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Journal of Nematology

Society of Nematologists

Subject: Life Sciences


ISSN: 0022-300X
eISSN: 2640-396X



Enhanced entomopathogenic nematode yield and fitness via addition of pulverized insect powder to solid media
Nematotoxic coumarins from Angelica pubescens Maxim. f. biserrata Shan et Yuan roots and their physiological effects on Bursaphelenchus xylophilus
Broad-based root-knot nematode resistance identified in cowpea gene-pool two
Description of Pseudacrobeles (Pseudacrobeles) curvatus sp. n. (Cephalobidae: Rhabditida) in South Korea

VOLUME 52 (2020) - List of articles

Control of the tomato leaf miner, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) larvae in laboratory using entomopathogenic nematodes from subtropical environment

Bonginkhosi E. Dlamini/ Nelisiwe Dlamini/ Michael T. Masarirambi/ Nxumalo Kwanele A.

Abstract Tomato (Solanum esculentum) is one of the vegetable crops grown by both smallholder and commercial farmers in the Kingdom of Eswatini. Tomato leaf miner, Tuta absoluta M. is a major insect pest of tomatoes resulting in reduced tomato yields throughout the country. The study investigated the virulence of two sub-tropical EPN species on T. absoluta larvae. Steinernema yirgalemense and S. jeffreyense at different concentrations (0, 20, 40, 60 IJs/insect) were screened for efficacy (i.e. mo(..)

DOI: 10.21307/jofnem-2020-013

First report of Meloidogyne enterolobii infecting Japanese blue berry tree (Elaeocarpus decipiens) in Florida, USA

M. R. Moore/ J. A. Brito/ S. Qiu/ C. G. Roberts/ L. A. Combee

Abstract In October 2019, samples of galled roots with rhizosphere soil were collected from declining Elaeocarpus decipiens in Hernando County, Florida. Extracted root-knot nematodes were identified by both molecular and morphological methods as Meloidogyne enterolobii. This is a first report of this regulated root-knot nematode on Elaeocarpus decipiens in Florida.

DOI: 10.21307/jofnem-2020-005

The effects of Meloidogyne incognita and Heterodera glycines on the yield and quality of edamame (Glycine max l.) in Arkansas

J. E. Wilkes/ T. L. Kirkpatrick

Abstract In 2012, the first domestic commercial edamame processing plant was established in Arkansas and edamame production was contracted out to local growers. Although the state is a major soybean producer, studies of nematode effects on edamame are limited. A survey of nematode genera and density in 64 contracted edamame production fields was conducted in 2013 and 2014. In both years, Meloidogyne and Heterodera were present in less than half of the surveyed fields while Pratylenchus was the m(..)

DOI: 10.21307/jofnem-2020-012

Fungal communities associated with Heterodera glycines and their potential in biological control: a current update

Deepak Haarith/ Kathryn E. Bushley/ Senyu Chen

Abstract The soybean cyst nematode (SCN) is the most important pest on soybean, a major crop worldwide. The SCN is considered both parasitic and pathogenic as it derives nutrition from the host and manipulates host physiology to do so. Currently, there are no commercially available chemicals that are specific, environmentally safe and cost effective to control SCN levels. Crop rotation, use of host resistance and other cultural practices remain the main management strategies. The need for biopro(..)

DOI: 10.21307/jofnem-2020-022

Genome sequence of the root-knot nematode Meloidogyne luci

Nik Susič/ Georgios D. Koutsovoulos/ Cristian Riccio/ Etienne G. J. Danchin/ Mark L. Blaxter/ David H. Lunt/ Polona Strajnar/ Saša Širca/ Gregor Urek/ Barbara Gerič Stare

Abstract Root-knot nematodes from the genus Meloidogyne are polyphagous plant endoparasites and agricultural pests of global importance. Here, we report the high-quality genome sequence of Meloidogyne luci population SI-Smartno V13. The resulting genome assembly of M. luci SI-Smartno V13 consists of 327 contigs, with an N50 contig length of 1,711,905 bp and a total assembly length of 209.16 Mb.

DOI: 10.21307/jofnem-2020-025

A draft genome of a field-collected Steinernema feltiae strain NW

Zhen Fu/ Yuxiang Li/ Axel A. Elling/ William E. Snyder

Abstract Advances in sequencing technologies have accelerated our understanding of the complex genetic network of organisms and genomic divergences that are linked to evolutionary processes. While many model organisms and laboratory strains have been sequenced, wild populations are underrepresented in the growing list of sequenced genomes. Here, we present a de novo assembly of Steinernema feltiae, strain NW, collected from a working agricultural field in south central Washington, USA. Leveragin(..)

