Control of the tomato leaf miner, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) larvae in laboratory using entomopathogenic nematodes from subtropical environment

Bonginkhosi E. Dlamini/ Nelisiwe Dlamini/ Michael T. Masarirambi/ Nxumalo Kwanele A.

Abstract Tomato (Solanum esculentum) is one of the vegetable crops grown by both smallholder and commercial farmers in the Kingdom of Eswatini. Tomato leaf miner, Tuta absoluta M. is a major insect pest of tomatoes resulting in reduced tomato yields throughout the country. The study investigated the virulence of two sub-tropical EPN species on T. absoluta larvae. Steinernema yirgalemense and S. jeffreyense at different concentrations (0, 20, 40, 60 IJs/insect) were screened for efficacy (i.e. mo(..)

DOI: 10.21307/jofnem-2020-013

First report of Meloidogyne enterolobii infecting Japanese blue berry tree (Elaeocarpus decipiens) in Florida, USA

M. R. Moore/ J. A. Brito/ S. Qiu/ C. G. Roberts/ L. A. Combee

Abstract In October 2019, samples of galled roots with rhizosphere soil were collected from declining Elaeocarpus decipiens in Hernando County, Florida. Extracted root-knot nematodes were identified by both molecular and morphological methods as Meloidogyne enterolobii. This is a first report of this regulated root-knot nematode on Elaeocarpus decipiens in Florida.

DOI: 10.21307/jofnem-2020-005

The effects of Meloidogyne incognita and Heterodera glycines on the yield and quality of edamame (Glycine max l.) in Arkansas

J. E. Wilkes/ T. L. Kirkpatrick

Abstract In 2012, the first domestic commercial edamame processing plant was established in Arkansas and edamame production was contracted out to local growers. Although the state is a major soybean producer, studies of nematode effects on edamame are limited. A survey of nematode genera and density in 64 contracted edamame production fields was conducted in 2013 and 2014. In both years, Meloidogyne and Heterodera were present in less than half of the surveyed fields while Pratylenchus was the m(..)

DOI: 10.21307/jofnem-2020-012

Fungal communities associated with Heterodera glycines and their potential in biological control: a current update

Deepak Haarith/ Kathryn E. Bushley/ Senyu Chen

Abstract The soybean cyst nematode (SCN) is the most important pest on soybean, a major crop worldwide. The SCN is considered both parasitic and pathogenic as it derives nutrition from the host and manipulates host physiology to do so. Currently, there are no commercially available chemicals that are specific, environmentally safe and cost effective to control SCN levels. Crop rotation, use of host resistance and other cultural practices remain the main management strategies. The need for biopro(..)

DOI: 10.21307/jofnem-2020-022

Genome sequence of the root-knot nematode Meloidogyne luci

Nik Susič/ Georgios D. Koutsovoulos/ Cristian Riccio/ Etienne G. J. Danchin/ Mark L. Blaxter/ David H. Lunt/ Polona Strajnar/ Saša Širca/ Gregor Urek/ Barbara Gerič Stare

Abstract Root-knot nematodes from the genus Meloidogyne are polyphagous plant endoparasites and agricultural pests of global importance. Here, we report the high-quality genome sequence of Meloidogyne luci population SI-Smartno V13. The resulting genome assembly of M. luci SI-Smartno V13 consists of 327 contigs, with an N50 contig length of 1,711,905 bp and a total assembly length of 209.16 Mb.

DOI: 10.21307/jofnem-2020-025

A draft genome of a field-collected Steinernema feltiae strain NW

Zhen Fu/ Yuxiang Li/ Axel A. Elling/ William E. Snyder

Abstract Advances in sequencing technologies have accelerated our understanding of the complex genetic network of organisms and genomic divergences that are linked to evolutionary processes. While many model organisms and laboratory strains have been sequenced, wild populations are underrepresented in the growing list of sequenced genomes. Here, we present a de novo assembly of Steinernema feltiae, strain NW, collected from a working agricultural field in south central Washington, USA. Leveragin(..)

DOI: 10.21307/jofnem-2020-003

Molecular approach to confirm traditional identification of Radopholus similis sampled in Tanzania

Doreen M. Mgonja/ Gladness E. Temu/ Joseph C. Ndunguru/ Magreth F. Mziray/ Sylvester L. Lyantagaye/ Nessie D. Luambano

Abstract Banana (Musa spp. L.) is an important staple food and cash crop for about 30% of the population in Tanzania; however, the burrowing plant-parasitic nematode Radopholus similis causes black head disease and toppling in banana plants, which results in yield losses. We collected and identified 80 specimens of R. similis from four agro-ecological zones in Tanzania using morphological characters. We then used universal and specific R. similis primers to amplify the small subunit, internal tr(..)

