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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology

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ISSN: 1733-1331
eISSN: 2544-4646

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FEATURED ARTICLES

Comparison of PCR, Fluorescent in Situ Hybridization and Blood Cultures for Detection of Bacteremia in Children and Adolescents During Antibiotic Therapy
Epilithic Biofilms in Lake Baikal: Screening and Diversity of PKS and NRPS Genes in the Genomes of Heterotrophic Bacteria
Functional and Transcriptomic Characterization of a Dye-decolorizing Fungus from Taxus Rhizosphere

VOLUME 66 , ISSUE 4 (December 2017) - List of articles

Transferrin and Lactoferrin – Human Iron Sources for Enterococci

Paweł Lisiecki

To overcome limitations in iron acquisition, enterococci have evolved a number of mechanisms to scavenge iron from the host iron-binding proteins – transferrin (TR) and lactoferrin (LF). The aim of this study was to demonstrate the mechanisms by which enterococci utilize human TR and LF bound iron. The study included two strains of Enterococcus faecalis grown in iron-deficient and iron-excess media respectively. The binding activity of both proteins was monitored using proteins labelled with 125(..)

DOI: 10.5604/01.3001.0010.6495

Published Online: 04-December-2017

The Prevalence of Exoenzyme S Gene in Multidrug-Sensitive and Multidrug-Resistant Pseudomonas aeruginosa Clinical Strains

Tomasz Bogiel/ Aleksander Deptuła/ Joanna Kwiecińska-Piróg/ Małgorzata Prażyńska/ Agnieszka Mikucka/ Eugenia Gospodarek-Komkowska

Pseudomonas aeruginosa rods are one of the most commonly isolated microorganisms from clinical specimens, usually responsible for nosocomial infections. Antibiotic-resistant P. aeruginosa strains may present reduced expression of virulence factors. This fact may be caused by appropriate genome management to adapt to changing conditions of the hospital environment. Virulence factors genes maybe replaced by those crucial to survive, like antimicrobial resistance genes. The aim of this study was to(..)

DOI: 10.5604/01.3001.0010.6500

Published Online: 04-December-2017

Temperature, pH and Trimethoprim-Sulfamethoxazole Are Potent Inhibitors of Biofilm Formation by Stenotrophomonas maltophilia Clinical Isolates

Marjan Biočanin/ Haowa Madi/ Zorica Vasiljević/ Milan Kojić/ Branko Jovčić/ Jelena Lozo

Stenotrophomonas maltophilia, an opportunistic pathogen usually connected with healthcare-associated infections, is an environmental bacterium. Intrinsic resistance to multiple antibiotics, with different virulence determinants in the last decade classified this bacterium in the group of global multiple drug resistant (MDR) organism. S. maltophilia clinical isolates, were collected from tertiary care pediatric hospital in Belgrade, Serbia to investigate influence of different factors on biofilm (..)

DOI: 10.5604/01.3001.0010.6996

Published Online: 04-December-2017

Prevalence, Influencing Factors, Antibiotic Resistance, Toxin and Molecular Characteristics of Staphylococcus aureus and MRSA Nasal Carriage among Diabetic Population in the United States, 2001–2004

Jialing Lin/ Yang Peng/ Chan Bai/ Ting Zhang/ Haoqu Zheng/ Xiaojie Wang/ Jiaping Ye/ Xiaohua Ye/ Ying Li/ Zhenjiang Yao

Diabetic population were reported more likely to suffer carriage and infection with Staphylococcus aureus (S. aureus) and methicillin-resistant Staphylococcus aureus (MRSA) than non-diabetic population. We aim to elucidate the prevalence and characteristics of S. aureus and MRSA nasal carriage among diabetic population in the United States National Health and Nutrition Examination Survey, 2001–2004. Univariate analyses were conducted using Chi-square test, Fisher’s exact probability test or stud(..)

DOI: 10.5604/01.3001.0010.7038

Published Online: 04-December-2017

Production, Characterization and Valuable Applications of Exopolysaccharides from Marine Bacillus subtilis SH1

Sahar W.M. Hassan/ Hassan A.H. Ibrahim

Exopolysaccharides (EPSs) are high molecular weight polymers consisting of different sugar residues they are preferable for replacing synthetic polymers as they are degradable and nontoxic. Many microorganisms possess the ability to synthesize and excrete exopolysac­charides with novel chemical compositions, properties and structures to have potential applications in different fields. The present study attempt to optimize the production of EPS by marine Bacillus subtilis SH1 in addition to chara(..)

