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Immunohematology

Journal of Blood Group Serology and Molecular Genetics

American National Red Cross

Subject: Medical Laboratory Technology

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ISSN: 0894-203X
eISSN: 1930-3955

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VOLUME 32 , ISSUE 4 (December 2016) - List of articles

Distribution of blood groups in the Iranian general population

Ehsan Shahverdi/ Mostafa Moghaddam/ Ali Talebian/ Hassan Abolghasemi

We report the first study of antigen and phenotype prevalence within various blood group systems in the Iranian general population. In this retrospective study, samples from 3475 individuals referred to the Immunohematology Reference Laboratory of the Iranian Blood Transfusion Organization, Tehran, Iran, for paternity testing from 1998 to 2008 were additionally tested for red blood cell (RBC) antigens in the Rh, Kell, Kidd, Duffy, MNS, Lutheran, P1PK, and Xg blood group systems. The antigen test(..)

DOI: 10.21307/immunohematology-2019-058

Acute hemolytic transfusion reaction attributed to anti-Ata

Jay S. Raval/ Sarah K. Harm/ Bethann Wagner/ Darrell J. Triulzi/ Mark H. Yazer

Anti-Ata is a rare alloantibody that can be clinically significant. We report a case of a woman who, after emergency-released uncrossmatched red blood cell transfusion, experienced an acute hemolytic transfusion reaction attributed to anti-Ata. The case presented herein highlights the importance of recognizing that anti-Ata may indeed cause acute hemolytic reactions.

DOI: 10.21307/immunohematology-2019-059

Human platelet antigen allelic diversity in Peninsular Malaysia

Wan Ubaidillah Wan Syafawati/ Zulkafli Zefarina/ Zafarina Zafarina/ Mohd Nazri Hassan/ Mohd Nor Norazmi/ Sundararajulu Panneerchelvam/ Geoffrey Keith Chambers/ Hisham Atan Edinur

Human platelet antigens (HPAs) are polymorphic and immunogenic glycoproteins encoded by biallelic genes on human chromosome 17 (HPA-1 to -4 and HPA-6 to -11), chromosome 5 (HPA-5), and chromosome 6 (HPA-15) and expressed on the surface of platelets. In the present study, we typed seven HPA loci (HPA-1 to -6 and HPA-15) by polymerase chain reaction using sequence-specific primer and sequence-based typing in 166 blood samples representing three Orang Asli groups (Semang, Senoi, and Proto-Malays) t(..)

DOI: 10.21307/immunohematology-2019-060

A detailed flow cytometric method for detection of low-level in vivo red blood  cell–bound IgG, IgA, and IgM

Wendy Beres/ Geralyn M. Meny/ Sandra Nance

Flow cytometric methods are commonly used to analyze white blood cell surface antigen expression. We developed a flow cytometric method to detect red blood cell (RBC)-bound immunoglobulin (Ig)G, IgA, and IgM. RBCs were washed; incubated with fluorescein isothiocyanate (FITC)-conjugated anti-IgG, -IgA, or -IgM; washed; and analyzed on the flow cytometer. The method was optimized by determining the dilution of FITC-conjugated anti-IgG, -IgA, and -IgM providing the greatest amount of fluorescence w(..)

DOI: 10.21307/immunohematology-2019-061

Trends of ABO and Rh phenotypes in transfusion-dependent patients in Pakistan

Nida Anwar/ Munira Borhany/ Saqib Ansari/ Sana Khurram/ Uzma Zaidi/ Imran Naseer/ Muhammad Nadeem/ Tahir Shamsi

The objective of this study was to determine the prevalence of ABO and Rh phenotypes in the general Pakistan population. This information could be used to help reduce the rate of alloimmunization in patients with blood disorders, such as thalassemia major, who require frequent blood transfusions. A total of 242 patients with blood disorders requiring frequent blood transfusions were enrolled in the study. ABO and Rh typing was performed on samples from these patients using tube and gel methods. (..)

DOI: 10.21307/immunohematology-2019-062

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