DOI: 10.21307/jofnem-2020-020

Silver nanoparticles as a potential nematicide against Meloidogyne graminicola

Richa Baronia/ Puneet Kumar/ S. P. Singh/ R. K. Walia

Abstract Plant-parasitic nematodes cause severe damage to the various agricultural crops, leading to economic losses for farmers. Therefore, identification and development of novel and environmentally benign nematicides is critically important. In this study, a silver nanoparticle (AgNP) formulation was synthesized, characterized, and investigated as a potential nematicide against rice root-knot nematode, Meloidogyne gramnicola, on rice (Oryza sativa). A series of lab assays (water and sand scre(..)

DOI: 10.21307/jofnem-2020-002

Description of Laimaphelenchus sinensis n. sp. (Nematoda: Aphelenchoididae) from declining Chinese pine, Pinus tabuliformis in Beijing, China

Jianfeng Gu/ Munawar Maria/ Yiwu Fang/ Lele Liu/ Yong Bian/ Xianfeng Chen

Abstract Laimaphelenchus sinensis n. sp. isolated from declining Chinese pine, Pinus tabuliformis Carrière, is described and characterized morphologically and molecularly. The new species has four incisures in the lateral field and the excretory pore situated posterior to the nerve ring; the female has a vulval flap and vaginal sclerotization is quite prominent in majority of specimens. The female tail is conoid, ventrally curved having a single stalk-like terminus with 8 to 10 projections. The (..)

DOI: 10.21307/jofnem-2020-019

Molecular and morphological characterization of the alfalfa cyst nematode, Heterodera medicaginis, from Utah

Zafar A. Handoo/ Andrea M. Skantar/ Saad L. Hafez/ Mihail R. Kantor/ Maria N. Hult/ Stephen A. Rogers

Abstract In the spring of 2019, a cyst nematode was discovered from soil samples collected from an alfalfa field in Millard County, Utah. The soil samples were submitted to one of us (SH), who extracted the nematode cysts and sent them to the USDA-ARS, Mycology and Nematology Genetic Diversity and Biology Laboratory (MNGDBL), Beltsville, MD for morphological and molecular identification. Cysts and living nematode juveniles (J2) recovered from the cysts were examined morphologically and molecular(..)

DOI: 10.21307/jofnem-2020-015

Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

L.K. Carta/ S. Li

Abstract Generating DNA markers for microscopic plant parasitic nematodes can be especially difficult if only a few valuable, tiny specimens are available. Providing a reliable maximum amount of unambiguous genetic information from single nematodes is especially important when identifying damaging, regulated nematodes of importance to trade where a few nucleotide differences in diagnostic markers are significant. There are many possible reasons for difficulty amplifying unpurified nematode DNA f(..)

DOI: 10.21307/jofnem-2020-016

Essential oils for managing Pratylenchus penetrans on Easter lilies

B. B. Westerdahl/ D. Giraud/ L. J. Riddle/ C. A. Anderson

Abstract Easter lily bulbs for greenhouse forcing are produced in Del Norte County, California and Curry County, Oregon, USA. Pratylenchus penetrans infestation seriously affects growth of field grown bulbs. During two consecutive years of field trials containing 22 treatments, commercially prepared formulations of essential oils (EOs) were compared to an untreated control and to a standard chemical fumigant treatment (FU) (1,3-dichloropropene and metam sodium) applied preplant followed by phora(..)

DOI: 10.21307/jofnem-2020-010

Characterization of root-knot nematodes infecting mulberry in Southern China

Pan Zhang/ Hudie Shao/ Chunping You/ Yan Feng/ Zhenwen Xie

Abstract China is one of the largest producers of mulberry in the world. With the development of the sericulture industry, several pests and diseases have occurred in rapid succession, chief among which is the root-knot nematode disease affecting mulberry. According to the China cocoon and silk exchange, cocoon prices have doubled since the beginning of 2009 and rose to 92,700 yuan ($135,770) per tonne in mid-April 2010. According to customs statistics, in the first eight months of 2011, China’s(..)

DOI: 10.21307/jofnem-2020-004

First report of a stunt nematode Tylenchorhynchus zeae on corn in Gansu Province, China

Zhi Peng Xu/ Hui Xia Li/ Yong Gang Liu/ Bao Cang Ren/ Chun Hui Ni/ Jin Hui Ma

Abstract During a survey of plant parasitic nematodes in 2019, at Gansu Province, China, the stunt nematode Tylenchorhynchus zeae was found parasitizing corn seedlings. Females, males and juveniles of T. zeae were observed on soil and roots samples of corn after processing and extraction. This population of stunt nematodes was identified based on morphological and by sequencing the ITS1 region of rDNA and D2/D3 fragments of the 28 S rRNA. The ITS1 and the D2/D3 sequences of this population, shar(..)

