Memorial Tribute

DOI: 10.33073/pjm-2020-040

Yeasts Associated with Various Amazonian Native Fruits

CARLOS VEGAS/ AMPARO I. ZAVALETA/ PAMELA E. CANALES/ BRAULIO ESTEVE-ZARZOSO

Abstract Yeasts, commonly present on the surface of fruits, are of industrial interest for the production of enzymes, flavorings, and bioactive compounds, and have many other scientific uses. The Amazonian rainforest may be a good source of new species or strains of yeasts, but their presence on Amazonian fruits is unknown. The aim of this study was to identify and characterize yeasts isolated from Amazonian native fruits using molecular and phenotypic methods. In total, 81 yeast isolates were o(..)

DOI: 10.33073/pjm-2020-027

Published Online: 05-August-2020

Molecular Identification and Prevalence of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii in Erbil City, Northern Iraq

SHLER AKRAM FAQE MAHMOOD/ HAWRI MUSTAFA BAKR

Abstract The present study was conducted to evaluate the infection rates of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii among asymptomatic individuals in Erbil City, northern Iraq. The research intent was to discover whether pathogenic or nonpathogenic species cause a high rate of symptomless Entamoeba infections. Stool samples were microscopically examined, and the 18S-rRNA gene was targeted utilizing the nested PCR technique in the positive specimens. Initial results bas(..)

DOI: 10.33073/pjm-2020-028

Published Online: 05-August-2020

Improved Biosurfactant Production by Enterobacter cloacae B14, Stability Studies, and its Antimicrobial Activity

JINDARAT EKPRASERT/ SASIWIMON KANAKAI/ SULADDA YOSPRASONG

Abstract This work aimed to optimize carbon and nitrogen sources for the growth of Enterobacter cloacae B14 and its biosurfactant (BS) production via One-Variable-At-a-Time (OVAT) method. The BS stability under a range of pH and temperatures was assessed. Antimicrobial activity against Gram-positive and Gram-negative pathogens was determined by the agar well diffusion method. The results showed that the optimum carbon and nitrogen sources for BS production were maltose and yeast extract, respect(..)

DOI: 10.33073/pjm-2020-030

Published Online: 14-August-2020

Characteristics and Diversity of Endophytic Bacteria in Endangered Chinese Herb Glehnia littoralis Based on Illumina Sequencing

XIAOWEI HUO/ YUE WANG/ DAWEI ZHANG/ TING GAO/ MENGMENG LIU

Abstract Glehnia littoralis is an endangered medicinal plant growing in the coastal ecological environment and plays an important role in coastal ecosystems. The endophytes in the plant have a significant role in promoting plant growth and enhancing plant stress resistance. However, the endophytic bacterial structure associated with halophyte G. littoralis is still not revealed. In this project, the construction and diversity of endophytic bacterial consortium associated with different tissues o(..)

DOI: 10.33073/pjm-2020-031

Published Online: 08-September-2020

Establishment and Application of a Dual TaqMan Real-Time PCR Method for Proteus Mirabilis and Proteus Vulgaris

RUI YANG/ GUOYANG XU/ XIAOYOU WANG/ ZHICHU QING/ LIZHI FU

Abstract Proteus species are common opportunistic bacteria and foodborne pathogens. The proper detection of Proteus can effectively reduce the occurrence of food-borne public health events. Proteus mirabilis and Proteus vulgaris are the two most important pathogens in the Proteus genus. In this study, a dual TaqMan Real-Time PCR method was established to simultaneously detect and distinguish P. mirabilis and P. vulgaris in samples. The method exhibited good specificity, stability, and sensitivit(..)