DOI: 10.21307/jofnem-2020-003

Molecular approach to confirm traditional identification of Radopholus similis sampled in Tanzania

Doreen M. Mgonja/ Gladness E. Temu/ Joseph C. Ndunguru/ Magreth F. Mziray/ Sylvester L. Lyantagaye/ Nessie D. Luambano

Abstract Banana (Musa spp. L.) is an important staple food and cash crop for about 30% of the population in Tanzania; however, the burrowing plant-parasitic nematode Radopholus similis causes black head disease and toppling in banana plants, which results in yield losses. We collected and identified 80 specimens of R. similis from four agro-ecological zones in Tanzania using morphological characters. We then used universal and specific R. similis primers to amplify the small subunit, internal tr(..)

DOI: 10.21307/jofnem-2020-020

Silver nanoparticles as a potential nematicide against Meloidogyne graminicola

Richa Baronia/ Puneet Kumar/ S. P. Singh/ R. K. Walia

Abstract Plant-parasitic nematodes cause severe damage to the various agricultural crops, leading to economic losses for farmers. Therefore, identification and development of novel and environmentally benign nematicides is critically important. In this study, a silver nanoparticle (AgNP) formulation was synthesized, characterized, and investigated as a potential nematicide against rice root-knot nematode, Meloidogyne gramnicola, on rice (Oryza sativa). A series of lab assays (water and sand scre(..)

DOI: 10.21307/jofnem-2020-002

Description of Laimaphelenchus sinensis n. sp. (Nematoda: Aphelenchoididae) from declining Chinese pine, Pinus tabuliformis in Beijing, China

Jianfeng Gu/ Munawar Maria/ Yiwu Fang/ Lele Liu/ Yong Bian/ Xianfeng Chen

Abstract Laimaphelenchus sinensis n. sp. isolated from declining Chinese pine, Pinus tabuliformis Carrière, is described and characterized morphologically and molecularly. The new species has four incisures in the lateral field and the excretory pore situated posterior to the nerve ring; the female has a vulval flap and vaginal sclerotization is quite prominent in majority of specimens. The female tail is conoid, ventrally curved having a single stalk-like terminus with 8 to 10 projections. The (..)

DOI: 10.21307/jofnem-2020-019

Molecular and morphological characterization of the alfalfa cyst nematode, Heterodera medicaginis, from Utah

Zafar A. Handoo/ Andrea M. Skantar/ Saad L. Hafez/ Mihail R. Kantor/ Maria N. Hult/ Stephen A. Rogers

Abstract In the spring of 2019, a cyst nematode was discovered from soil samples collected from an alfalfa field in Millard County, Utah. The soil samples were submitted to one of us (SH), who extracted the nematode cysts and sent them to the USDA-ARS, Mycology and Nematology Genetic Diversity and Biology Laboratory (MNGDBL), Beltsville, MD for morphological and molecular identification. Cysts and living nematode juveniles (J2) recovered from the cysts were examined morphologically and molecular(..)

DOI: 10.21307/jofnem-2020-015

Improvement of long segment ribosomal PCR amplification for molecular identification of Litylenchus crenatae mccannii associated with beech leaf disease

L.K. Carta/ S. Li

Abstract Generating DNA markers for microscopic plant parasitic nematodes can be especially difficult if only a few valuable, tiny specimens are available. Providing a reliable maximum amount of unambiguous genetic information from single nematodes is especially important when identifying damaging, regulated nematodes of importance to trade where a few nucleotide differences in diagnostic markers are significant. There are many possible reasons for difficulty amplifying unpurified nematode DNA f(..)

DOI: 10.21307/jofnem-2020-016

Essential oils for managing Pratylenchus penetrans on Easter lilies

B. B. Westerdahl/ D. Giraud/ L. J. Riddle/ C. A. Anderson

Abstract Easter lily bulbs for greenhouse forcing are produced in Del Norte County, California and Curry County, Oregon, USA. Pratylenchus penetrans infestation seriously affects growth of field grown bulbs. During two consecutive years of field trials containing 22 treatments, commercially prepared formulations of essential oils (EOs) were compared to an untreated control and to a standard chemical fumigant treatment (FU) (1,3-dichloropropene and metam sodium) applied preplant followed by phora(..)

DOI: 10.21307/jofnem-2020-010

Characterization of root-knot nematodes infecting mulberry in Southern China

Pan Zhang/ Hudie Shao/ Chunping You/ Yan Feng/ Zhenwen Xie

Abstract China is one of the largest producers of mulberry in the world. With the development of the sericulture industry, several pests and diseases have occurred in rapid succession, chief among which is the root-knot nematode disease affecting mulberry. According to the China cocoon and silk exchange, cocoon prices have doubled since the beginning of 2009 and rose to 92,700 yuan ($135,770) per tonne in mid-April 2010. According to customs statistics, in the first eight months of 2011, China’s(..)