DOI: 10.21307/jofnem-2020-009

First report of root-knot nematode, Meloidogyne arenaria, on lavender in Turkey

Tevfik Özalp/ Gonca Könül/ Önder Ayyıldız/ Adnan Tülek/ Zübeyir Devran

Abstract Lavender is a medicinal and aromatic plant that is widely grown in Turkey. Gall symptoms were observed on roots of lavender (Lavandula angustifolia Mill.) collected from Kırklareli and Edirne provinces. Egg masses were collected from galled roots. DNA isolated from all samples was screened by species-specific primers belonging to the most common species of root-knot nematodes and M. arenaria was the only species that was identified in all of the samples analyzed. This is the first repor(..)

DOI: 10.21307/jofnem-2020-008

Description of Seinura italiensis n. sp. (Tylenchomorpha: Aphelenchoididae) found in the medium soil imported from Italy

Jianfeng Gu/ Munawar Maria/ Lele Liu/ Majid Pedram

Abstract Seinura italiensis n. sp. isolated from the medium soil imported from Italy is described and illustrated using morphological and molecular data. The new species is characterized by having short body (477 (407-565) µm and 522 (469-590) µm for males and females, respectively), three lateral lines, stylet lacking swellings at the base, and excretory pore at the base or slightly anterior to base of metacorpus; females have 58.8 (51.1-69.3) µm long post-uterine sac (PUS), elongate conical ta(..)

DOI: 10.21307/jofnem-2020-018

Soil texture, infective juvenile concentration, and soil organic matter influence the efficacy of Steinernema feltiae isolate Lican Ray

Gabriela Lankin/ Giselle Vidal-Retes/ Geraldine Allende/ Carlos Castaneda-Alvarez/ Ernesto San-Blas/ Erwin Aballay

Abstract The influence of infective juveniles (IJs) concentration, soil texture, IJ-host distance and organic matter (OM) content, at different decomposition degree, on the activity of the nematode Steinernema feltiae isolate Lican Ray (LR) was examined using Galleria mellonella larvae. Bioassays were conducted in tubes of varied length, filled with soil of different textures, placed either vertically or horizontally. In the concentration assay, highest IJ concentrations caused maximum larval mo(..)

DOI: 10.21307/jofnem-2020-007

A new rare nematode Nothocriconemoides hangzhouensis n. sp. (Nematoda: Criconematidae) from Hangzhou, China

Munawar Maria/ Wentao Miao/ Ruihang Cai/ Pablo Castillo/ Jingwu Zheng

Abstract The Family Criconematidae is commonly referred as ring nematodes that include some members with economic importance as plant parasites. During a recent nematode inventory survey at Zhejiang Province, China, a new species of genus Nothocriconemoides was detected in the rhizosphere of elm tree. Nothocriconemoides hangzhouensis n. sp. can be characterized by the female body having annuli with fine longitudinal striations and 2 to 3 anastomoses at the posterior half of the body. The first c(..)

DOI: 10.21307/jofnem-2020-006

Transcriptomic analysis of Bursaphelenchus xylophilus treated by a potential phytonematicide, punicalagin

Qun-Qun Guo/ Gui-Cai Du/ Ting-Ting Zhang/ Mei-Juan Wang/ Chao Wang/ Hong-Tao Qi/ Rong-Gui Li

Abstract Punicalagin showed significant nematotoxic activity against pine wood nematode (PWN), Bursaphelenchus xylophilus, in the authors’ previous research. The authors performed high-throughput transcriptomic sequencing of punicalagin-treated nematodes to generate clues for its nematotoxic mechanism of action. The authors identified 2,575 differentially expressed genes, 1,428 of which were up-regulated and 1,147 down-regulated. Based on a comprehensive functional in silico analysis, the author(..)

DOI: 10.21307/jofnem-2020-001

Natural occurrence and distribution of entomopathogenic nematodes (Steinernematidae, Heterorhabditidae) in Viti Levu, Fiji Islands

Sumeet Kour/ Uma Khurma/ Gilianne Brodie/ Selcuk Hazir

Abstract In Fiji, little or no attention has been given to entomopathogenic nematodes (EPNs) in biocontrol programs due to the lack of awareness about their occurrence and distribution in Fiji. A survey of EPNs was conducted for the first time in Fiji Islands in 2012 and 2013, throughout the eight provinces in Viti Levu to determine the occurrence and distribution across different habitat in Viti Levu. The soil samples from various habitats were collected and assayed for the presence of EPN usin(..)