DOI: 10.33073/pjm-2020-032

Published Online: 25-August-2020

The Effect of Environmental Stresses on lipL32 Gene Expression in Pathogenic Leptospira spp. through Real-Time PCR

SONA ROSTAMPOUR YASOURI/ MONIR DOUDI/ MASOOD GHANE/ NAFISEH SADAT NAGHAVI/ ABOLHASAN REZAEI

Abstract Leptospirosis is a worldwide infectious and zoonotic disease. The incidence of this disease is high in temperate regions, especially in northern Iran. The aim of this study was to investigate the effects of temperature, pH, and Phyllanthus amarus plant extract on the lipL32 gene expression in pathogenic Leptospira spp. Fifty water samples were collected. Culture and PCR technique were used to isolate and identify the bacterium and the presence of the lipL32 gene. The samples were expose(..)

DOI: 10.33073/pjm-2020-033

Published Online: 08-September-2020

A Polyclonal Spread Emerged: Characteristics of Carbapenem-Resistant Klebsiella pneumoniae Isolates from the Intensive Care Unit in a Chinese Tertiary Hospital

ZHENGZHENG WANG/ FANGYOU YU/ XIAOFEI SHEN/ MEILAN LI

Abstract Carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates often cause nosocomial infections with limited therapeutic options and spread rapidly worldwide. In this study, we revealed a polyclonal emergence of CRKP isolates from the intensive care unit in a Chinese tertiary hospital. We applied a series of methods including automated screening, antimicrobial susceptibility testing, the modified carbapenem inacti vation method (mCIM), PCR amplification, DNA sequencing, and multilocus sequ(..)

DOI: 10.33073/pjm-2020-034

Published Online: 08-September-2020

Marine Sediment Recovered Salinispora sp. Inhibits the Growth of Emerging Bacterial Pathogens and other Multi-Drug-Resistant Bacteria

LUIS CONTRERAS-CASTRO/ SERGIO MARTÍNEZ-GARCÍA/ JUAN C. CANCINO-DIAZ/ LUIS A. MALDONADO/ CLAUDIA J. HERNÁNDEZ-GUERRERO/ SERGIO F. MARTÍNEZ-DÍAZ/ BÁRBARA GONZÁLEZ-ACOSTA/ ERIKA T. QUINTANA

Abstract Marine obligate actinobacteria produce a wide variety of secondary metabolites with biological activity, notably those with antibiotic activity urgently needed against multi-drug-resistant bacteria. Seventy-five marine actinobacteria were isolated from a marine sediment sample collected in Punta Arena de La Ventana, Baja California Sur, Mexico. The 16S rRNA gene identification, Multi Locus Sequence Analysis, and the marine salt requirement for growth assigned seventy-one isolates as mem(..)

DOI: 10.33073/pjm-2020-035

Published Online: 08-September-2020

Determining Risk Factors for Dengue Fever Severity in Jeddah City, a Case-Control Study (2017)

WAJD A. ABUALAMAH/ HUSSAIN S. BANNI/ HUSSAIN A. ALMASMOUM/ YONIS A. ALLOHIBI/ HASAN M. SAMARIN/ MOHAMMED A. BAFAIL

Abstract Dengue fever is a major public health problem in Saudi Arabia. Unfortunately, preventive strategies are still deficient. It can progress to severe and lethal forms, and available knowledge does not allow early prediction of which cases of dengue fever (DF) will progress to dengue hemorrhagic fever (DHF). The aim of this study was to evaluate the host and viral factors that could play a role in the progression of severe dengue cases in the frame of the revised 2009 WHO classification. Da(..)

DOI: 10.33073/pjm-2020-036

Published Online: 26-August-2020

Characterization of Ligninolytic Bacteria and Analysis of Alkali-Lignin Biodegradation Products

YI XIONG/ YARU ZHAO/ KUIKUI NI/ YUE SHI/ QINGFANG XU

Abstract Ligninolytic bacteria degrading lignin were isolates and identified, and their biodegradation mechanism of alkaline-lignin was investigated. Four strains with lignin degradation capability were screened and identified from the soil, straw, and silage based on their decolorizing capacity of aniline blue and colony size on alkaline-lignin medium. The degradation ratio of Bacillus aryabhattai BY5, Acinetobacter johnsonii LN2, Acinetobacter lwoffii LN4, and Micrococcus yunnanensis CL32 have(..)

