An evaluation of the antibacterial properties and shear bond strength of copper nanoparticles as a nanofiller in orthodontic adhesive


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Australasian Orthodontic Journal

Australian Society of Orthodontists

Subject: Dentistry, Orthodontics & Medicine


ISSN: 2207-7472
eISSN: 2207-7480





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VOLUME 31 , ISSUE 1 (May 2015) > List of articles

An evaluation of the antibacterial properties and shear bond strength of copper nanoparticles as a nanofiller in orthodontic adhesive

Liliana Argueta-Figueroa / Rogelio J. Scougall-Vilchis * / Raúl A. Morales-Luckie / Oscar F. Olea-Mejía

Citation Information : Australasian Orthodontic Journal. Volume 31, Issue 1, Pages 42-48, DOI:

License : (CC BY 4.0)

Published Online: 15-August-2021



Objectives: To evaluate the antibacterial properties and effects of an orthodontic adhesive containing copper nanoparticles (NPs) on the material’s shear bond strength.

Methods: Antimicrobial activity was analysed by a disk diffusion test against S. aureus, E. coli and S. mutans. The NPs were added to the orthodontic adhesive at 0.0100 wt%, 0.0075 wt%, and 0.0050 wt%. Sixty extracted bicuspids were divided into two groups and the enamel of all teeth was conditioned with phosphoric acid. A coat of moisture insensitive primer (MIP) was applied prior to the bonding of brackets with composite resin. Group I served as a control and the bonding procedure was performed according to the manufacturer’s instructions. Group II comprised the test teeth, into which 0.0100 wt% copper NPs were included in the MIP. Samples were tested and statistically analysed (p ≤ 0.05). The adhesive remnant index (ARI) was also assessed microscopically.

Results: The adhesive with copper NPs showed a bactericidal effect against the bacteria under study. A significantly higher bond strength was obtained with the orthodontic adhesive that included 0.0100 wt% of copper NPs (15.23 ± 6.8 MPa) in comparison with the control group (9.59 ± 4.3 MPa). The ARI scores indicated that the groups were significantly different and strengthened by the incorporation of NPs (p = 0.004).

Conclusion: The results of the present study suggested that an orthodontic adhesive, which included copper NPs, significantly increased material shear bond strength without adverse side effects on colour and appearance. The adhesive interface was strengthened by homogeneously dispersed copper NPs added as a nanofiller.

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1. Scougall-Vilchis RJ, Zárate-Días C, Kusakabe S, Yamamoto K. Bond strengths of different orthodontic adhesives after enamel conditioning with the same self-etching primer. Aust Orthod J 2010;26:84-9.

2. Buck T, Pellegrini P, Sauerwein R, Leo MC, Covell DA Jr, Maier T et al. Elastomeric-ligated vs self-ligating appliances: a pilot study examining microbial colonization and white spot lesion formation after 1 year of orthodontic treatment. Orthodontics (Chic.) 2011;12:108-21.

3. Sudjalim TR, Woods MG, Manton DJ. Prevention of white spot lesions in orthodontic practice: a contemporary review. Aust Dent J 2006;51:284-9.

4. Mobarak EH, El-Korashy DI, Pashley DH. Effect of chlorhexidine concentrations on micro-shear bond strength of self-etch adhesive to normal and caries-affected dentin. Am J Dent 2010;23:217-22.

5. Frey C, Yetkiner E, Stawarczyk B, Attin T, Attin R. Effects of different chlorhexidine pretreatments on adhesion of metal brackets in vitro. Head Face Med 2012;8:36.

6. Rai A, Prabhune A, Perry CC. Antibiotic mediated synthesis of gold nanoparticles with potent antimicrobial activity and their application in antimicrobial coatings. J Mat Chem 2010;20:6789-98.

7. Xiu ZM, Zhang QB, Puppala HL, Colvin VL, Alvarez PJ. Negligible particle-specific antibacterial activity of silver nanoparticles. Nano Lett 2012;12:4271-5.