DOI: 10.21307/jofnem-2020-017

Effect of an Alltech soil health product on entomopathogenic nematodes, root-knot nematodes and on the growth of tomato plants in the greenhouse

Anusha Pulavarty/ Karina Horgan/ Thomais Kakouli-Duarte

Abstract An organic product that consists of proprietary blend of fermentation and plant extracts with micronutrients (ACS 5075, Alltech®, Inc., Nicholasville, KY USA) was evaluated against four strains of entomopathogenic nematodes (EPN): Steinernema feltiae (SB12(1), a wild enviroCORE strain and a commercial form e-NEMA), Steinernema carpocapsae (e-NEMA), and Heterorhabditis bacteriophora. The effects on egg hatching and survival of root-knot nematodes (RKN) were also examined. The sensitivity(..)

DOI: 10.21307/jofnem-2020-014

Elimination of Tobacco rattle virus from viruliferous Paratrichodorus allius in greenhouse pot experiments through cultivation of castle russet

Richard A. Quick/ Launa Cimrhakl/ Hassan Mojtahedi/ Vidyasagar Sathuvalli/ Maximilian J. Feldman/ Charles R. Brown

Abstract Corky ringspot (CRS) is a widespread potato tuber necrotic disease caused by Tobacco rattle virus (TRV) infection. In the Pacific Northwest, this virus is transmitted by the stubby root nematode (SRN) within the genus Paratrichodorus. Remediating CRS affected fields is a major challenge that can be mitigated by growing plant varieties that are resistant to TRV infection. Growing alfalfa has been shown to reduce TRV levels in CRS infested fields over time but the development of a potato (..)

DOI: 10.21307/jofnem-2020-011

Characterization of Vittatidera zeaphila (Nematoda: Heteroderidae) from Indiana with molecular phylogenetic analysis of the genus

Andrea M. Skantar/ Zafar A. Handoo/ Mihail R. Kantor/ Lynn K. Carta/ Jamal Faghihi/ Virginia Ferris

Abstract In the summer of 2016, a field of corn (Zea mays) in Spencer County, Indiana was observed with heavily stunted plants, and from the affected roots a large number of cysts were recovered. Soil samples were submitted to one of us (JF), who extracted the nematode cysts and sent them to the USDA-ARS, Mycology and Nematology Genetic Diversity and Biology Laboratory (MNGDBL), Beltsville, MD for morphological and molecular identification. Cysts and the recovered second-stage juveniles (J2) tha(..)

DOI: 10.21307/jofnem-2020-024

First report of the sting nematode Belonolaimus longicaudatus infecting bermudagrass in Barbados

P. Mc Groary/ W. Ye/ E. Nangle

Abstract In 2016, “Tifdwarf” hybrid bermudagrass (Cynodon dactylon (L) Pers. × C. transvaalensis Burtt-Davy) grown on a golf green built to the United States Golf Association recommendations in Barbados started to show irregular significant chlorotic patches followed by gradual thinning and decline of turfgrass. A survey was conducted in May 2016 to determine the presence of plant-parasitic nematodes. The results revealed the presence of the plant-parasitic sting nematode Belonolaimus longicauda(..)

DOI: 10.21307/jofnem-2020-021

First report of Bursaphelenchus fungivorus (Nematoda: Aphelenchida) in Italy and an overview of nematodes associated with Crocus sativus L.

Giulia Torrini/ Agostino Strangi/ Stefania Simoncini/ Maria Luppino/ Pio Federico Roversi/ Leonardo Marianelli

Abstract Saffron is one of the most precious spices in the world. It is derived from the dried stigmas of the Crocus sativus L. flowers. This plant is triploid sterile and propagated by corms. As a subterranean organ, the corm can come into contact with different nematodes. In this contribution Bursaphelenchus fungivorus was reported for the first time in Italy. It was found associated with C. sativus corms and characterized based on morphological and morphometrical characteristics for this spec(..)

DOI: 10.21307/jofnem-2020-023

Examine medicinal plants from South Africa for suppression of Meloidogyne incognita under glasshouse conditions

Mbokota Candy Khosa/ Zakheleni Dube/ Dirk De Waele/ Mieke Stefanie Daneel

Abstract The nematicidal activity of crudely milled powders of stems, leaves, and bulbs of Cassia abbreviata, Cissus cactiformis, Euphorbia ingens, Ipomoea kituiensis, Synadenium cupulare, Senna petersiana, Urigenia sanguinea, Maerua angolensis, and Tabernaemontana elegans on eggs and J2 population densities of Meloidogyne incognita race 2 on tomato was examined under glasshouse conditions. These plant species have medicinal properties and are being used in South Africa by traditional healers as(..)

DOI: 10.21307/jofnem-2020-029

First report of the root-knot nematode, Meloidogyne morocciensis infecting peach in Southern Brazil

W. R. Silva/ C. P. Machaca-Calsin/ C. B. Gomes

Abstract The peach tree (Prunus persica) is the third most cultivated temperate fruit species in Brazil. In August 2017, peach seedling plants showing symptoms of stunting and multiple galls in the roots were detected in the municipality of Pelotas, state of Rio Grande do Sul, Brazil. Samples of peach roots were collected and they were subsequently processed to obtain eggs and second-stage juveniles (J2), to determine the number of nematodes per gram of roots and to proceed with morphological st(..)

