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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology

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ISSN: 1733-1331
eISSN: 2544-4646

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FEATURED ARTICLES

Differentially Marked IncP-1β R751 Plasmids for Cloning via Recombineering and Conjugation
Colistin Resistance in Enterobacterales Strains – A Current View
Antibiotic Resistance Among Uropathogenic Escherichia coli

VOLUME 68 , ISSUE 4 (Dec 2019) - List of articles

Antibiotic Resistance Among Uropathogenic Escherichia coli

BARBARA KOT

Abstract Urinary tract infections (UTIs) belong to the most common community-acquired and nosocomial infections. A main etiological factor of UTIs is uropathogenic Escherichia coli (UPEC). This review describes the current state of knowledge on the resistance of UPEC to antibiotics recommended for the treatment of UTIs based on the available literature data. Nitrofurantoin and fosfomycin are recommended as first-line therapy in the treatment of uncomplicated cystitis, and the resistance to these(..)

DOI: 10.33073/pjm-2019-048

Colistin Resistance in Enterobacterales Strains – A Current View

ELŻBIETA M. STEFANIUK/ STEFAN TYSKI

Abstract Colistin is a member of cationic polypeptide antibiotics known as polymyxins. It is widely used in animal husbandry, plant cultivation, animal and human medicine and is increasingly used as one of the last available treatment options for patients with severe infections with carbapenem-resistant Gram-negative bacilli. Due to the increased use of colistin in treating infections caused by multidrug-resistant (MDR) bacteria, the resistance to this antibiotic ought to be monitored. Bacterial(..)

DOI: 10.33073/pjm-2019-055

Molecular Identification of Vibrio alginolyticus Causing Vibriosis in Shrimp and Its Herbal Remedy

MD. ABDUL HANNAN/ MD. MAHBUBUR RAHMAN/ MD. NURUNNABI MONDAL/ DEB SUZAN CHANDRA/ GAZLIMA CHOWDHURY/ MD. TOFAZZAL ISLAM

Abstract Penaeus monodon is highly susceptible to vibriosis disease. Aims of the study were to identify the pathogen causing vibriosis in P. monodon through molecular techniques and develop a biocontrol method of the disease by application of herbal extracts. Shrimp samples were collected aseptically from the infected farm and the bacteria were isolated from the infected region of those samples. Based on phenotypic identification, several isolates were identified as Vibrio sp. 16S rRNA gene sequ(..)

DOI: 10.33073/pjm-2019-042

Salmonella-Infected Aortic Aneurysm: Investigating Pathogenesis Using Salmonella Serotypes

CHISHIH CHU/ MIN YI WONG/ CHENG-HSUN CHIU/ YUAN-HSI TSENG/ CHYI-LIANG CHEN/ YAO-KUANG HUANG

Abstract Salmonella infection is most common in patients with infected aortic aneurysm, especially in Asia. When the aortic wall is heavily atherosclerotic, the intima is vulnerable to invasion by Salmonella, leading to the development of infected aortic aneurysm. By using THP-1 macrophage-derived foam cells to mimic atherosclerosis, we investigated the role of three Salmonella enterica serotypes – Typhimurium, Enteritidis, and Choleraesuis – in foam cell autophagy and inflammasome formation. He(..)

DOI: 10.33073/pjm-2019-043

Molecular Epidemiology of Hepatitis B Virus in Turkish Cypriot

UNAL SUMER/ MURAT SAYAN

Abstract There is an increased demand for molecular and epidemiological information regarding Hepatitis B Virus (HBV) infection as the disease severity depends on these specifications. We have aimed to analyze nucleos(t)ide analogues (NA) resistance and typical HBsAg escape mutations with the dispersion of HBV genotype/subgenotype/HBsAg serotypes on overlapping pol/S gene regions in the Turkish population. Samples were collected in Northern Cyprus. Reverse transcriptase (rt) region between 80–25(..)