DOI: 10.5604/01.3001.0010.7001

Published Online: 04-December-2017

Characterisation of Antimicrobial Properties of Extracts of Selected Medicinal Plants

Monika Cioch/ Paweł Satora/ Magdalena Skotniczy/ Dorota Semik-Szczurak/ Tomasz Tarko

The scope of the experiments included analysis of the antimicrobial activity of ethanolic, methanolic and aqueous extracts against bacterial and fungal cultures and determination of the minimum inhibitory concentration of plant extracts tested microbial growth. Analysis of the antifungal and antibacterial activity was carried out by the disc diffusion method using paper discs. In the experiment 11 species of microorganisms – 8 bacterial and 3 fungal strains were used. The highest antimicrobial a(..)

DOI: 10.5604/01.3001.0010.7002

Published Online: 04-December-2017

Characterization of Bacteriocin-Producing Lactic Acid Bacteria Isolated from Native Fruits of Ecuadorian Amazon

Karina Garzón/ Clara Ortega/ Gabriela N. Tenea

Tropical, wild-type fruits are considered biodiverse “hotspots” of microorganisms with possible functional characteristics to be investigated. In this study, several native lactic acid bacteria (LAB) of Ecuadorian Amazon showing highly inhibitory potential were identified and characterized. Based on carbohydrate fermentation profile and 16S rRNA gene sequencing, seven strains were assigned as Lactobacillus plantarum and one strain as Weissella confusa. Using agar-well diffusion method the active(..)

DOI: 10.5604/01.3001.0010.7037

Published Online: 04-December-2017

Physiological Effects of Whey- and Milk-Based Probiotic Yogurt in Rats

Judit Szabó-Fodor/ András Bónai/ Brigitta Bóta/ Linda Szommerné Egyed/ Ferenc Lakatos/ Gréta Pápai/ Attila Zsolnai/ Róbert Glávits/ Katalin Horvatovich/ Melinda Kovács

In an in vitro experiment commercially available probiotic products were tested for the survival of bacteria under conditions of simulated human digestion either when used alone or mixed into yogurt. In the in vivo experiment the effects of feeding a whey- and milk-based yogurt prepared with the probiotic strain showing adequate survival in the in vitro experiment, was measured on body weight, feed con­sumption and immune response of rats (IgG and IgA level after immunisation), on the compositio(..)

DOI: 10.5604/01.3001.0010.7039

Published Online: 04-December-2017

Identification of Lactobacillus delbrueckii and Streptococcus thermophilus Strains Present in Artisanal Raw Cow Milk Cheese Using Real-time PCR and Classic Plate Count Methods

Milena A. Stachelska

The aim of this paper was to detect Lactobacillus delbrueckii and Streptococcus thermophilus using real-time quantitative PCR assay in 7-day ripening cheese produced from unpasteurised milk. Real-time quantitative PCR assays were designed to identify and enumerate the chosen species of lactic acid bacteria (LAB) in ripened cheese. The results of molecular quantification and classic bacterial enumeration showed a high level of similarity proving that DNA extraction was carried out in a proper way(..)

DOI: 10.5604/01.3001.0010.7041

Published Online: 04-December-2017

The Determination and Correlation of Various Virulence Genes, ESBL, Serum Bactericidal Effect and Biofilm Formation of Clinical Isolated Classical Klebsiella pneumoniae and Hypervirulent Klebsiella pneumoniae from Respiratory Tract Infected Patients

Rambha K. Shah/ Zhao H. Ni/ Xiao Y. Sun/ Guo Q. Wang/ Fan Li

Klebsiella pneumoniae strains that are commonly recognized by clinicians and microbiologists are termed as classical K. pneumoniae (cKP). A strain with capsule-associated mucopolysaccharide web is known as hypervirulent K. pneumoniae (hvKP) as it enhances the serum resistant and biofilm production. Aim is to determine and correlate various virulence genes, ESBL, serum bactericidal effect and biofilm formation of clinical isolated cKP and hvKP from respiratory tract infected patients. A total of (..)

DOI: 10.5604/01.3001.0010.7042

Published Online: 04-December-2017

Characterization of Microbial Communities in Acidified, Sulfur Containing Soils

Grzegorz Czerwonka/ Iwona Konieczna/ Paulina Żarnowiec/ Artur Zieliński/ Agnieszka Malinowska-Gniewosz/ Agnieszka Gałuszka/ Zdzisław Migaszewski/ Wiesław Kaca

Over a period of three years, microbial communities in acidified soil with high sulfur content were analyzed. In soil water extracts ureolytic, proteolytic, oxidoreductive, and lipolytic activity were detected. The presented results indicate that the enzymatic activity of soil micro­bial communities varied considerably over time. Isolated 26 (80%) bacterial strains belonged to genus Bacillus sp. and were identified bycultivation and 16S rRNA methods. The commercially available procedures for bac(..)

