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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology

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ISSN: 1733-1331
eISSN: 2544-4646

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FEATURED ARTICLES

Comparison of PCR, Fluorescent in Situ Hybridization and Blood Cultures for Detection of Bacteremia in Children and Adolescents During Antibiotic Therapy
Epilithic Biofilms in Lake Baikal: Screening and Diversity of PKS and NRPS Genes in the Genomes of Heterotrophic Bacteria
Functional and Transcriptomic Characterization of a Dye-decolorizing Fungus from Taxus Rhizosphere

VOLUME 67 , ISSUE 1 (March 2018) - List of articles

Detection of Acinetobacter spp. in Blood Cultures by an Improved Fluorescent in Situ Hybridization Assay

Hanieh Asaadi/ Behrouz Naeimi/ Somayyeh Gharibi/ Abdalnaser Khosravi/ Sina Dobaradaran/ Reza Taherkhani/ Saeed Tajbakhsh

Fluorescent in situ hybridization (FISH) allows rapid detection of microorganisms. We aimed (i) to evaluate the sensitivity and specificity of FISH for the detection of Acinetobacter spp. in blood culture specimens and (ii) to test the simultaneous application of two genus-specific probes labeled with the same fluorochrome to increase the fluorescent signal intensity and improve the detection of Acinetobacter spp. Three hundred and twenty blood culture specimens were tested via both the conventi(..)

DOI: 10.5604/01.3001.0011.6137

Published Online: 09-March-2018

Non-invasive Diagnostic of Helicobacter pylori in Stools by Nested-qPCR

María I. Taborda/ Gisela Aquea/ Yenny Nilo/ Karla Salvatierra/ Nicolás López/ Sergio López/ Gustavo Bresky/ Juan A. Madariaga/ Vittorio Zaffiri/ Sergio Häberle/ Giuliano Bernal

The aim of this study was to develop a non-invasive diagnostic test for the detection of Helicobacter pylori in stool samples from digestive symptomatic patients, using a new protocol of nested-qPCR. A total of 143 patients were invited to participate in the study. A gastric biopsy of each patient was collected for Rapid Urease Testing (RUT) and histology by Giemsa stain. A fecal sample for nested-qPCR analysis was also obtained. DNA was extracted from the fecal samples, and conventional PCR fol(..)

DOI: 10.5604/01.3001.0011.5881

Published Online: 09-March-2018

Molecular Characterization of the cry Gene profile of Bacillus thuringiensis Isolated from a Caribbean Region of Colombia

Pedro Fragoso/ Alicia Armijo/ Doris Gómez/ Claudio Gómez/ Marco Bugueño/ Gittith Sánchez/ Juan Venegas

In order to characterize native strains of Bacillus thuringiensis of the Colombian Caribbean with toxic effect against insect vectors, 28 samples of bacteria identified as B. thuringiensis were isolated from different soils and muds around the city of Valledupar. Using a biological test, five isolates of B. thuringiensis showed toxic effect against larvae of Aedes aegypti. PCR methods were used to detect cry1, cry2, cry4B, cry10 and cyt1 genes. Cry1 and cry2 genes were detected in 35.7% and 32.1(..)

DOI: 10.5604/01.3001.0011.6138

Published Online: 09-March-2018

High-Throughput Sequencing Analysis of Endophytic Bacteria Diversity in Fruits of White and Red Pitayas from Three Different Origins

Zhen Ren/ Shukun Tang/ Yi Jiang/ Mingxing Jiang/ Shangyong Zheng/ Wenjing Liu/ Zhili Yang/ Shuping Sang/ Zebin Chen/ Tiyuan Xia/ Min Yin

Pitaya contains various types of polyphenols, flavonoid and vitamins which are beneficial for health and it is among the most important commercial tropical fruits worldwide. Endophytic bacteria might be beneficial for plant growth and yield. However, bacterial diversity in pitaya is poorly characterized. In this study, fruits of white and red pitayas from three different origins (Thailand, Vietnam and China) were chosen for endophytic bacteria diversity investigation by using Illumina HiSeq seco(..)

DOI: 10.5604/01.3001.0011.6139

Published Online: 09-March-2018

Natural Attenuation Potential of Polychlorinated Biphenyl-Polluted Marine Sediments

Sarah Aldhafiri/ Huda Mahmoud/ Mohammed Al-Sarawi/ Wael A. Ismail

The marine environment in Kuwait is polluted with various hazardous chemicals of industrial origin. These include petroleum hydrocarbons, halogenated compounds and heavy metals. Bioremediation with dedicated microorganisms can be effectively applied for reclamation of the polluted marine sediments. However, information on the autochthonous microbes and their ecophysiology is largely lacking. We analyzed sediments from Shuwaikh harbor to detect polychlorinated biphenyls (PCBs) and total petroleum(..)