DOI: 10.33073/pjm-2019-044

Cytokine Levels in the In Vitro Response of T Cells to Planktonic and Biofilm Corynebacterium amycolatum

ALINA OLENDER/ AGNIESZKA BOGUT/ AGNIESZKA MAGRYŚ/ JACEK TABARKIEWICZ

Abstract Unravelling of the interplay between the immune system and non-diphtheria corynebacteria would contribute to understanding their increasing role as medically important microorganisms. We aimed at the analysis of pro- (TNF, IL-1β, IL-6, IL-8, and IL-12p70) and anti-inflammatory (IL-10) cytokines produced by Jurkat T cells in response to planktonic and biofilm Corynebacterium amycolatum. Two reference strains: C. amycolatum ATCC 700207 (R-CA), Staphylococcus aureus ATCC 25923 (R-SA), and (..)

DOI: 10.33073/pjm-2019-045

The Diversity of the Endobiotic Bacterial Communities in the Four Jellyfish Species

QING LIU/ XINTONG CHEN/ XIAOYA LI/ JIANPING HONG/ GUIXIAN JIANG/ HONGYU LIANG/ WENWEN LIU/ ZHENG XU/ JING ZHANG/ WEI WANG/ LIANG XIAO

Abstract The associated microbiota plays an essential role in the life process of jellyfish. The endobiotic bacterial communities from four common jellyfish Phyllorhiza punctata, Cyanea capillata, Chrysaora melanaster, and Aurelia coerulea were comparatively analyzed by 16S rDNA sequencing in this study. Several 1049 OTUs were harvested from a total of 130 183 reads. Tenericutes (68.4%) and Firmicutes (82.1%) are the most abundant phyla in P. punctata and C. melanaster, whereas C. capillata and (..)

DOI: 10.33073/pjm-2019-046

Inhibition of Drug Resistance of Staphylococcus aureus by Efflux Pump Inhibitor and Autolysis Inducer to Strengthen the Antibacterial Activity of β-lactam Drugs

WENJING LUAN/ XIAOLEI LIU/ XUEFEI WANG/ YANAN AN/ YANG WANG/ CHAO WANG/ KESHU SHEN/ HONGYUE XU/ SHULIN LI/ MINGYUAN LIU/ LU YU

Abstract This study explored a potential treatment against methicillin-resistant Staphylococcus aureus (MRSA) infections that combines thioridazine (TZ), an efflux pump inhibitor, and miconazole (MCZ), an autolysis inducer, with the anti-microbial drug cloxacillin (CXN). In vitro, the combination treatment of TZ and MCZ significantly reduced 4096-fold (Σ (FIC) = 0.1 – 1.25) the MIC value of CXN against S. aureus. In vivo, the combination therapy significantly relieved breast redness and swelling(..)

DOI: 10.33073/pjm-2019-047

Diversity, Virulence Factors, and Antifungal Susceptibility Patterns of Pathogenic and Opportunistic Yeast Species in Rock Pigeon (Columba livia) Fecal Droppings in Western Saudi Arabia

HUSSEIN H. ABULREESH/ SAMEER R. ORGANJI/ KHALED ELBANNA/ GAMAL E.H. OSMAN/ MESHAL H.K. ALMALKI/ AHMED Y. ABDEL-MALEK/ ABDULLAH A.K. GHYATHUDDIN/ IQBAL AHMAD

Abstract Bird fecal matter is considered a potential source of pathogenic microbes such as yeast species that contaminate the environment. Therefore, it needs to be scrutinized to assess potential environmental health risks. The aim of this study was to investigate the diversity of the yeasts in pigeon fecal droppings, their antifungal susceptibility patterns, and virulence factors. We used culturing techniques to detect the yeasts in pigeon fecal droppings. The isolates were then characterized (..)

DOI: 10.33073/pjm-2019-049

The Composition of Fungal Communities in the Rumen of Gayals (Bos frontalis), Yaks (Bos grunniens), and Yunnan and Tibetan Yellow Cattle (Bos taurs)

HOUFU WANG/ PENGFEI LI/ XUCHUAN LIU/ CHUNYONG ZHANG/ QIONGFEN LU/ DONGMEI XI/ RENHUI YANG/ SHULING WANG/ WENSHUN BAI/ ZHEN YANG/ RONGKANG ZHOU/ XIAO CHENG/ JING LENG

Abstract The rumen is a microbial-rich ecosystem in which rumen fungi play an important role in the feed digestion of ruminants. The composition of rumen fungi in free-range ruminants such as gayals, yaks, Tibetan yellow cattle, and the domesticated Yunnan yellow cattle was investigated by sequencing an internal transcribed spacer region 1 (ITS1) using Illumina MiSeq. A total of 285 092 optimized sequences and 904 operational taxonomic units (OTUs) were obtained from the four cattle breeds. The (..)

