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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology

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ISSN: 1733-1331
eISSN: 2544-4646

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FEATURED ARTICLES

Differentially Marked IncP-1β R751 Plasmids for Cloning via Recombineering and Conjugation
Colistin Resistance in Enterobacterales Strains – A Current View
Antibiotic Resistance Among Uropathogenic Escherichia coli

VOLUME 69 , ISSUE 4 (Dec 2020) - List of articles

Hepatitis B Virus: From Diagnosis to Treatment

MERYEM GUVENIR/ AYSE ARIKAN

Abstract Hepatitis B infection is still a global concern progressing as acute-chronic hepatitis, severe liver failure, and death. The infection is most widely transmitted from the infected mother to a child, with infected blood and body fluids. Pregnant women, adolescents, and all adults at high risk of chronic infection are recommended to be screened for hepatitis B infection. The initial analysis includes serological tests that allow differentiation of acute and chronic hepatitis. Molecular as(..)

DOI: 10.33073/pjm-2020-044

Published Online: 27-December-2020

Biofloc Technology: Emerging Microbial Biotechnology for the Improvement of Aquaculture Productivity

MAMDOH T. JAMAL/ MOHAMMED BROOM/ BANDAR A. AL-MUR/ MAMDOUH AL HARBI/ MOHAMMED GHANDOURAH/ AHMED AL OTAIBI/ MD FAZLUL HAQUE

Abstract With the significant increases in the human population, global aquaculture has undergone a great increase during the last decade. The management of optimum conditions for fish production, which are entirely based on the physicochemical and biological qualities of water, plays a vital role in the prompt aquaculture growth. Therefore, focusing on research that highlights the understanding of water quality and breeding systems’ stability is very important. The biofloc technology (BFT) is a(..)

DOI: 10.33073/pjm-2020-049

Published Online: 27-December-2020

Biofilm-Forming Ability and Effect of Sanitation Agents on Biofilm-Control of Thermophile Geobacillus sp. D413 and Geobacillus toebii E134

TUGBA KİLİC

Abstract Geobacillus sp. D413 and Geobacillus toebii E134 are aerobic, non-pathogenic, endospore-forming, obligately thermophilic bacilli. Gram-positive thermophilic bacilli can produce heat-resistant spores. The bacteria are indicator organisms for assessing the manufacturing process’s hygiene and are capable of forming biofilms on surfaces used in industrial sectors. The present study aimed to determine the biofilm-forming properties of Geobacillus isolates and how to eliminate this formation (..)

DOI: 10.33073/pjm-2020-042

Published Online: 23-November-2020

Application of PCR for Specific Diagnosis of Leptospirosis in Humans in Ukraine

OLENA ZUBACH/ OKSANA SEMENYSHYN/ LILYA VASIUYNETS/ OKSANA VELYCHKO/ ALEXANDER ZINCHUK

Abstract Leptospirosis remains one of the most widespread zoonotic diseases in the world and Ukraine, in particular. Ukrainian clinicians have been faced with early detection of the disease due to the availability of only a serological method for routine diagnostics in Ukraine, namely the microscopic agglutination test (MAT). This paper demonstrates the first results of the complex application of MAT and polymerase chain reaction (PCR) for routine verification of leptospirosis, which were first (..)

DOI: 10.33073/pjm-2020-045

Published Online: 27-December-2020

Biofilm Production Potential of Salmonella Serovars Isolated from Chickens in North West Province, South Africa

STEPHEN ABIOLA AKINOLA/ MPINDA EDOAURD TSHIMPAMBA/ MULUNDA MWANZA/ COLLINS NJIE ATEBA

Abstract Bacterial biofilms have recently gained considerable interest in the food production and medical industries due to their ability to resist destruction by disinfectants and other antimicrobials. Biofilms are extracellular polymer matrices that may enhance the survival of pathogens even when exposed to environmental stress. The effect of incubation temperatures (25°C, 37°C, and 40°C) and Salmonella serotype on biofilm-forming potentials was evaluated. Previously typed Salmonella serotypes(..)

DOI: 10.33073/pjm-2020-046

Published Online: 23-November-2020

The Effect of Lactobacillus salivarius SGL03 on Clinical and Microbiological Parameters in Periodontal Patients

MAŁGORZATA NĘDZI-GÓRA/ MARTA WRÓBLEWSKA/ RENATA GÓRSKA

Abstract The destruction of periodontal tissues during periodontitis is the result of the immune-inflammatory reactions to the bacteria of dental biofilm. Probiotics may reduce dysbiosis by the modification of the dental microbiome, which can influence the immune-inflammatory mechanisms. The aim of this study was to estimate the clinical and microbiological parameters, before and after 30 days of application of the dietary supplement containing Lactobacillus salivarius SGL03 or placebo. The stud(..)

