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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology

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ISSN: 1733-1331
eISSN: 2544-4646

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FEATURED ARTICLES

Differentially Marked IncP-1β R751 Plasmids for Cloning via Recombineering and Conjugation
Differentially Marked IncP-1β R751 Plasmids for Cloning via Recombineering and Conjugation
Colistin Resistance in Enterobacterales Strains – A Current View
Colistin Resistance in Enterobacterales Strains – A Current View
Antibiotic Resistance Among Uropathogenic Escherichia coli
Antibiotic Resistance Among Uropathogenic Escherichia coli

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Volume 69 (2020)
Volume 68 (2019)
Volume 67 (2018)
Volume 66 (2017)
Volume 65 (2016)
Trending Articles
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  4. Antibiotic Susceptibility of Cronobacter spp. Isolated from Clinical Samples
  5. Antibiotic Resistance Among Uropathogenic Escherichia coli

VOLUME 69 , ISSUE 2 (Jun 2020) - List of articles

Prevalence of Multi-Drug Resistant Mycobacterium Tuberculosis in Khyber Pakhtunkhwa – A High Tuberculosis Endemic Area of Pakistan

SAJID ALI/ MUHAMMAD TAHIR KHAN/ ANWAR SHEED KHAN/ NOOR MOHAMMAD/ MUHAMMAD MUMTAZ KHAN/ SAJJAD AHMAD/ SADIQ NOOR/ ABDUL JABBAR/ CANTILLON DAIRE/ FARIHA HASSAN

Abstract Anti-tuberculosis therapy involves the combination of drugs to hamper the growth of Mycobacterium tuberculosis (MTB). The emergence of multidrug-resistant tuberculosis (MDR-TB) is a global concern. Pakistan has been ranked 5th position in terms of a high burden of MDR-TB in the world. The aim of the current study was to investigate the prevalence of drug resistance in MTB in Khyber Pakhtunkhwa. Random samples were collected from 25 districts using the simple random sampling formula. All(..)

DOI: 10.33073/pjm-2020-005

Published Online: 06-April-2020

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PCR-based Screening Approach: A Rapid Method to Detect the Biosynthetic Potential of Antimicrobials in Actinobacterial Strains

NAILA NOUREEN/ MOHSIN TASSAWAR CHEEMA/ SUMAIRA ANWAR/ SHAHIDA HASNAIN/ IMRAN SAJID

Abstract This study aimed to investigate the PCR-based screening strategy for the prediction of the antimicrobial biosynthetic potential of the selected Streptomyces strains originated from an extreme environment (Cholistan Desert, Pakistan). The biosynthetic potential was determined by using both molecular and culture-dependent screening approaches. The four biosynthetic genes clusters, including the pks-1, nrps, cyp P450 hydroxylase (cyps), and glycopeptide oxy b genes, were investigated in th(..)

DOI: 10.33073/pjm-2020-016

Published Online: 12-May-2020

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Characterization of Microbial Diversity and Community Structure in Fermentation Pit Mud of Different Ages for Production of Strong-Aroma Baijiu

XU-JIA WANG/ HONG-MEI ZHU/ ZHI-QIANG REN/ ZHI-GUO HUANG/ CHUN-HUI WEI/ JIE DENG

Abstract In the traditional fermentation process of strong-aroma Baijiu, a fermentation pit mud (FPM) provides many genera of microorganisms for fermentation. However, the functional microorganisms that have an important effect on the quality of Baijiu and their changes with the age of fermentation pit (FP) are poorly understood. Herein, the Roche 454 pyrosequencing technique and a phospholipid fatty-acid analysis were employed to reveal the structure and diversity of prokaryotic communities in (..)

DOI: 10.33073/pjm-2020-018

Published Online: 12-May-2020

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Comparison of Rapid and Routine Methods of Identification and Antibiotic Susceptibility Testing of Microorganisms from Blood Culture Bottles

SADIK AKGUN/ HAKAN SEZGIN SAYINER

Abstract Reporting of the results of routine laboratory blood culture tests to clinicians is vital to the patients’ early treatment. This study aimed to perform identification and antibiotic susceptibility tests of the blood cultures showing positive signals of microbial growth in the first 12 hours of incubation by using centrifugation and Gram staining of 5 ml of liquid from the vial, thus achieving faster results. This study included 152 consecutively incubated blood culture samples showing p(..)

DOI: 10.33073/pjm-2020-019

Published Online: 15-May-2020

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The Resurgence of Measles Infection and its Associated Complications in Early Childhood at a Tertiary Care Hospital in Peshawar, Pakistan

MUHAMMAD ILYAS/ SUMERA AFZAL/ JAMSHAID AHMAD/ SAAD ALGHAMDI/ MUHAMMAD KHURRAM

Abstract Measles infection is of substantial interest to immunologists due to its paradoxical interaction with the immune system. After the acquisition of the measles infection, secondary infection plays a pivotal role in measles-related deaths. A cross-sectional study conducted between December 2018 and July 2019 is presented here. A total of one hundred children of both genders presented with measles complications were included following WHO criteria. Measles confirmation was done by quantitat(..)

DOI: 10.33073/pjm-2020-020

Published Online: 15-May-2020

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A Current Microbiological Picture of Mycobacterium Isolates from Istanbul, Turkey

BILGE SUMBUL/ MEHMET ZIYA DOYMAZ

Abstract Despite advances in diagnosis and treatment, tuberculosis (TB) continues to be one of the essential health problems throughout the world. Turkey is considered to be endemic for TB. In this study, we analyzed the distribution of Mycobacterium species, compare the diagnostic methods, and susceptibilities to anti-tuberculosis drugs of TB isolates. The aim was to document the current status and to provide a frame of reference for future studies. In this study, 278 Mycobacterium species isol(..)

