Susceptibility of Flordaguard peach rootstock to a resistant-breaking population of Meloidogyne floridensis and two populations of Meloidogyne arenaria

Abstract Cultivar Flordaguard is suggested as a root-knot nematode (RKN) resistant rootstock for Florida peaches, however, RKN disease has been observed on this rootstock in peach orchards. Our goal was to confirm whether the RKN resistance breaking isolates of M. floridensis and M. arenaria indeed could infect and reproduce on the peach rootstock cv. Flordaguard in both laboratory and field studies. Root galling occurred on all peach cultivars evaluated including Flordaguard, Flordaglo, Okinawa, and Lovell, in the presence of the RKN resistance-breaking isolates of M. floridensis (MfGnv14) and two M. arenaria isolates (Ma1 and Ma2). These rootstocks showed varying degrees of susceptibility (to a lesser extent in Okinawa) to these three RKN resistance-breaking isolates. The importance of nematode inoculum concentrations in differentiating between resistance and susceptible plants was demonstrated, and thus are an important factor to consider in nematode resistance breeding programs. In host differential tests the peach-originated isolates of M. floridensis and M. arenaria behaved similarly with the vegetable-originated isolates of M. floridensis on tomato, peanut, watermelon, and tobacco, but showed variable host responses on cotton and pepper. The two M. arenaria isolates from peach reproduced on pepper but not on peanut. To our knowledge this is the first report of M. arenaria race 3 infecting Flordaguard and pepper in Florida. Soil and root samples collected from cv. Flordaguard infected trees at two commercial peach orchards showed that M. floridensis and M. arenaria were established on the rootstock.

Florida growers are considering peach (Prunus persica [L.] Batsch) (Sharpe, 1967) as a viable alternative crop because of the availability of several quality low-chill peach cultivars well-adapted to the subtropical climate and the unique advantage of an early-season market (Olmstead et al., 2015). Peach rootstocks with root-knot nematode (RKN) resistance, a necessary component for the productivity and longevity of an orchard, have primarily been developed in rootstock breeding programs. The peach rootstock cvs. Nemaguard (Prunus persica x P. davidiana) and Nemared (P. persica x P. davidiana), developed by the U.S. Department of Agriculture, and Okinawa, introduced as seed from Japan, were reported resistant to M. incognita, M. javanica, and M. arenaria (Sharpe, 1957;Okie et al., 1985;Esmenjaud et al., 1997). Okinawa and Nemaguard were planted extensively in Florida during the 1960's because they were considered to have a high degree of resistance to the prevalent RKN species M. incognita and M. javanica (Sherman and Lyrene, 1983). These rootstocks, including Nemared, were eventually found to be susceptible to a RKN population in North Florida. Initially the nematode was misidentified as M. incognita race 3 (Sharpe, 1967;Sherman and Lyrene, 1983), however, further investigations led to it being identified as a new species, M. floridensis, common name, the peach RKN (Handoo et al., 2004).
Since its introduction in 1991, the peach seedling rootstock cv. Flordaguard was reported as resistance to all Meloidogyne spp. considered a threat to the peach industry in Florida and was suggested to become the standard rootstock for commercial low-chill peach production replacing Okinawa and Nemaguard (Sherman et al., 1991). After Flordaguard was released as the suggested RKN resistant rootstock, there were reports of it being damaged by Meloidogyne spp. (Brito et al., , 2016. Although limited information is available, recent field surveys carried out in commercial orchards showed that the rootstock was infected by M. floridensis and M. arenaria (Brito et al., 2016). Because Flordaguard is a seedling-propagated rootstock there was speculation that outcrossing resulted in loss of resistance (Nyczepir et al., 2006). Certainly, that could have happened in some orchards but in two commercial orchards where trees were infected by M. floridensis and M. arenaria numerous trees had the characteristic red-leaf stems growing off the rootstock, which suggested the rootstock was true to variety. Furthermore, an isolate of M. floridensis, MFGnv14, was reported inducing severe root galling and reproducing on Flordaguard (Maquilan et al., 2018a). Most recently M. floridensis also was reported causing decline on RKN resistant peach-almond hybrids, Hansen 536 and Bridgt's Hybrid ® 5 rootstocks in California almond orchards (Westphal et al., 2019), and peach rootstock cv. Guardian in South Carolina (Reighard et al., 2019).
Meloidogyne arenaria was also found infecting Flordaguard peach trees in established orchards in Florida. The nematode was causing severe root galling, plant dieback, and stunted growth (Brito et al., 2016;Dickson and Chaparro, pers. comm.). Polyacrylamide gel electrophoresis analyses revealed these M. arenaria populations having an esterase and malate dehydrogenase phenotype identified as EST = A2 and MDH = N3, respectively. These phenotypes differ from the more commonly encountered M. arenaria race 1 phenotype EST = A2; MDH = N1. Phylogenetic analysis performed on the peach infecting M. arenaria populations assigned them as H3 phenotype (Brito et al., 2016).
Our objectives were to: (i) determine infectivity and reproduction on RKN resistant and susceptible peach rootstock cultivars at different inoculum levels of a resistance-breaking M. floridensis isolate; (ii) determine the infectivity of M. floridensis and M. arenaria isolates to three peach rootstock cultivars; (iii) confirm the susceptibility of the resistant Flordaguard rootstock to the resistance-breaking nematode isolate, M. floridensis, and the two M. arenaria isolates; iv) conduct differential host tests of four M. floridensis and three M. arenaria isolates; and v) confirm infection of Flordaguard rootstock by M. floridensis and M. arenaria populations in two commercial peach orchards.