DOI: 10.21307/jofnem-2020-004

First report of a stunt nematode Tylenchorhynchus zeae on corn in Gansu Province, China

Zhi Peng Xu/ Hui Xia Li/ Yong Gang Liu/ Bao Cang Ren/ Chun Hui Ni/ Jin Hui Ma

Abstract During a survey of plant parasitic nematodes in 2019, at Gansu Province, China, the stunt nematode Tylenchorhynchus zeae was found parasitizing corn seedlings. Females, males and juveniles of T. zeae were observed on soil and roots samples of corn after processing and extraction. This population of stunt nematodes was identified based on morphological and by sequencing the ITS1 region of rDNA and D2/D3 fragments of the 28 S rRNA. The ITS1 and the D2/D3 sequences of this population, shar(..)

DOI: 10.21307/jofnem-2020-009

First report of root-knot nematode, Meloidogyne arenaria, on lavender in Turkey

Tevfik Özalp/ Gonca Könül/ Önder Ayyıldız/ Adnan Tülek/ Zübeyir Devran

Abstract Lavender is a medicinal and aromatic plant that is widely grown in Turkey. Gall symptoms were observed on roots of lavender (Lavandula angustifolia Mill.) collected from Kırklareli and Edirne provinces. Egg masses were collected from galled roots. DNA isolated from all samples was screened by species-specific primers belonging to the most common species of root-knot nematodes and M. arenaria was the only species that was identified in all of the samples analyzed. This is the first repor(..)

DOI: 10.21307/jofnem-2020-008

Description of Seinura italiensis n. sp. (Tylenchomorpha: Aphelenchoididae) found in the medium soil imported from Italy

Jianfeng Gu/ Munawar Maria/ Lele Liu/ Majid Pedram

Abstract Seinura italiensis n. sp. isolated from the medium soil imported from Italy is described and illustrated using morphological and molecular data. The new species is characterized by having short body (477 (407-565) µm and 522 (469-590) µm for males and females, respectively), three lateral lines, stylet lacking swellings at the base, and excretory pore at the base or slightly anterior to base of metacorpus; females have 58.8 (51.1-69.3) µm long post-uterine sac (PUS), elongate conical ta(..)

DOI: 10.21307/jofnem-2020-018

Soil texture, infective juvenile concentration, and soil organic matter influence the efficacy of Steinernema feltiae isolate Lican Ray

Gabriela Lankin/ Giselle Vidal-Retes/ Geraldine Allende/ Carlos Castaneda-Alvarez/ Ernesto San-Blas/ Erwin Aballay

Abstract The influence of infective juveniles (IJs) concentration, soil texture, IJ-host distance and organic matter (OM) content, at different decomposition degree, on the activity of the nematode Steinernema feltiae isolate Lican Ray (LR) was examined using Galleria mellonella larvae. Bioassays were conducted in tubes of varied length, filled with soil of different textures, placed either vertically or horizontally. In the concentration assay, highest IJ concentrations caused maximum larval mo(..)

DOI: 10.21307/jofnem-2020-007

A new rare nematode Nothocriconemoides hangzhouensis n. sp. (Nematoda: Criconematidae) from Hangzhou, China

Munawar Maria/ Wentao Miao/ Ruihang Cai/ Pablo Castillo/ Jingwu Zheng

Abstract The Family Criconematidae is commonly referred as ring nematodes that include some members with economic importance as plant parasites. During a recent nematode inventory survey at Zhejiang Province, China, a new species of genus Nothocriconemoides was detected in the rhizosphere of elm tree. Nothocriconemoides hangzhouensis n. sp. can be characterized by the female body having annuli with fine longitudinal striations and 2 to 3 anastomoses at the posterior half of the body. The first c(..)

DOI: 10.21307/jofnem-2020-006

Transcriptomic analysis of Bursaphelenchus xylophilus treated by a potential phytonematicide, punicalagin

Qun-Qun Guo/ Gui-Cai Du/ Ting-Ting Zhang/ Mei-Juan Wang/ Chao Wang/ Hong-Tao Qi/ Rong-Gui Li

Abstract Punicalagin showed significant nematotoxic activity against pine wood nematode (PWN), Bursaphelenchus xylophilus, in the authors’ previous research. The authors performed high-throughput transcriptomic sequencing of punicalagin-treated nematodes to generate clues for its nematotoxic mechanism of action. The authors identified 2,575 differentially expressed genes, 1,428 of which were up-regulated and 1,147 down-regulated. Based on a comprehensive functional in silico analysis, the author(..)