DOI: 10.5604/01.3001.0010.7043

Published Online: 04-December-2017

Study of Patterns and Markers of Human Immune Deficiency Virus -1 (HIV-1) Progression and Unemployment Rate among Patients from Alexandria, Egypt

Faika M. Ghoneim/ May M. Raouf/ Noha S. Elshaer/ Sarah M. Abdelhamid/ Reem A. Noor Eldeen

Middle East and North Africa (MENA) new HIV cases show the highest increase among all regions in the world. Even though Egypt has a low prevalence among the general population (< 0.02%), a national HIV epidemic occurs in certain population risk groups. The current study was conducted to asses clinical and immunological disease progression; following up viral load (VL) and detecting delta-32 CCR5 genotype polymorphism in selected cases, determining unemployment rate and identify predictors of emp(..)

DOI: 10.5604/01.3001.0010.7044

Published Online: 04-December-2017

Analyses of Plasmids Harbouring Quinolone Resistance Determinants in Enterobacteriaceae Members

Bayri Erac/ Fethiye Ferda Yilmaz/ Ismail Ozturk/ Sabire Sohret Aydemir/ Mine Hosgor-Limoncu

The aim of this study was to explore the plasmid characteristics of eight clinical Enterobacteriaceae strains containing extended broad spec­trum beta-lactamases and plasmid-mediated quinolone resistance. Plasmids were transferred by conjugation or transformation and resist­ance determinants were investigated by PCR. We showed that at least one plasmid harbouring qnrB or qnrS determinant was transferred by conjugation in five isolates. QepA determinant was confirmed to be on a non-conjugative pl(..)

DOI: 10.5604/01.3001.0010.7084

Published Online: 04-December-2017

Inhibition of Multidrug-Resistant Gram-Positive and Gram-Negative Bacteria by a Photoactivated Porphyrin

Moreno Bondi/ Anna Mazzini/ Simona De Niederhäusern/ Ramona Iseppi/ Patrizia Messi

The authors studied the in vitro antibacterial activity of the photo-activated porphyrin meso-tri(N-methyl-pyridyl), mono(N-tetradecyl-pyridyl)porphine (C14) against four multidrug-resistant bacteria: Staphylococcus aureus, Enterococcus faecalis (Gram-positive), Escherichia coli, Pseudomonas aeruginosa (Gram-negative). Using 10 μg/ml of porphyrin and 60 sec irradiation we observed the remarkable suscep­tibility of S. aureus and E. faecalis to treatment while, under the same conditions, E. coli a(..)

DOI: 10.5604/01.3001.0010.7092

Published Online: 04-December-2017

Metagenomic Profiling of the Bacterial Community Changes from Koji to Mash Stage in the Brewing of Soy Sauce

Hongbin Wang/ Quanzeng Wei/ Shuqi Gui/ Yongrui Feng/ Yong Zhang/ Yihan Liu/ Fuping Lu

The improvement of soy sauce fermentation is restricted by the insufficient information on bacterial community. In this study, bacterial communities in the koji and mash stage were compared based on next-generation sequencing technology. A total of 29 genera were identi­fied in the koji stage, while 34 in the mash stage. After koji stage, 7 genera disappeared and 12 new genera appeared in the mash stage. The dominant bacteria were Kurthia, Weissella and Staphylococcus in the koji stage and Staph(..)

DOI: 10.5604/01.3001.0010.7097

Published Online: 04-December-2017

Seroprevalence of Rubella and Cytomegalia in Young Women from Biała Podlaska District

Dorota Plewik/ Małgorzata Tokarska-Rodak/ Justyna Paszkiewicz/ Adam Szepeluk

The aim of this study was to analyze the seroprevalence of rubella and cytomegalia among young women. The study included 175 healthy women from the Biała Podlaska District, aged 16 to 35 years. Anti-rubella and anti-CMV IgG were determined by ELISA. 172 (98.3%) study subjects tested positive for rubella antibodies, 1 (0.6%) was seroindeterminate and 2 (1.1%) were seronegative. CMV antibodies were detected in 119 (68.0%) participants; the series included also 1 (0.6%) seroindeterminate and 55 (31(..)

DOI: 10.5604/01.3001.0010.7103

Published Online: 04-December-2017

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