Cłapa/ Dorota Narożna/ Rafał Siuda/ Andrzej Borkowski/ Marek Selwet/ Cezary J. Mądrzak/ Ewa Koźlecka

Investigations of bacterial communities and characterization of mineralogy of the environment in the Złoty Stok As-Au deposit werecarried out. PXRD analysis revealed the presence of picropharmacolite as the most common secondary arsenic mineral in the mine. Total DNA was extracted from slime streams or slime biofilms samples to investigate the bacterial communities. PCR amplification of 16S rDNA was performed followed by subcloning of its products. Over 170 clones were analyzed by means of RFLP (..)

DOI: 10.5604/01.3001.0010.4875

Published Online: 27-September-2017

MRSA in Pig Population

Meldra Ivbule/ EdvĪns Miklaševičs/ Liene Čupāne/ Laima Bērziņa/ Andris Bāliņš/ Anda Valdovska

Methicillin resistant Staphylococcus aureus (MRSA) is widespread worldwide in different types of animal species and as a zoonosis takesa great risk for human health not only as a food toxicoinfection, but also as a highly resistant pathogen causing serious soft tissue infectious, septicaemia and even death. One of the most affected food-producing animal species is swine in the production of which new antibiotics in big amounts are used more and more continuously, increasing antimicrobial resista(..)

DOI: 10.5604/01.3001.0010.4876

Published Online: 27-September-2017

Genes Controlling 2-deoxyglucose Induced Lysis and Formation of Reactive Oxygen Species in Schizosaccharomyces pombe

Akshay Vishwanatha/ Cletus J.M. D’Souza/ Martin E. Schweingruber

Schizosaccharomyces pombe cells of strains each carrying a deletion of one of the genes snf5, ypa1, pho7 and pas1 and of a strain overexpress­ing gene odr1, have been previously shown to grow in presence of the toxic glucose analogue 2-deoxyglucose (2-DG). Here we report that these genes control 2-DG induced lysis and are, with the exception of odr1, also involved in control of formation of reactive oxygen species (ROS) upon exposure of cells to H2O2. Lysis of deletion strains, but not of strain(..)

DOI: 10.5604/01.3001.0010.4877

Published Online: 27-September-2017

Clonal Analysis of Clinical and Environmental Pseudomonas aeruginosa Isolates from Meknes Region, Morocco

Itto Maroui/ Abouddihaj Barguigua/ Asmae Aboulkacem/ Hanane Elhafa/ Khadija Ouarrak/ Mohammed Sbiti/ Lhoussain Louzi/ Mohammed Timinouni/ Abdelhaq Belhaj

From 123 clinical and environmental Pseudomonas aeruginosa isolates, 24 strains were selected for their similar antibioresistance, virulence and biofilm formation profiles, to examine their diversity and occurrence of clones within two hospitals and different natural sites in Meknes (Morocco). Pulsed-field gel electrophoresis, using DraI enzyme, didn’t reveal a close relationship between clinical and environmentalisolates nor between strains of the two hospitals. 19 genotypes were obtained, incl(..)

DOI: 10.5604/01.3001.0010.4882

Published Online: 27-September-2017

KPC-2-producing Klebsiella pneumoniae ST11 in a Children’s Hospital in Poland

Monika Machulska/ Anna Baraniak/ Iwona Żak/ Katarzyna Bojarska/ Dorota Żabicka/ Iwona Sowa-Sierant/ Waleria Hryniewicz/ Marek Gniadkowski

Four Klebsiella pneumoniae isolates from children hospitalized over 10 months in an intensive care unit in a children’s teaching hospital in Poland were analyzed. All of the isolates belonged to a single pulsotype and sequence type (ST) 11, and produced the KPC-2 carbapenemase and extended-spectrum β-lactamase (ESBL) CTX-M-15. They were resistant to a variety of antimicrobials, and their β-lactam resistance patterns were typical for KPC producers. This is one of few cases of identification of KP(..)

DOI: 10.5604/01.3001.0010.4884

Published Online: 27-September-2017

Genetic Characterization of Human Enteroviruses Associated with Hand, Foot and Mouth Diseases in Poland, 2013–2016

Magdalena Wieczorek/ Agnieszka Ciąćka/ Arleta Krzysztoszek/ Agnieszka Figas/ Leszek Szenborn

The objective of the present study was to describe the molecular characteristics of enteroviruses associated with hand, food, and mouth disease (HFMD) in Poland. Clinical material from HFMD cases, that occurred during 2013–2016 were examined. It has been showed that coxsackievirus A6 (CVA6), CVA10 and CVA16 were circulating in the country. Phylogenetic analysis showed that Polish CVA6 strains were divided into two distinct clusters suggesting two independent introductions. This is the first repo(..)

DOI: 10.5604/01.3001.0010.4889

Published Online: 27-September-2017

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