DOI: 10.21307/jofnem-2020-001

Natural occurrence and distribution of entomopathogenic nematodes (Steinernematidae, Heterorhabditidae) in Viti Levu, Fiji Islands

Gilianne Brodie

Abstract In Fiji, little or no attention has been given to entomopathogenic nematodes (EPNs) in biocontrol programs due to the lack of awareness about their occurrence and distribution in Fiji. A survey of EPNs was conducted for the first time in Fiji Islands in 2012 and 2013, throughout the eight provinces in Viti Levu to determine the occurrence and distribution across different habitat in Viti Levu. The soil samples from various habitats were collected and assayed for the presence of EPN usin(..)

DOI: 10.21307/jofnem-2020-017

Effect of an Alltech soil health product on entomopathogenic nematodes, root-knot nematodes and on the growth of tomato plants in the greenhouse

Anusha Pulavarty/ Karina Horgan/ Thomais Kakouli-Duarte

Abstract An organic product that consists of proprietary blend of fermentation and plant extracts with micronutrients (ACS 5075, Alltech®, Inc., Nicholasville, KY USA) was evaluated against four strains of entomopathogenic nematodes (EPN): Steinernema feltiae (SB12(1), a wild enviroCORE strain and a commercial form e-NEMA), Steinernema carpocapsae (e-NEMA), and Heterorhabditis bacteriophora. The effects on egg hatching and survival of root-knot nematodes (RKN) were also examined. The sensitivity(..)

DOI: 10.21307/jofnem-2020-014

Elimination of Tobacco rattle virus from viruliferous Paratrichodorus allius in greenhouse pot experiments through cultivation of castle russet

Richard A. Quick/ Launa Cimrhakl/ Hassan Mojtahedi/ Vidyasagar Sathuvalli/ Maximilian J. Feldman/ Charles R. Brown

Abstract Corky ringspot (CRS) is a widespread potato tuber necrotic disease caused by Tobacco rattle virus (TRV) infection. In the Pacific Northwest, this virus is transmitted by the stubby root nematode (SRN) within the genus Paratrichodorus. Remediating CRS affected fields is a major challenge that can be mitigated by growing plant varieties that are resistant to TRV infection. Growing alfalfa has been shown to reduce TRV levels in CRS infested fields over time but the development of a potato (..)

DOI: 10.21307/jofnem-2020-011

Characterization of Vittatidera zeaphila (Nematoda: Heteroderidae) from Indiana with molecular phylogenetic analysis of the genus

Andrea M. Skantar/ Zafar A. Handoo/ Mihail R. Kantor/ Lynn K. Carta/ Jamal Faghihi/ Virginia Ferris

Abstract In the summer of 2016, a field of corn (Zea mays) in Spencer County, Indiana was observed with heavily stunted plants, and from the affected roots a large number of cysts were recovered. Soil samples were submitted to one of us (JF), who extracted the nematode cysts and sent them to the USDA-ARS, Mycology and Nematology Genetic Diversity and Biology Laboratory (MNGDBL), Beltsville, MD for morphological and molecular identification. Cysts and the recovered second-stage juveniles (J2) tha(..)

DOI: 10.21307/jofnem-2020-024

First report of the sting nematode Belonolaimus longicaudatus infecting bermudagrass in Barbados

P. Mc Groary/ W. Ye/ E. Nangle

Abstract In 2016, “Tifdwarf” hybrid bermudagrass (Cynodon dactylon (L) Pers. × C. transvaalensis Burtt-Davy) grown on a golf green built to the United States Golf Association recommendations in Barbados started to show irregular significant chlorotic patches followed by gradual thinning and decline of turfgrass. A survey was conducted in May 2016 to determine the presence of plant-parasitic nematodes. The results revealed the presence of the plant-parasitic sting nematode Belonolaimus longicauda(..)

DOI: 10.21307/jofnem-2020-021

First report of Bursaphelenchus fungivorus (Nematoda: Aphelenchida) in Italy and an overview of nematodes associated with Crocus sativus L.

Giulia Torrini/ Agostino Strangi/ Stefania Simoncini/ Maria Luppino/ Pio Federico Roversi/ Leonardo Marianelli

Abstract Saffron is one of the most precious spices in the world. It is derived from the dried stigmas of the Crocus sativus L. flowers. This plant is triploid sterile and propagated by corms. As a subterranean organ, the corm can come into contact with different nematodes. In this contribution Bursaphelenchus fungivorus was reported for the first time in Italy. It was found associated with C. sativus corms and characterized based on morphological and morphometrical characteristics for this spec(..)

DOI: 10.21307/jofnem-2020-023

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