Nematode isolates
Isolates of M. floridensis and M. arenaria used in this study originated from various host crops in several Florida counties (Table 1). Three isolates, Mf1, Mf2, and Mf4 were acquired from the nematode collection of the Division of Plant Industry, Florida Department of Agriculture and Consumer Services, Gainesville, and were characterized using both biochemical and DNA analyses (Brito et al., 2008;. The M. floridensis isolate, Mf4, originated from peach Nemaguard, University of Florida (UF) stone fruit breeding orchard, Gainesville, FL, and is the topotype described by Handoo et al. (2004). Isolate Mf6 originated from a peach orchard located near Ft. Pierce, St. Lucia Co., FL. Another M. floridensis isolate (MfGnv14) originated from a severely galled cv. Flordaguard rootstock found growing in the UF stone fruit orchard. This isolate was used subsequently for genetic studies of resistance in peach rootstocks (Maquilan et al., 2018a(Maquilan et al., , 2018b, as well as comparative genomics of RKN (Szitenberg et al., 2017). The MfGnv14 inoculum used for the current study was collected after two cycles (6 months) of propagation on tomato, whereas the other M. floridensis isolates were maintained over 10 years on greenhouse grown tomato. M. arenaria Ma1 and Ma2 were isolated from commercial peach orchards in Florida in 2014. The M. arenaria (Ma3 isolate) originated from Flordaguard rootstock grown at a commercial orchard located near Auburndale, Polk Co., FL. Each isolate was reared from a single egg mass and cultured on tomato (Solanum lycopersicum L. cv. Agriset 334) at 21°C ± 10°C in the greenhouse.
For all experiments nematode eggs and juveniles were extracted from infected tomato roots using 0.25% NaOCl (Hussey and Barker, 1973), as modified (Boneti and Ferraz, 1981). The nematode suspensions were used immediately to inoculate peach seedlings. In all peach experiments, tomato cv. Agriset 334 was included as a control to monitor inoculum viability. All plants in experiments were watered daily and fertilized weekly (4.5 g/liter of 24N-8P-16K Miracle Gro; Scotts Miracle-Gro Products) (Marysville, OH).