DOI: 10.21307/jofnem-2020-032

Root-knot nematodes demonstrate temporal variation in host penetration

Shova Mishra/ Peter DiGennaro

Abstract Root-knot nematodes (RKN; Meloidogyne spp.) are obligate plant parasites that require constant communication with their host to establish and maintain specialized feeding cells. The intimacy of this interaction likely requires constant monitoring of host biology and behavior. As plant processes follow tightly regulated circadian and diurnal patterns, RKN may use similar cues to regulate aspects of this symbiosis. We interrogated RKN biology within the context of host diurnal rhythms thr(..)

DOI: 10.21307/jofnem-2020-037

First molecular characterization of an Iranian population of Schistonchus caprifici (Gasparrini, 1864) Cobb, 1927 (Rhabditida; Aphelenchoidea)

Hadi Karimipour Fard/ Hamid Zare

Abstract A population of Schistonchus caprifici was recovered in Iran during the present study. The molecular characters of this population were studied using partial sequences of large subunit ribosomal DNA (LSU rDNA D2-D3) for the first time, and its phylogenetic relationships with other isolates of the species were discussed. Compared to previous available data, no remarkable genetic distance was observed between the presently studied isolate and other isolates.

DOI: 10.21307/jofnem-2020-039

Nematicide efficacy at managing Meloidogyne arenaria and non-target effects on free-living nematodes in peanut production

Zane J. Grabau/ Mark D. Mauldin/ Alemayehu Habteweld/ Ethan T. Carter

Abstract Meloidogyne arenaria (peanut root-knot nematode (PRKN)) is a major pest of peanut. Nematicide application is an important tool for the management of PRKN. Nematicides with minimal effects on free-living nematodes are desired. Fluopyram nematicide is recently introduced in peanut production and needs to be assessed. The objective of this research is to evaluate fluopyram and the established nematicides 1,3-Dichloropropene (1,3-D) and aldicarb for efficacy at managing PRKN and impacts on (..)

DOI: 10.21307/jofnem-2020-028

Nematicide influence on cotton yield and plant-parasitic nematodes in conventional and sod-based crop rotation

Lesley A. Schumacher/ Zane J. Grabau/ David L. Wright/ Ian M. Small/ Hui-Ling Liao

Abstract Plant-parasitic nematodes (Rotylenchulus reniformis (reniform, RN), Helicotylenchus dihystera (spiral), and Mesocriconema ornatum (ring)) and yield were investigated in cotton phases of conventional (peanut–cotton–cotton) and sod-based (bahiagrass–bahiagrass–peanut–cotton) rotations with or without irrigation and fluopyram nematicide at a long-term research site, established in 2000, in Quincy, Florida, USA. Objectives were to determine impacts of nematicide application on cotton yield (..)

DOI: 10.21307/jofnem-2020-034

De novo transcriptome sequencing and analysis of Anisakis pegreffii (Nematoda: Anisakidae) third-stage and fourth stage larvae

U-Hwa Nam/ Jong-Oh Kim/ Jeong-Ho Kim

Abstract Anisakis pegreffii is known as one of the causes of a fish-borne zoonosis, anisakidosis. Despite its significant public health and food hygiene impacts, little is known of the pathogenesis, genetic background of this parasite, at least partly due to the lack of genome and transcriptome information. In this study, RNA-seq and de novo assembly were conducted to obtain transcriptome profiles of the A. pegreffii third and fourth larvae. The third stage larvae (APL3) were collected from chub(..)

DOI: 10.21307/jofnem-2020-041

A new stunt nematode, Geocenamus chengi n. sp. (Nematoda: Merliniinae) in the rhizosphere of tea (Camellia sinensis) from Zhejiang Province, China

Munawar Maria/ Wentao Miao/ Pablo Castillo/ Jingwu Zheng

Abstract The tea plant is native to China; the country has the greatest tea production areas in the world. In an attempt to investigate the nematode biodiversity associated with the tea plantations of Hangzhou, Zhejiang Province, a population of stunt nematode was detected. This group of nematodes is comprised of migratory ecto-parasites of roots and can subsist on a variety of host plants. Therefore, the detected population was studied carefully using the integrative taxonomy approach and ident(..)