DOI: 10.33073/pjm-2020-021

Published Online: 29-May-2020

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Luffa cylindrica Immobilized with Aspergillus terreus QMS-1: an Efficient and Cost-Effective Strategy for the Removal of Congo Red using Stirred Tank Reactor

QANDEEL LARAIB/ MARYAM SHAFIQUE/ NUSRAT JABEEN/ SEHAR AFSHAN NAZ/ HAFIZ RUB NAWAZ/ BARKAT SOLANGI/ ARIF ZUBAIR/ MUHAMMAD SOHAIL

Abstract Microbial populations within the rhizosphere have been considered as prosperous repositories with respect to bioremediation aptitude. Among various environmental contaminants, effluent from textile industries holds a huge amount of noxious colored materials having high chemical oxygen demand concentrations causing ecological disturbances. The study was aimed to explore the promising mycobiome of rhizospheric soil for the degradation of azo dyes to develop an efficient system for the exc(..)

DOI: 10.33073/pjm-2020-022

Published Online: 04-June-2020

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Lactobacillus fermentum JX306 Restrain D-galactose-induced Oxidative Stress of Mice through its Antioxidant Activity

DI ZHANG/ CHUANG LI/ RUIRUI SHI/ FENGCHUN ZHAO/ ZHENGYOU YANG

Abstract Oxidative stress-induced series of related degenerative diseases have received widespread attention. To screen new lactic acid bacteria (LAB) strains to resist oxidative stress, traditional Chinese fermented vegetables were used as a resource library to screen of LAB. The Lactobacillus fermentum JX306 strain, which showed high scavenging activity of DPPH free radical and hydrogen radical, and a strong lipid peroxidation inhibition rate in vitro was selected. L. fermentum JX306 was also (..)

DOI: 10.33073/pjm-2020-024

Published Online: 04-June-2020

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Performance Evaluation of Different Commercial Serological Kits for Diagnosis of Acute Hepatitis E Viral Infection

QIANG ZHANG/ XIAOLONG ZONG/ DONGMING LI/ JING LIN/ LIHUA LI

Abstract Clinical diagnosis of hepatitis E viral (HEV) infection mainly relies on serological assays, and the current status of misdiagnoses regarding HEV infection is uncertain. In this study, patients with acute HEV infection were tested for anti-HEV IgM and IgG, a HEV antigen (Ag), and viral loads (HEV RNA). Serology was performed using four commercial HEV ELISA kits: Wantai, Kehua, Lizhu, and Genelabs IgM and IgG. The HEV RNA was detected using RT-PCR assays. The sensitivities of different k(..)

DOI: 10.33073/pjm-2020-025

Published Online: 04-June-2020

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N-acetylcysteine (NAC) Attenuating Apoptosis and Autophagy in RAW264.7 Cells in Response to Incubation with Mycolic Acid from Bovine Mycobacterium tuberculosis Complex

XUE LIN/ MENGMENG WEI/ FUYANG SONG/ DI XUE/ YUJIONG WANG

Abstract Bovine tuberculosis is an airborne infectious disease caused by organisms of the Mycobacterium tuberculosis (MTB) complex. Mycolic acid (MA) is the main lipid component of the cell membrane of MTB. It is non-enzymatically reduced by NAD(P)H and further produces reactive oxygen species (ROS), which can cause oxidative stress in human cells. N-acetylcysteine (NAC) is a synthetic precursor of glutathione (GSH) and exhibits anti-ROS activity. However, the underlying mechanisms of its protec(..)

DOI: 10.33073/pjm-2020-026

Published Online: 04-June-2020

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Extensively Drug-resistant Acinetobacter baumannii Belonging to International Clone II from A Pet Cat with Urinary Tract Infection; The First Report from Pakistan

ZEESHAN TAJ/ MUHAMMAD HIDAYAT RASOOL/ AHMAD ALMATROUDI/ MUHAMMAD SAQALEIN/ MOHSIN KHURSHID

Abstract The carbapenem-resistant Acinetobacter baumannii (CRAB) has got global attention as a notorious nosocomial pathogen. This study describes a case of urinary tract infection in a 2-years old pet female cat infected with A. baumannii. The susceptibility profiling, screening for the resistance determinants, and the multilocus sequence typing was performed. The A. baumannii isolate was found to harbor the blaOXA23-like gene and corresponded to International clone II that has been widely repo(..)

DOI: 10.33073/pjm-2020-017

Published Online: 28-April-2020

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Effect of Chlorella vulgaris on Growth and Photosynthetic Pigment Content in Swiss Chard (Beta vulgaris L. subsp. cicla)

TIMEA HAJNAL-JAFARI/ VLADIMIRA SEMAN/ DRAGANA STAMENOV/ SIMONIDA ĐURIĆ

Abstract Microalgae application in agriculture is an alternative measure that could be highly beneficial to plants. The application of microalgae Chlorella vulgaris S45 and its effect on plant growth and pigment content in Swiss chard were investigated. In the treatments, 5% and 10% algal suspensions were applied by spraying on plants and in soil, respectively. C. vulgaris S45 affected the initial growth of Swiss chard and the content of photosynthetic pigments positively. The correlation analys(..)

DOI: 10.33073/pjm-2020-023

Published Online: 29-May-2020

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