Resistant-breaking M. floridensis isolate on peach rootstocks at three inoculum levels
The infectivity and reproduction of the resistance breaking M. floridensis isolate were determined. The MfGnv14 isolate was applied at 2,000, 5,000, and 10,000 eggs on Flordaguard and Okinawa (both RKN resistant), and a RKN susceptible peach scion cv. Flordaglo. Flordaglo was included to ensure the infectivity of the nematode inoculum on peach.
Cuttings, ca. 15 cm long, of each of the three peach cultivars were prepared for rooting. The cuttings were placed in growing trays containing steam-pasteurized 3:1 mixture of peat-based soil mix (Fafard 2B; Sun Gro Horticulture) (Agawam, MA) and perlite, and allowed to root under a mist propagation system in the greenhouse. After 3 months under the mist system, the rooted cuttings were transplanted into 16-cmdiam. clay pots filled with steam-pasteurized peat and kept in the greenhouse with regulated air temperatures (21 ± 8°C) over the winter. After 6 months (during spring), rooted cuttings were transplanted into 25-cm-diam. clay pots filled with 3:1 mixture of steam-pasteurized sand and perlite. After 1 month, the nematode inoculum was applied into four 3-cmdeep punched holes around the stem base and then pinched closed. The two-factor (inoculum densities and cultivars) factorial designed experiment with four replicates was placed on greenhouse benches in a randomized complete block design. The experiment was terminated 60 days after nematode inoculation.  M. arenaria isolates Ma1, Ma2 on two RKN resistant peach rootstocks Flordaguard, Okinawa, and the RKN susceptible cv. Lovell. Lovell was included to verify the infectivity of the nematode isolates on peach. All seedlings were produced from seed (Blaker et al., 2013, Souza et al., 2017. After 60 to 90 days, germinated seeds were sown in growing trays filled with steampasteurized peat-based soil mix as mentioned above. Seedlings (ca. 20-cm tall) were transplanted into clay pots containing 2:1:1 steam-pasteurized mixture of sand, coarse vermiculite, and perlite. One month later, 4-month-old seedlings were inoculated with 10,000 eggs/plant and maintained for 5 months. The twofactor (isolate and cultivar) experiment was set up on greenhouse benches in a randomized complete block design with seven replicates.

Confirmation of Flordaguard rootstock susceptibility to the RKN resistance breaking isolates
The susceptibility of Flordaguard (RKN resistant rootstock) to M. floridensis isolate MfGnv14 and two M. arenaria isolates Ma1 and Ma2 was tested using 20,000 eggs of each nematode isolate per plant.
One-year-old Flordaguard rooted cuttings were provided by Island Grove Ag Products, Hawthorne, FL. The seedlings were transplanted into pots filled with 1:4 mixture of sand and peat. The trees from which cutting were obtained were from own-rooted Flordaguard trees grown at the UF Plant Science Research and Education Unit, Citra, FL and had been verified as true to variety based on SSR-marker-based fingerprinting (Maquilan et al., 2018b). The experiment consisted of only a single factor (isolate) with eight replicate plants for each of the three isolates.

Differential host tests
The race designation of RKN species and isolates (Table 1) was determined by host differentials. The differentials included: tobacco (Nicotiana tabacum cv. NC95), cotton (Gossypium hirsutum cv. Deltapine 16), pepper (Capsicum annuum cv. California Wonder), watermelon (Citrullus lanatus cv. Charleston Grey), peanut (Arachis hypogaea cv. FL 07), and tomato (cv. Agriset 334). The previously described procedure (Taylor and Sasser, 1978) was modified as follows: freshly hatched and active J2 instead of eggs were used as inoculum to ensure optimal viability. Use of J2 inoculum as an optimum infective stage may resolve conflicting results from previous host-range tests with M. floridensis (Handoo et al., 2004;Kokalis-Burelle and Nyczepir, 2004;Stanley et al., 2009) that might be attributed to the variation in the infection rate (or ability) of J2 when using eggs as starting inoculum. The J2 inoculum was applied to three punched holes (3-cm deep) around the stem base to achieve a concentration of 3,000 J2/plant. Each isolate was inoculated onto the six differential hosts and replicated three to five times in a completely randomized design on greenhouse benches. The test was repeated. Sixty days after inoculation, root systems were removed from the pots, washed, and rated for root galling and egg masses on a 0 to 5 scale (Taylor and Sasser, 1978). Before rating, root systems were soaked in 20% (v/v) red food coloring solution (Chef's Quality; RD/Jet) (College Point, NY) for 20 min to stain egg masses (Thies et al., 2002). Additionally, because no clear root symptoms and (or) nematode sign were observed on the root systems of both cotton and pepper, eggs were then extracted with 1% NaOCl and the final number of eggs (Pf) per gram of fresh roots was counted for each plant and the reproductive factor (RF = Pf/Pi) determined. Host suitability was designated as follows: RF ≥ 1 = good host; 0.1 < RF < 1.0 = poor host; RF ≤ 0.1 = nonhost (Sasser et al., 1984).