DOI: 10.5604/01.3001.0011.6140

Published Online: 09-March-2018

Isolation and Characteristics of Biotechnologically Important Antagonistic Thermophilic Bacteria from Rhizosphere of Haloxylon salicornicum

Muhammad Aslam/ Faiz-Ul-Hassan Nasim/ Rana Ruhi/ Hassan Murad/ Samina Ejaz/ Muhammad Shafiq Choudhary/ Ghulam Mustafa/ Muhammad Ashraf/ Jameel Rehman

Rhizobacteria are an active part of microbial population in the rhizosphere of plants. In this study, twenty rhizobacteria were isolated from the rhizosphere of a perennial grass, Haloxylon salicornicum, found in Cholistan desert, an arid landmass near Bahawalpur Pakistan, in one set of experimental conditions. Colony characteristics, biochemical and molecular analyses of these isolates were performed. All isolates were bacilli, gram positive with off-white colonies and exhibited typical bacilli(..)

DOI: 10.5604/01.3001.0011.6142

Published Online: 09-March-2018

A Low-Tech Bioreactor System for the Enrichment and Production of Ureolytic Microbes

Masataka Aoki/ Takuya Noma/ Hiroshi Yonemitsu/ Nobuo Araki/ Takashi Yamaguchi/ Kazuyuki Hayashi

Ureolysis-driven microbially induced carbonate precipitation (MICP) has recently received attention for its potential biotechnological applications. However, information on the enrichment and production of ureolytic microbes by using bioreactor systems is limited. Here, we report a low-tech down-flow hanging sponge (DHS) bioreactor system for the enrichment and production of ureolytic microbes. Using this bioreactor system and a yeast extract-based medium containing 0.17 M urea, ureolytic microb(..)

DOI: 10.5604/01.3001.0011.6144

Published Online: 09-March-2018

MALDI-TOF MS Detection of Endophytic Bacteria Associated with Great Nettle (Urtica dioica L.), Grown in Algeria

Souheyla Toubal/ Ouahiba Bouchenak/ Djillali Elhaddad/ Karima Yahiaoui/ Sarah Boumaza/ Karim Arab

Any plant with a vascular system has a specific endophytic microflora. The identification of bacteria is essential in plant pathology. Although identification methods are effective, they are costly and time consuming. The purpose of this work is to isolate and to identify the different bacteria from the internal tissues of Urtica dioica L. and to study their diversity. This last is based on the different parts of the plant (stems, leaves and roots) and the harvest regions (Dellys and Tlamcen). T(..)

DOI: 10.5604/01.3001.0011.6145

Published Online: 09-March-2018

The Very Low Frequency of Epstein-Barr JC and BK Viruses DNA in Colorectal Cancer Tissues in Shiraz, Southwest Iran

Jamal Sarvari/ Shahab Mahmoudvand/ Neda Pirbonyeh/ Akbar Safaei/ Seyed Younes Hosseini

Viruses including Epstein-Barr virus (EBV), JCV and BKV have been reported to be associated with some cancers. The association of these viruses with colorectal cancers remains controversial. Our objective was to investigate their infections association with adenocarcinoma and adenomatous polyps of the colon. Totally, 210 paraffin-embedded tissue specimens encompassing 70 colorectal adenocarcinoma, 70 colorectal adenomatous and 70 colorectal normal tissues were included. The total DNA was extract(..)

DOI: 10.5604/01.3001.0011.6146

Published Online: 09-March-2018

Comparison of Methods Used for the Diagnosis of Epstein-Barr Virus Infections in Children

Nilgun Kasifoglu/ Semra Oz/ Ener Cagri Dinleyici/ Tercan Us/ Ozcan Bor/ Gul Durmaz/ Yurdanur Akgun

The accurate diagnosis of Epstein-Barr virus (EBV) infections is important, as many other infectious agents or diseases can cause similar symptoms. In this study, sera of pediatric patients who were suspected to have an EBV infection, were sent to Eskisehir Osmangazi University Faculty of Medicine, Department of Clinical Microbiology, and investigated by IFA, ELISA, immunoblotting and Real-time PCR. The performances of these tests were compared with IFA. The rates of agreement between ELISA and (..)