DOI: 10.21307/jofnem-2020-042

Spatial distribution and risk area assessment of Aphelenchoides besseyi using geostatistical approaches in Giridih district of Jharkhand, India

Sandip Mondal/ Matiyar Rahaman Khan/ Abhishek Mukherjee

Abstract Distributed widely across the rice growing regions of India, the rice leaf and bud nematode (Aphelenchoides besseyi Christie 1942) can cause substantial yield loss in rice. Whitening of leaf tips is the characteristics damage symptom of this nematode and therefore it is popularly referred to as the rice white tip nematode (RWTN). While information on the damage severity of RWTN is available from others parts of the country, to date, no information is available from the state of Jharkhan(..)

DOI: 10.21307/jofnem-2020-033

Plant-parasitic nematodes associated with the root zone of hop cultivars planted in a Florida field soil

Tristan T. Watson/ Marco Suarez/ Zhanao Deng/ Johan A. Desaeger

Abstract In early 2016, hop plants were introduced into Florida. By late 2016, the hop plants were showing stunted growth and were heavily parasitized by Meloidogyne javanica. In this study, we determined host susceptibility of 14 hop cultivars to M. javanica in a greenhouse experiment and monitored population development of plant-parasitic nematode species in the root zone of 17 hop cultivars planted in three newly established hop yards in Florida. Plant-parasitic nematodes in the rooting zone (..)

DOI: 10.21307/jofnem-2020-040

Genetic intraspecific diversity of Meloidogyne javanica parasitizing vegetables in southern Iran

Reza Ghaderi/ Ali Asghar Dehghan/ Abbas Mokaram Hesar/ Akbar Karegar

Abstract In order to investigate different species of root-knot nematodes associated with vegetable production in southern regions of Iran, 37 populations of the most predominant species, Meloidogyne javanica, were recovered. Morphological and morphometric studies were carried on the characters of females, males, J2s and perineal patterns. Species-specific Sequence Characterized Amplified Region (SCAR) primers confirmed morphological studies, and all these populations produced specific band in 6(..)

DOI: 10.21307/jofnem-2020-035

First report of molecular characterization and phylogeny of Trichuris fossor Hall, 1916 (Nematoda: Trichuridae)

Malorri R. Hughes/ Deborah A. Duffield/ Dana K. Howe/ Dee R. Denver

Abstract Because species of Trichuris are morphologically similar and ranges of host preference are variable, using molecular data to evaluate species delineations is essential for properly quantifying biodiversity of and relationships within Trichuridae. Trichuris fossor has been reported from Thomomys spp. (Rodentia: Geomyidae, ‘pocket gophers’) hosts based on morphological features alone. Partial 18S rRNA sequences for specimens identified as T. fossor based on morphology, along with sequence(..)

DOI: 10.21307/jofnem-2020-036

Differences in parasitism of root-knot nematodes (Meloidogyne spp.) on oilseed radish and oat

Negin Hamidi/ Abolfazl Hajihassani

Abstract Oilseed radish and oat are cool season annual crops that are potentially used as “trap” or “biofumigant” crops for the suppression of plant-parasitic nematodes in soil. Cultivars of oilseed radish (Carwoodi, Cardinal, Final, Image, Concorde, Control, Eco-Till, Karakter and Cannavaro), white (Tachiibuki) and black (Pratex) oats were evaluated for their ability to reduce reproduction of three root-knot nematode species: Meloidogyne javanica, M. incognita race 3, and M. arenaria race 1. Ne(..)

DOI: 10.21307/jofnem-2020-043

Cephalenchus driekieae n. sp. (Nematoda: Tylenchidae) from South Africa, a new member of the genus with a long pharyngeal overlap

Milad Rashidifard/ Gerhard Du Preez/ Joaquín Abolafia/ Majid Pedram

Abstract Cephalenchus driekieae n. sp. is described and illustrated based on its morphological, morphometric, and molecular characteristics. This new species is mainly characterized by its short stylet 11.5 to 13.0 µm, and 13.5 to 17.5 µm long pharyngeal overlap extending over the intestine. It could further be delimited by 451 to 526 µm long females with a prominently annulated cuticle, dorso-ventral amphidial openings as shown using scanning electron microscopy (SEM), four lines in the lateral(..)

DOI: 10.21307/jofnem-2020-031

First report of southern root-knot nematode, Meloidogyne incognita, infecting pomegranate, Punica granatum, in Peru

Ricardo Andreé Vega-Callo/ María Yaquelin Mendoza-Lima/ Nataly Ruth Mamani-Mendoza/ Leslie Sharon Lozada-Villanueva/ Juan José Tamo-Zegarra/ Teodocia Gloria Casa-Ruiz/ Cristiano Bellé

Abstract Punica granatum plants showing symptoms caused by root-knot nematodes were detected in the municipality of Majes, Arequipa, Peru. Based on the morphological, esterase phenotypes, and molecular analyses of the mitochondrial DNA region between the cytochome oxidase subunit II and 16 S rRNA genes (mtDNA) and species-specific sequence characterized amplified region, the causal agent of the observed symptoms was identified as Meloidogyne incognita. Pathogenicity was confirmed by fulfilling a(..)