RKN infection of peach in commercial peach orchards
Two commercial peach orchards discovered to have Flordaguard rootstock infected by RKNs were designated for soil and root sampling, totaling seven sampling collections between April 2015 to July 2016. Both were stated to have been transplanted with peach scions grafted on Flordaguard rootstock. Orchard A was in central Florida near Auburndale, Polk Co.; orchard B was in south Florida near Fort Pierce, St. Lucie Co. Soil at orchard A, classified as Candler sand with a depth well below 1.5 m, whereas soil at orchard B, classified as an Ankona sand with a depth of ca. 0.05 m, and was stratified over a perched water table. Trees at orchards A and B were ca. 5-and 15-year old, respectively. Soil analyses was conducted by the UF Analytical Services Laboratory, Gainesville, FL (data not shown). Orchard A soil was slightly acidic with a pH 6.0 to 6.4, whereas orchard B soil was strongly acidic with a pH 4.3 to 5.5. The total percentage of silt and clay were less than 3% in the soil samples.
At each site and sampling date, four root-knotnematode-infected trees were arbitrarily selected for collection of root and soil samples based on above ground symptoms that included small tree size, leaf drop, sparse foliage, and crown dieback. Before collecting samples from the top 15 cm of soil and weeds were removed from each sampling site. Samples were collected from the top 50 cm deep layer of soil at three locations around each tree. One liter of soil mixed with peach roots was collected from base of each tree, mixed well and placed in a plastic bag before storing at 10°C until processed (Barker and Nusbaum, 1968). RKN females from galled peach roots were hand-picked and subjected to PAGE for species identification based on esterase (EST) and malate dehydrogenase (MDH) enzymatic profiles (Dickson et al., 1971;Esbenshade and Triantaphyllou, 1985), and DNA analysis when needed (Brito et al., 2016). Nematodes were extracted from 200 cm 3 of soil by centrifugal-flotation method (Jenkins, 1964). Root samples were weighed, and nematodes extracted as stated above. The numbers of nematodes extracted from the soil as well as J2 and eggs from roots were counted. Also, other plantparasitic nematodes ex tracted from soil were identified based on their morphology.

Data analyses
Data were subjected to two-way analysis of variance (ANOVA) from peach experiments 1 (concentration × cultivar) and 2 (isolate × cultivar), and one-way ANOVA for peach experiment 3 (isolate) using R version 3.3.0 (R-Core Team, 2014) with RStudio version 0.99.903 (RStudio, 2014). Variables subjected to analyses were gall index (GI), egg mass index (EMI), reproductive factor (RF), and eggs per gram fresh roots (EGFR). Data were transformed before statistical analyses as follows: GI and EMI by log 10 (x + 1), RF by fourth root, and EGR by arcsine. Means were separated using Tukey's honest significant difference (P ≤ 0.05). Unless otherwise noted, all differences were considered statistically significant at the 0.05 probability level.

Results
Resistance-breaking M. floridenis isolate on peach rootstocks using three inoculum levels Meloidogyne floridensis isolate MfGnv14 induced galls and reproduced on RKN resistant rootstocks Flordaguard, Okinawa, and the susceptible Flordaglo. The gall and egg mass indices were less on Okinawa than on Flordaguard and Flordaglo (Table 2). There were less galling and egg masses induced from the low inoculum of 2,000 eggs/plant, whereas there were no differences between the higher inoculum densities of 5,000 and 10,000 eggs. The level of inoculum and rootstock cultivar both affected the degree of infection and reproduction.

Infectivity of M. floridensis and M. arenaria isolates on three peach rootstocks
Among the three peach rootstocks, only the RKN susceptible Lovell was infected by all three M. floridensis isolates (Mf1, Mf2, Mf4) (Table 3). These three isolates failed to induce galls and did not reproduce on the RKN resistant Flordaguard, in contrast to the heavy infection induced by the RKN resistance breaking isolate MfGnv14 ( Table 2). The three M. floridensis isolates also did not produce galls and failed to reproduce on Okinawa (Table 3), whereas the M. arenaria isolates Ma1 and Ma2 induced galls and egg masses on Flordaguard. On Flordaguard Ma2 induced a higher number of galls and egg masses to a greater extent than Ma1, but this did not correspond to greater egg numbers (Table 3). Only Ma1 was tested on Okinawa, and Lovell because of insufficient inoculum at the time when the plant materials were ready. This isolate produced galls on Okinawa but failed to produce egg masses (Table 3). All isolates infected and reproduced similarly on Lovell.

Confirmation of Flordaguard rootstock susceptibility to the RKN resistance breaking isolates
Meloidogyne floridensis isolate MfGnv14, and two M. arenaria isolates Ma1, Ma2 infected and reproduced on Flordaguard rootstock. There were no differences in galling, egg mass indices, or RF among these three isolates (Table 4).