DOI: 10.33073/pjm-2020-037

Published Online: 08-September-2020

Characterization of Two Macrolide Resistance-Related Genes in Multidrug-Resistant Pseudomonas aeruginosa Isolates

QING CHEN/ WEI LU/ DANYING ZHOU/ GUOTONG ZHENG/ HONGMAO LIU/ CHANGRUI QIAN/ WANGXIAO ZHOU/ JUNWAN LU/ LIYAN NI/ QIYU BAO/ AIFANG LI/ TENG XU/ HAILI XU

Abstract In analyzing the drug resistance phenotype and mechanism of resistance to macrolide antibiotics of clinical Pseudomonas aeruginosa isolates, the agar dilution method was used to determine the minimum inhibitory concentrations (MICs), and PCR (polymerase chain reaction) was applied to screen for macrolide antibiotics resistance genes. The macrolide antibiotics resistance genes were cloned, and their functions were identified. Of the 13 antibiotics tested, P. aeruginosa strains showed hig(..)

DOI: 10.33073/pjm-2020-038

Published Online: 08-September-2020

Isolated Phosphate-Solubilizing Soil Bacteria Promotes In vitro Growth of Solanum tuberosum L.

GUSTAVO YAÑEZ-OCAMPO/ MARTHA E. MORA-HERRERA/ ARNOLDO WONG-VILLARREAL/ DENISSE M. DE LA PAZ-OSORIO/ NADIA DE LA PORTILLA-LÓPEZ/ JORGE LUGO/ ROCIO VACA-PAULÍN/ PEDRO DEL ÁGUILA

Abstract The capacity of four bacterial strains isolated from productive soil potato fields to solubilize tricalcium phosphate on Pikovskaya agar or in a liquid medium was evaluated. A bacterial strain was selected to evaluate in vitro capacity of plant-growth promotion on Solanum tuberosum L. culture. Bacterial strain A3 showed the highest value of phosphate solubilization, reaching a 20 mm-diameter halo and a concentration of 350 mg/l on agar and in a liquid medium, respectively. Bacterial str(..)

DOI: 10.33073/pjm-2020-039

Published Online: 08-September-2020

Characteristics of the Jejunal Microbiota in 35-Day-Old Saba and Landrace Piglets

HUAN GAO/ YUTING YANG/ ZHENHUI CAO/ JINMING RAN/ CHUNYONG ZHANG/ YING HUANG/ MINGHUA YANG/ SUMEI ZHAO/ QINGCONG AN/ HONGBIN PAN

Abstract The balanced microbiological system is a significant hallmark of piglet health. One of the crucial factors affecting intestinal microbiota is the host’s genetics. This study explored the difference in the diversity of jejunal microbiota between Saba (SB) and Landrace (LA) piglets. Nine Saba and nine Landrace piglets were fed with sow’s milk until day 35. Jejunal contents were harvested for 16S rRNA sequencing. The birth weight, body weight, and average daily gain of Saba piglets were lo(..)

DOI: 10.33073/pjm-2020-041

Published Online: 08-September-2020

In vitro Antagonistic Activity of Endophytic Fungi Isolated from Shirazi Thyme (Zataria multiflora Boiss.) against Monosporascus cannonballus

RAHIL SAID AL-BADI/ THAMODINI GAYA KARUNASINGHE/ ABDULLAH MOHAMMED AL-SADI/ ISSA HASHIL AL-MAHMOOLI/ RETHINASAMY VELAZHAHAN

Abstract Endophytic fungi viz., Nigrospora sphaerica (E1 and E6), Subramaniula cristata (E7), and Polycephalomyces sinensis (E8 and E10) were isolated from the medicinal plant, Shirazi thyme (Zataria multiflora). In in vitro tests, these endophytes inhibited the mycelial growth of Monosporascus cannonballus, a plant pathogenic fungus. Morphological abnormalities in the hyphae of M. cannonballus at the edge of the inhibition zone in dual cultures with N. sphaerica were observed. The culture filtr(..)

DOI: 10.33073/pjm-2020-029

Published Online: 05-August-2020