8. Eshed M, Lellouche J, Matalon S, Gedanken A, Banin E. Sonochemical coatings of ZnO and CuO nanoparticles inhibit Streptococcus mutans biofilm formation on teeth model. Langmuir 2012;28:12288-95.

9. Ren G, Hu D, Cheng EW, Vargas-Reus MA, Reip P, Allaker RP. Characterisation of copper oxide nanoparticles for antimicrobial applications. Int J Antimicrob Agents 2009;33:587-90.

10. Esteban-Cubillo A, Pecharromán C, Aguilar E, Santarén J, Moya JS. Antibacterial activity of copper monodispersed nanoparticles into sepiolite. J Mater Sci 2006;41:5208-12.

11. Cioffi N, Torsi L, Ditaranto N, Tantillo G, Ghibelli L, Sabbatini L et al. Copper nanoparticle/polymer composites with antifungal and bacteriostatic properties. Chem Mater 2005;17:5255-62.

12. Gabbay J, Borkow G, Mishal J, Magen E, Zatcoff R, Shemer-Avni Y. Copper oxide impregnated textiles with potent biocidal activities. J Ind Text 2006;35:323-35.

13. Jadhav S, Gaikwad S, Nimse M, Rajbhoj A. Copper oxide nanoparticles: synthesis, characterization and their antibacterial activity. J Clust Sci 2011;22:121-9.

14. Raffi M, Mehrwan S, Bhatti TM, Akhter JI, Hameed A, Yawar W et al. Investigations into the antibacterial behavior of copper nanoparticles against Escherichia coli. Ann Microbiol 2010;60:75-80.

15. Borkow G, Gabbay J. Putting copper into action: copperimpregnated products with potent biocidal activities. FASEB J 2004;18:1728-30.

16. Chatterjee AK, Sarkar RK, Chattopadhyay AP, Aich P, Chakraborty R, Basu T. A simple robust method for synthesis of metallic copper nanoparticles of high antibacterial potency against E. coli. Nanotechnology 2012;23:085103.

17. Ruparelia JP, Chatterjee AK, Duttagupta SP, Mukherji S. Strain specificity in antimicrobial activity of silver and copper nanoparticles. Acta Biomater 2008;4:707-16.

18. Argueta-Figueroa L, Morales-Luckie RA, Scougall-Vilchis RJ, OleaMejía OF. Synthesis, characterization and antibacterial activity of copper, nickel and bimetallic Cu-Ni nanoparticles for potential use in dental materials. Prog Nat Sci: Mat Int 2014;24:321-8.

19. Wayne P. Performance standards for antimicrobial disk susceptibility tests. Nineteenth informational supplement M100-S19. Clinical and Laboratory Standards Institute 2009.

20. Årtun J, Bergland S. Clinical trials with crystal growth conditioning as an alternative to acid-etch enamel pretreatment. Am J Orthod 1984;85:333-40.

21. Pandis N, Papaioannou W, Kontou E, Nakou M, Makou M, Eliades T. Salivary Streptococcus mutans levels in patients with conventional and self-ligating brackets. Eur J Orthod 2010;32:94-9.

22. Paes Leme Af, Koo H, Bellato CM, Bedi G, Cury JA. The role of sucrose in cariogenic dental biofilm formation – new insight. J Dent Res 2006;85:878-87.

23. Tufekci E, Dixon JS, Gunsolley JC, Lindauer SJ. Prevalence of white spot lesions during orthodontic treatment with fixed appliances. Angle Orthod 2011;81:206-10.

24. Meguid SA, Sun Y. On the tensile and shear strength of nanoreinforced composite interfaces. Materials & design 2004;25:289-96.

25. Paul DR, Robeson LM. Polymer nanotechnology: nanocomposites. Polymer 2008;49:3187-204.

26. Cozza P, Martucci L, De Toffol L, Penco SI. Shear bond strength of metal brackets on enamel. Angle Orthod 2006;76:851-6.

27. Mackay ME, Tuteja A, Duxbury PM, Hawker CJ, Van Horn B, Guan Z et al. General strategies for nanoparticle dispersion. Science 2006;311:1740-3.