Differential host tests
All M. floridensis and M. arenaria isolates infected, induced root galling, and reproduced on tomato, watermelon, and tobacco (GI, EMI = 5), but not on peanut ( Table 5). The galls they caused, if any, on cotton and pepper roots were only detectable with aid of a magnifying lamp, however both species reproduced and developed egg masses containing eggs on pepper but not on cotton. Based on the reproductive factor pepper was a better host to M. arenaria isolates than to M. floridensis.

RKN infection of peach in commercial orchards
Meloidogyne arenaria (EST = A3, MDH = N3; Ma phenotype II) was found infecting Flordaguard in orchard A, and M. floridensis (EST = MF3, MDH = N1 phenotype) was found infecting Flordaguard in orchard B. These finding are consistent with identifications of RKN found in Florida (Brito et al., 2008(Brito et al., , 2016. Second-stage juveniles and eggs from egg masses of both species were consistently extracted from soil and roots during a 7-month period at both sites (data not shown). The average number of J2 extracted per 200 cm 3 soil at orchard A and B was 32 and 11, respectively, and eggs per gram of roots averaged 682 and 522 at orchards A and B, respectively. Other plant-parasitic nematodes species were found in soil collected from the peach rhizosphere. The following species were identified: Orchard A -Mesocriconema ornatum and Pratylenchus hippeastri, Orchard B -M. xenoplax and P. brachyurus (Inserra, pers. comm.). Although these nematodes were found in the soil, it was not established that they parasitized peach roots.