DOI: 10.21307/jofnem-2020-026

Mitochondrial COI gene is valid to delimitate Tylenchidae (Nematoda: Tylenchomorpha) species

Mengxin Bai/ Xue Qing/ Kaikai Qiao/ Xulan Ning/ Shun Xiao/ Xi Cheng/ Guokun Liu

Abstract Tylenchidae is a widely distributed soil-inhabiting nematode family. Regardless their abundance, molecular phylogeny based on rRNA genes is problematic, and the delimitation of taxa in this group remains poorly documented and highly uncertain. Mitochondrial Cytochrome Oxidase I (COI) gene is an important barcoding gene that has been widely used species identifications and phylogenetic analyses. However, currently COI data are only available for one species in Tylenchidae. In present stu(..)

DOI: 10.21307/jofnem-2020-038

Morphological and molecular characterization of Acrobeloides saeedi Siddiqi, De Ley and Khan, 1992 (Rhabditida, Cephalobidae) from India and comments on its status

Aasha Rana/ Aashaq Hussain Bhat/ Suman Bhargava/ Ashok Kumar Chaubey/ Joaquín Abolafia

Abstract Two cultured populations of Acrobeloides saeedi are described from India. Morphologically and morphometrically this material agrees with other species of the Maximus-group (A. bodenheimeri, A. longiuterus, and A. maximus), especially with A. longiuterus. However, molecular studies based on 18 S, 28 S and ITS rDNA confirmed the Indian material is well differentiated from all of these species. According to this, A. saeedi is considered a valid taxon distinguished mainly from A. bodenheime(..)

DOI: 10.21307/jofnem-2020-027

Morphological and molecular characterization of Pungentus sufiyanensis n. sp. and additional data on P. engadinensis (Altherr, 1950) Altherr, 1952 (Dorylaimida: Nordiidae) from northwest of Iran

Nasir Vazifeh/ Gholamreza Niknam/ Habibeh Jabbari/ Reyes Peña-Santiago

Abstract Two species of the genus Pungentus, one new and one known, collected in natural vegetation and cultivated soils in northwest of Iran, are studied. Pungentus sufiyanensis n. sp. is characterized by its 1.22 to 1.57 mm long body, offset lip region by a constriction and 7 to 9 μm broad, 18 to 21 μm long odontostyle, 304 to 348 μm long neck, 133 to 161 μm long esophageal expansion, mono-opisthodelphic female genital system without anterior uterine sac, slightly backward directed vagina, abs(..)

DOI: 10.21307/jofnem-2020-030

Obituary

Manzoor H. Soomro

DOI: 10.21307/jofnem-2020-044

Panagrolaimus superbus tolerates hypoxia within Gallium metal cage: implications for the understanding of the phenomenon of anhydrobiosis

Danyel Fernandes Contiliani/ Yasmin de Araújo Ribeiro/ Vitor Nolasco de Moraes/ Tiago Campos Pereira

Abstract Panagrolaimus superbus nematodes are able to tolerate desiccation by entering into a peculiar state of suspended animation known as anhydrobiosis. When desiccated, anhydrobiotic organisms are also able to tolerate other physical stresses, as high and low levels of temperature and pressure. Here, we decided to investigate the tolerance of desiccated P. superbus to an unprecedented double stress – hypoxia within 99.99% Gallium (Ga) metal cage. The authors observed that regardless of the e(..)

DOI: 10.21307/jofnem-2020-046

First report of Ovomermis sinensis (Nematoda: Mermithidae) parasitizing fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae) in China

Bingjiao Sun/ Fen Li/ Xiaorui He/ Fengqin Cao/ Elizabeth Bandason/ David Shapiro-Ilan/ Weibin Ruan/ Shaoying Wu

Abstract Spodoptera frugiperda invaded China in the end of 2018 and has caused severe damage to maize and other crops. Several S. frugiperda naturally parasitized by nematodes were observed in Hainan Province, China. The morphological characteristics based on the results of scanning electron microscopy indicated that the nematode belongs to the family Mermithidae. Additionally, coding sequences for the 18 S and 28 S rDNA were amplified from the nematode genome, and phylogenetic analysis revealed(..)

DOI: 10.21307/jofnem-2020-050

Prevalence of the root lesion nematode virus (RLNV1) in populations of Pratylenchus penetrans from North America

Paulo Vieira/ Amy Peetz/ Benjamin Mimee/ Kanan Saikai/ Dimitre Mollov/ Ann MacGuidwin/ Inga Zasada/ Lev G. Nemchinov

Abstract Root lesion nematode virus 1 (RLNV1) was discovered in the migratory endoparasitic nematode species Pratylenchus penetrans. It was found in a P. penetrans population collected from soil samples in Beltsville, Maryland, USA. In this study, the distribution of the RLNV1 in 31 geographically distinct P. penetrans populations obtained from different crops was examined. The results demonstrate that RLNV1 is widespread in North American populations of P. penetrans and exhibits low genetic var(..)