DOI: 10.5604/01.3001.0010.6287

Published Online: 09-March-2018

Isolation of Sabin-like Polioviruses from Sewage in Poland

Agnieszka Figas/ Magdalena Wieczorek/ Anna Żuk-Wasek/ Bogumiła Litwińska

As a complement to the active search for cases of acute flaccid paralysis, environmental sampling was conducted from January to December 2011, to test for any putative polio revertants and recombinants in sewage. A total of 165 environmental samples were obtained and analyzed for the presence of polioviruses by use of cell culture (L20B, RD and Caco-2) followed by neutralization and reverse-transcription polymerase chain reaction. Out of the 31 CPE positive samples, 26 contained one and 5 two di(..)

DOI: 10.5604/01.3001.0011.6147

Published Online: 09-March-2018

Evaluation of the Carba NP Test for the Detection of Carbapenemase Activity in Bacteroides Species

Isin Akyar/ Meltem Ayas/ Onur Karatuna/ Yesim Besli

We evaluated the usefulness of the Carba NP test for rapid detection of carbapenemase activity in Bacteroides spp. The minimum inhibitory concentration (MIC) for imipenem was determined with gradient test strips, and cfiA gene was investigated by polymerase chain reaction for 27 clinical Bacteroides spp. isolates. Carba NP test was performed according to recommendations of the Clinical and Laboratory Standards Institute. Among three cfiA gene harboring clinical isolates, two imipenem resistant i(..)

DOI: 10.5604/01.3001.0011.6148

Published Online: 09-March-2018

Rapid Detection of Bloodstream Pathogens in Oncologic Patients with a FilmArray Multiplex PCR Assay: a Comparison with Culture Methods

Maria Szymankiewicz/ Beata Nakonowska

The results of the FilmArray® Blood Culture Identification Panel (BCID) (BioFire Diagnostics) and the culture with susceptibility testing of 70 positive blood cultures from oncologic patients were compared. The multiplex PCR assay (BCID) identified 81 of the 83 isolates (97.6%), covered by the panel. The panel produced results in significantly shorter time than standard identification methods, when counted from receiving positive blood cultures bottles to the final results. It is an accurate met(..)

DOI: 10.5604/01.3001.0011.6149

Published Online: 09-March-2018

Changes of Microbial Diversity During Swine Manure Treatment Process

Minseok Kim/ Jung-Im Yun/ Seung-Gun Won/ Kyu-Hyun Park

We investigated microbial diversity in a manure storage tank (MST) storing untreated manure and an aeration tank (AT) during swine manure treatment process using the next-generation sequencing in order to find the aeration effect on microbial diversity. Proteobacteria were more abundant in the AT group than in the MST group and may include denitrifying bacteria contributing to nitrous oxide (N2O) emission or aerobic bacteria stimulated by oxygen. The opposite held true for the phyla Bacteroidete(..)

DOI: 10.5604/01.3001.0011.6150

Published Online: 09-March-2018

Comparative Seroprevalence of Hepatitis A And E Viruses in Blood Donors from Wielkopolska Region, West-Central Poland

Maciej Bura/ Małgorzata Łagiedo-Żelazowska/ Michał Michalak/ Jan Sikora/ Iwona Mozer-Lisewska

The objective of the present study was to investigate the seroprevalence of HAV and HEV in Polish blood donors (BDs). One hundred and ten randomly selected healthy BDs, living in Wielkopolska Region were tested for anti-HAV IgG and anti-HEV IgG with commercial assays. The seroprevalence of anti-HAV was 11.8%; anti-HEV were detected in 60.9% of BDs (p < 0.0001). Consumption of risky food was more common in anti-HEV-positive BDs (59.1% vs. 33.3%; p = 0.01). Twelve out of 20 BDs (60%) with no histo(..)

DOI: 10.5604/01.3001.0011.6151

Published Online: 09-March-2018

The Prevalence of Campylobacter spp. in Polish Poultry Meat

Anna Szosland-Fałtyn/ Beata Bartodziejska/ Joanna Królasik/ Beata Paziak-Domańska/ Dorota Korsak/ Magdalena Chmiela

The prevalence, count and molecular identification of Campylobacter spp. in Polish poultry meat were analysed. 181 samples of meat from chicken (70), turkey (47), duck (54) and goose (10) were studied. Campylobacter spp. was found in 64% of meat samples. The highest prevalence of this pathogen was detected for duck meat. On average 80% of duck samples were contaminated with Campylobacter spp. The counts of Campylobacter spp. in positive samples remained under ten colony forming units per gram of(..)

DOI: 10.5604/01.3001.0011.6152

Published Online: 09-March-2018

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