Discussion
Sixty days from inoculation of M. floridensis MfGnv14 isolate on peach rootstock showed higher levels of infection on Flordaguard and Flordaglo than on Okinawa. Galling and egg mass indices were higher with inoculum levels of 5,000 and 10,000 compared with 2,000 eggs. Differences in susceptibility were more pronounced at increasing nematode concentrations. At the level of 10,000 eggs/plant, higher EGFR and RF were recorded on Flordaguard and Flordaglo, compared with Okinawa. A different trend was observed at the level of 5,000, wherein Flordaglo supported higher nematode reproduction than Flordaguard, and Okinawa. Differences were not observed at a level of 2,000. Previous reports have suggested that nematode infection increases with increases in inoculum densities (Di Vito et al., 2005;. In an earlier study GI was reported to be well-correlated with RF and the resistance threshold was set to GI = 2 (Maquilan et al., 2018a); thus, because the mean GI value is greater than 2 for all three peach cultivars, they would be classified as susceptible to MfGnv14, although Flordaguard and Flordaglo appeared to be better hosts than Okinawa. These results confirm earlier reports about the lack of resistance to some populations of M. floridensis Notes: a Galling (GI) and egg mass indices (EMI): 0 = no galls or egg masses, 1 = 1-2, 2 = 3-10, 3 = 11-30, 4 = 31-100, 5 = ≥ 100 galls or egg masses per plant (Taylor and Sasser, 1978). GI and EMI data were subjected to log 10 (x + 1) transformation before analysis of variance. Means are average of duplicate tests. Data are non-transformed means of four replicates. Means within a main effect in the same column followed by the same letter are not different (P < 0.05) based on Tukey's honest-significant difference test. b Plant materials were 10-month-old rooted stem cuttings. Tomato cv. Agriset 334 was included to check nematode viability. ns = No statistically significant differences among means.
in Flordaguard (Maquilan et al., 2018a), Okinawa (Sharpe et al., 1969), and Flordaglo (Chaparro, per. comm.). It is noteworthy that at a lower inoculum level (2,000 eggs), Okinawa had a GI = 1.5 and would have been (incorrectly) classified as resistant. It has been demonstrated that, for ligneous Prunus spp., a high and durable inoculum pressure (5,000 to 17,000 J2) would be necessary to obtain clear separation between hosts and nonhosts based on gall indices (Esmenjaud et al., 1992). Our findings suggest that a minimum of 5,000 eggs as inoculum would provide an acceptable level of accuracy after 2 months if galling or egg mass indices are used as a selection criterion.
Among the three peach rootstocks that were tested separately, only Lovell was susceptible to all M. floridensis isolates Mf1, Mf2, Mf4. Lovell supported high levels of nematode reproduction, consistent with previous findings (Stanley et al., 2009   Notes: a Four month-old peach seedlings were inoculated with 10,000 eggs/plant. Peach rootstock cv. Lovell used as a susceptible control and tomato cv. Agriset 334 included to check nematode viability. b Galling (GI) and egg mass indices (EMI) were based on a 0 to 5 scale where 0 = no galls or egg masses, 1 = 1-2, 2 = 3-10, 3 = 11-30, 4 = 31-100, 5 = ≥ 100 galls or egg masses per plant (Taylor and Sasser, 1978). Data were subjected to log 10 (x + 1) transformation before analysis of variance. Data are non-transformed means of seven replicates. c EGFR = Eggs per gram of fresh root. Reproduction factor (RF) = ratio of nematode eggs at 5 months after inoculation to initial inoculum concentration of 10,000 eggs (Sasser et al., 1984). EGFR and RF data were subjected to arcsine and fourth-root transformation, respectively, before analysis of variance. Data are non-transformed means of seven replicates.
from Nemaguard peach roots. Previously, this isolate was able to induce only 10 to 20% root galling on Flordaguard (compared to 80 to 100% in Guardian and Nemaguard peach rootstocks) after 1 year in microplots, which became the basis for considering Flordaguard as resistant to M. floridensis (Nyczepir et al., 2006). Several other Prunus species including some P. cerasifera accessions such as P.2980, P.2175, and P.1079 showed complete resistance (GI = 0) to the M. floridensis topotype (Esmenjaud et al., , 2009Lecouls et al., 1997;Rubio-Cabetas et al., 1998). In our experiment, the MfGnv14 isolate induced heavy infection on Flordaguard, which confirms previous results indicating that MfGnv14 isolate is a resistancebreaking variant of M. floridensis (Maquilan et al., 2018a). Both Ma isolates induced galls and reproduced on Flordaguard, although Ma1 produced fewer galls and egg masses than Ma2. Ma1 caused galls on Okinawa, but no egg masses or eggs were recovered. Lovell supported a higher level of nematode galling and reproduction compared to Flordaguard. Okinawa appeared to be a poor host for M. arenaria, which might be due to a slower rate of development of juveniles in the roots or the inability of the nematode to complete its life cycle in Okinawa-a similar phenomenon was observed on Guardian when infected with M. incognita juveniles (Nyczepir et al., 1999). However, if galling intensity is used as a sole criterion for classifying resistance, a galling index that is greater than the resistance threshold (GI > 2) may not be practically significant for breeding when a source of high resistance (GI = 0) is needed in the development of new rootstocks (Maquilan et al., 2018a).
Peach rootstock Flordaguard was susceptible to the M. floridensis isolate MfGnv14 and both M. arenaria isolates, providing confirmatory evidence that these isolates overcome the RKN resistance in Flordaguard. Given that M. floridensis is a facultative meiotic parthenogenetic species capable of sexual reproduction under conditions of stress and crowding, genetic variation arising from cross-fertilization would facilitate an adaptive response to host-plant resistance (Handoo et al., 2004;Castagnone-Serena, 2006). When nematode resistance genes in RKN resistant rootstocks become nonfunctional it is imperative to seek new sources of resistance and understand the underlying mechanism of resistance against these nematode species to enhance breeding strategies.