DOI: 10.21307/jofnem-2020-045

Amphimermis enzoni n. sp. (Nematoda: Mermithidae) parasitizing damselflies and dragonflies in Argentina

José Matias Rusconi/ Cristian Di Battista/ Darío Balcazar/ Matías Rosales/ María Fernanda Achinelly

Abstract A mermithid nematode was found parasitizing nymphs of dragonflies and damselflies. The host specimens were collected from the stream Cajaravilla, Magdalena, Buenos Aires state, Argentina. In this work, we described Amphimermis enzoni n. sp., a nematode new to science. Nematodes were identified through morphological and molecular methods. The combination of the following characters separates A. enzoni n. sp. from other members of the genus Amphimermis Steiner: long and S-shaped vagina, t(..)

DOI: 10.21307/jofnem-2020-051

First report of Rotylenchulus reniformis infecting turmeric in Vietnam and consequent damage

Huu Tien Nguyen/ Quang Phap Trinh/ Thi Duyen Nguyen/ Wim Bert

Abstract Turmeric (Curcuma longa L.) is one of the common medicinal crops of high economical value in Vietnam. A survey in the Central Highlands of Vietnam revealed a turmeric growing area showing serious disease symptoms, including stunting of the plant, yellowing or darkening of the leaf margins and tips, and underdeveloped dry and rotten rhizomes. An inspection for plant pathogens in soil samples from this area revealed a high density and frequency of Rotylenchus reniformis, with a significan(..)

DOI: 10.21307/jofnem-2020-053

First report of root-knot nematodes (Meloidogyne species) infecting Chinese Elm (Ulmus parvifolia) in Florida, USA

M. R. Moore/ J. A. Brito/ S. Qiu/ C. G. Roberts/ L. A. Combee

Abstract Samples of galled roots, resembling those induced by root-knot nematodes, and rhizosphere soil were collected from potted plants of Ulmus parvifolia cvs. Allee and Drake in Lake County, Florida. Nematode species were identified using both molecular analysis and morphology of perineal patterns. Meloidogyne enterolobii and M. javanica were identified from U. parvifolia cv. Allee. Meloidogyne arenaria and M. javanica were identified from U. parvifolia cv. Drake. This is a first report of t(..)

DOI: 10.21307/jofnem-2020-049

Detoxification-related gene expression accompanies anhydrobiosis in the foliar nematode (Aphelenchoides fragariae)

Zhen Fu/ Paula Agudelo/ Christina E. Wells

Abstract The foliar nematode (Aphelenchoides fragariae) is a quarantined pest that infects a broad range of herbaceous and woody plants. Previous work has demonstrated its remarkable ability to survive rapid and extreme desiccation, although the specific molecular mechanisms underlying its anhydrobiotic response have not been characterized. The authors used RNA sequencing and de novo transcriptome assembly to compare patterns of gene expression between hydrated and 24-hr desiccated nematodes. In(..)

DOI: 10.21307/jofnem-2020-047

Morphological and molecular characterization of Ektaphelenchoides pini (Massey, 1966) Baujard, 1984 (Aphelenchoididae; Ektaphelenchinae) from Iran, with morphological and taxonomic observations on some species

Fariba Heydari/ Majid Pedram

Abstract Ektaphelenchoides pini, the type species of the genus Ektaphelenchoides, was recovered from wood and bark samples of a dead broadleaf forest tree collected from the forests of Golestan province in north of Iran. The recovered population is mainly characterized by massive wide spicules of males with well-developed condylus marked by indentations at the apex and simple distal tip. It was further characterized by 756 to 947 μm long females having a cephalic region slightly separated from t(..)

DOI: 10.21307/jofnem-2020-052

Description and molecular phylogeny of Mesocriconema abolafiai n. sp. (Nematoda: Criconematidae) from Iran

Hossein Mirbabaei Karani/ Ali Eskandari/ Reza Ghaderi/ Akbar Karegar

Abstract Mesocriconema abolafiai n. sp. is described by morphological, morphometric, and molecular approaches. The new species is characterized by a body slightly curved with 402 to 612 μm length, 90 to 113 cuticular body annuli with smooth to irregular margins lacking of crenation with not more than one anastomoses, lip region not offset, small flattened submedian lobes, stylet robust (52.8-60.0 µm) with well-developed knobs, open vulva with simple anterior lip, straight vagina, filled spermath(..)

DOI: 10.21307/jofnem-2020-048