DOI: 10.33073/pjm-2020-047

Published Online: 27-November-2020

Benefits of Soleris® over the Conventional Method for Enumeration of Microbial Load in Salacia Herbal Extract

SADANAND YEWALE/ ZEBA FARASH/ SANMAN KOLHE/ SASIDHARAN SAKKAN/ SHRINIVAS BHOPE/ PRADNYA AMBEKAR/ SRIRAM PADMANABHAN

Abstract Stems and roots of Salacia genus plants have been used as a specific remedy for early-stage diabetes, and one of the four sulphonium sulphates, salacinol is the compound responsible for the anti-diabetic activity. Salacia is prone to microbial contamination and insect infestation; hence, methods to estimate the microbial load in such plants will enhance its nutritional value. This paper highlights the novel use of Soleris® to quantify microbes of all types, namely bacteria, yeasts, mold(..)

DOI: 10.33073/pjm-2020-048

Published Online: 27-December-2020

The Incidence of Scabies and Head Lice and Their Associated Risk Factors among Displaced People in Cham Mishko Camp, Zakho City, Duhok Province, Iraq

MOHAMMAD I. ALBERFKANI/ WIJDAN M.S. MERO

Abstract This study was conducted to estimate the incidence and the effects of associated risk factors of scabies and head lice on displaced people in Cham Mishko camp, Zakho city, Duhok Province, Iraq. The study included 1300 internally displaced people (IDPs) who visited the dermatology clinic and health care center in Cham Mishko camp from January 2018 to December 2019. Participants’ bio-information was collected on special questionnaire form after receiving permission from the camp’s health (..)

DOI: 10.33073/pjm-2020-050

Published Online: 27-December-2020

Identification and Characterization of a Novel Recombinant Porcine Astrovirus from Pigs in Anhui, China

XU CHEN/ YUMIN HE/ WEINA LI/ ULLAH KALIM/ YUQING XIAO/ JIE YANG/ XIAOCHUN WANG/ SHIXING YANG/ WEN ZHANG

Abstract Porcine astroviruses (PAstVs) have wide distribution in swine herds worldwide. At present, five porcine astrovirus genotypes have been identified. In this study, using viral metagenomics, a novel PAstV strain (designated as Ahast) was identified in fecal samples from pigs in Anhui of China, and the complete genomic sequence of Ahast was obtained by assembling and PCR amplification. Genomic structural analysis indicated that Ahast had a typical ribosomal frameshifting signal, and some co(..)

DOI: 10.33073/pjm-2020-051

Published Online: 27-December-2020

Bioinformatics Analysis of Key micro-RNAs and mRNAs under the Hand, Foot, and Mouth Disease Virus Infection

SHENG LIN/ LIU YANG/ SHIBIAO WANG/ BIN WENG/ MIN LIN

Abstract To clarify crucial key micro-RNAs and mRNAs associated with hand, foot, and mouth disease (HFMD) virus infection, we conducted this bioinformatics analysis from four GEO datasets. The following datasets were used for the analysis: GSE85829, GSE94551, GSE52780, and GSE45589. Differentially expressed genes (DEGs) were acquired, and the analysis of functional and pathway enrichment and the relative regulatory network were conducted. After screening common differentially expressed miRNAs (D(..)

DOI: 10.33073/pjm-2020-052

Published Online: 27-December-2020

Multidrug-resistant Opportunistic and Pathogenic Bacteria Contaminate Algerian Banknotes Currency

LYDIA NEÏLA DJOUADI/ NADJET GUEZLANE-TEBIBEL/ KENZA MANSOURI/ HANANE BOUMERDASSI/ KARIM ARAB/ MARIE-LAURE FARDEAU/ FARIDA NATECHE

Abstract Currency is one of the most exchanged items in human communities as it is used daily in exchange for goods and services. It is handled by persons with different hygiene standards and can transit in different environments. Hence, money can constitute a reservoir for different types of human pathogens. This study aimed to evaluate the potential of Algerian banknotes to shelter opportunistic pathogenic and multiresistant bacteria. To that end, 200 circulating notes of four different denomi(..)

DOI: 10.33073/pjm-2020-053

Published Online: 27-December-2020

A Novel Improved Gram Staining Method Based on the Capillary Tube

KONG-YANG WU/ TONG-XIANG YANG

Abstract In this work, an exploratory study was conducted to examine Gram staining based on the capillary tube. Each Gram staining step for all bacterial strains tested was completed in capillary tubes. The results showed that different Gram staining morphologies were clearly visible in the capillary tubes. The results presented here demonstrated that the improved method could effectively distinguish between Gram-positive and Gram-negative bacteria, and only small volumes of reagents were requir(..)

DOI: 10.33073/pjm-2020-043

Published Online: 23-November-2020

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