Based on host differential tests, M. floridensis isolates Mf1, Mf2, Mf6 produced less marked rootgalling on cotton, but failed to reproduce, whereas isolate MfGnv14, and M. arenaria isolates Ma1 and Ma3 did not produce galls nor reproduce on cotton. Based on the EMI as a reproductive parameter, tomato, watermelon, and tobacco were susceptible to M. floridensis and M. arenaria, whereas cotton and peanut were nonhosts. These findings with M. floridensis are consistent with those of a previous study (Stanley  Notes: a Galling (GI) and egg mass (EMI) indices were based on a 0 to 5 scale where 0 = no galls or egg masses, 1 = 1-2, 2 = 3-10, 3 = 11-30, 4 = 31-100, 5 = >100 galls or egg masses per plant (Taylor and Sasser, 1978). Data were subjected to log 10 (x + 1) transformation before analysis of variance. Data are non-transformed means of eight replicates. b EGFR = Eggs per gram of fresh root weight. Reproduction factor (RF) = ratio of nematode eggs at 5 months after inoculation to initial inoculum concentration of 20,000 eggs (Sasser et al., 1984). EGFR and RF data were subjected to arcsine and fourth-root transformation, respectively, before analysis of variance. Data are non-transformed means of eight replicates. Means in the same column followed by the same letter are not different (P < 0.05) based on Tukey's honest-significant difference test. ns = No statistically significant differences among means.  Galling (GI) and egg mass (EMI) indices were based on a 0 to 5 scale, where 0 = no galls or egg masses, 1 = 1-2, 2 = 3-10, 3 = 11-30, 4 = 31-100, 5 = ≥100 galls or egg masses per plant (Taylor and Sasser, 1978). c Reproduction factor (RF) = ratio of nematode eggs at 60 days post inoculation to 3,000 second-stage juveniles initially used as inoculum (Sasser et al., 1984 (Handoo et al., 2004). M. floridensis produced pronounced root-galling on tobacco at inoculum levels of 3,000 freshly hatched J2 compared to an inoculum level of 5,000 eggs (Stanley et al., 2009) or 2,000 eggs (Handoo et al., 2004). Variability in host suitability outcomes after 60 days may be affected by differences in inoculum type, concentrations, and greenhouse conditions. It appears to be important to consider nematode developmental stage and concentration when choosing inoculum for an efficient screening method. On pepper, M. floridensis and M. arenaria isolates exhibited variability in their reproductive capabilities. The four M. floridensis isolates produced a low reproductive factor, whereas the three M. arenaria isolates produced a higher reproductive factor. The M. floridensis and M. arenaria isolates produced no readily visible galls on pepper, however, isolates Ma2 and Ma3 caused a trace of small galls. Based on these results cotton would be considered a nonhost (0.1 < RF <1.0), whereas pepper would be considered a good host to M. arenaria (RF ≥ 1). Previous reports have been inconsistent regarding host suitability of pepper to M. floridensis either being reported as a good host (Stanley et al., 2009) or as a nonhost (Kokalis-Burelle and Nyczepir, 2004). Consistent with the latter study's finding, the lower values for EMI and RF from the four M. floridensis isolates in the present study suggest that pepper cv. California Wonder is a poor host. In a growth chamber experiment at 28°C, M. floridensis was found incapable of infecting and reproducing on California Wonder 30 days after inoculation with 240 J2 (Maquilan et al., 2020). Of the six differential hosts, variations in reproductive potential among M. floridensis and M. arenaria isolates were observed only on pepper; therefore, additional hosts including major plant cultivars would be necessary to demonstrate greater differences among these isolates.
Three host races have been reported for M. arenaria in Spain (Fargette, 1987;Robertson et al., 2006). The three M. arenaria Florida isolates from peach reproduced on pepper but not on peanut, corresponding to the host range defined for race 3. This is the first report of the occurrence of M. arenaria race 3 in the United States. In addition to finding M. arenaria race 3 in Spain, the race has also been reported in Uruguay (Fargette, 1987;Robertson et al., 2006Robertson et al., , 2009Devran and Söğüt, 2011).
In two commercial peach orchards designated in this study, orchard A was infested with M. arenaria, and orchard B was infested with M. floridensis. RKN galls and egg masses were consistently recovered from peach roots sampled during the 7-month period, establishing that Flordaguard served as a host of this nematode species. There is little information regarding M. arenaria infecting and reproducing on peach in Florida, however, this pathogen is frequently encountered infecting other crops throughout the state.
In summary, the peach cv. Flordaguard was infected by M. floridensis (MfGnv14) and M. arenaria (Ma1 and Ma2). This was confirmed by testing of MfGnv14 recovered from the UF stone fruit breeding orchard and the two M. arenaria isolates on true to variety Flordaguard seedlings in greenhouse tests. In addition, both nematode species were found infecting the rootstock in commercial field plantings. These virulent isolates would be useful for future genetic studies to identify new sources of RKN resistance as well as to investigate the allelic relationships of RKN resistance loci such as Mf (conferring resistance to M. floridensis) in Prunus kansuesis (Maquilan et al., 2018a(Maquilan et al., , 2018b and RMia (conferring resistance to M. arenaria as well as M. incognita), which may be present in Okinawa (Duval et al., 2014). These would be critical research directions for future developments of improved rootstocks. The peach-originated isolates of M. floridensis and M. arenaria behaved variably on cotton and pepper with less marked root-galling on cotton and pepper, respectively.