DOI: 10.33073/pjm-2019-050

New Look on Antifungal Activity of Silver Nanoparticles (AgNPs)

BARBARA ŻAROWSKA/ TOMASZ KOŹLECKI/ MICHAŁ PIEGZA/ KATARZYNA JAROS-KOŹLECKA/ MAŁGORZATA ROBAK

Abstract The progress of research on silver nanoparticles (AgNPs) has led to their inclusion in many consumer products (chemicals, cosmetics, clothing, water filters, and medical devices) as a biocide. Despite the widespread use of AgNPs, their biocidal activity is not yet fully understood and is usually associated with various factors (size, composition, surface, red-ox potential, and concentration) and, obviously, specific features of microorganisms. There are merely a few studies concerning t(..)

DOI: 10.33073/pjm-2019-051

Illumina MiSeq Analysis and Comparison of Freshwater Microalgal Communities on Ulleungdo and Dokdo Islands

HYUN-SIK YUN/ YOUNG-SAENG KIM/ HO-SUNG YOON

Abstract Ulleungdo and Dokdo are volcanic islands with an oceanic climate located off the eastern coast of South Korea. In the present study, we used barcoded Illumina MiSeq to analyze eukaryotic microalgal genera collected from Seonginbong, the highest peak on Ulleungdo, and from groundwater sites on Dongdo and Seodo Islands, which are part of Dokdo. Species richness was significantly greater in the Seonginbong samples than in the Dongdo and Seodo samples, with 834 operational taxonomic units ((..)

DOI: 10.33073/pjm-2019-053

Epidemiology, Drug Resistance, and Virulence of Staphylococcus aureus Isolated from Ocular Infections in Polish Patients

MARTA KŁOS/ MONIKA POMORSKA-WESOŁOWSKA/ DOROTA ROMANISZYN/ AGNIESZKA CHMIELARCZYK/ JADWIGA WÓJKOWSKA-MACH

Abstract Analysis of the epidemiology of Staphylococcus aureus (SA) ocular infections and virulence factors of the isolates with a special emphasis on their drug resistance, and the ability of biofilm formation. In a period from 2009 to 2013, 83 isolates of SA were prospectively collected and preserved in a multicenter laboratory-based study carried out in southern Poland. Epidemiological, phenotypic, and genotypic analyses were performed. The resistance and virulence genes were analyzed. Screen(..)

DOI: 10.33073/pjm-2019-056

Structural Changes of Bacillus subtilis Biomass on Biosorption of Iron (II) from Aqueous Solutions: Isotherm and Kinetic Studies

SRI LAKSHMI RAMYA KRISHNA KANAMARLAPUDI/ SUDHAMANI MUDDADA

Abstract Various microbial biomasses have been employed as biosorbents. Bacterial biomass has added advantages because of easy in production at a low cost. The study investigated the biosorption of iron from aqueous solutions by Bacillus subtilis. An optimum biosorption capacity of 7.25 mg of the metal per gram of the biosorbent was obtained by the Inductive Coupled Plasma Optical Emission Spectroscopy (ICP-OES) under the experimental conditions of initial metal concentration of 100 mg/l, pH 4.5(..)

DOI: 10.33073/pjm-2019-057

Differentially Marked IncP-1β R751 Plasmids for Cloning via Recombineering and Conjugation

ASHVEEN BAINS/ JAMES W. WILSON

Abstract We demonstrate here for the first time the use of an IncP-1β plasmid, R751, as a gene capture vehicle for recombineering/conjugation strategies to clone large segments of bacterial genomes (20 – 100 + Kb). We designed R751 derivatives containing alternative markers for greater flexibility when using the R751 vehicle across different bacteria. These markers are removable if desired as part of the cloning procedure (with no extra steps needed). We demonstrated utility via cloning of 38 an(..)

DOI: 10.33073/pjm-2019-052

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