A LU:−16 individual with antibodies


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American National Red Cross

Subject: Medical Laboratory Technology


ISSN: 0894-203X
eISSN: 1930-3955





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VOLUME 33 , ISSUE 3 (September 2017) > List of articles

A LU:−16 individual with antibodies

Carole Éthier / Cynthia Parent / Anne-Sophie Lemay / Nadia Baillargeon / Geneviève Laflamme / Josée Lavoie / Josée Perreault / Maryse St-Louis *

Keywords : Lutheran, high-prevalence antigen, African, pregnancy

Citation Information : Immunohematology. Volume 33, Issue 3, Pages 110-113, DOI: https://doi.org/10.21307/immunohematology-2019-016

License : (Transfer of Copyright)

Published Online: 09-October-2019



Antibodies against Lutheran blood group antigens have been observed during first-time pregnancy. Samples from a woman of African descent were tested in our immunohematology laboratory on several occasions since 2001. Her samples were phenotyped as Lu(a+b−), and anti-Lub was suspected but not identified. She was asked to make autologous donations in preparation for her delivery, which she did. In 2010, two antibodies were identified: anti-Lea and -Lub. Six years later, a third investigation was requested. This time, an antibody directed at a high-prevalence Lutheran antigen was found in addition to the anti-Lea and -Lub previously observed. Her serum was compatible with three out of five Lu(a−b−) reagent red blood cells (RBCs). One of the incompatible Lu(a−b−) reagent RBCs was known to be In(Lu) (KLF1 mutation). The genetic background of the other reagent RBC was unknown. The LU cDNA sequence analysis revealed the presence of the c.230G>A (Lua), c.679C>T (LU:–16), and a silent polymorphism c.1227G>T. Anti-Lu16 was highly suspected. This would be the fifth case of LU:–16 with antibodies reported, all within women of African heritage with the Lu(a+b−) phenotype. Hemolytic disease of the fetus and newborn was not noted in these cases.

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1. ISBT Web site: http://www.isbtweb.org, accessed April 5, 2017.

2. Karamatic Crew V, Laundy R, Bahashwan A, et al. Two novel high incidence antigens in the Lutheran blood group system (LUAC and LUBI). Vox Sang 2016;111(Suppl 1):63.

3. Reid ME, Lomas-Francis C, Olsson ML. The blood group antigen factsbook. 3rd ed. London: Academic Press, 2012: 263–95.

4. El Nemer W, Rahuel C, Colin Y, et al. Organization of the human LU gene and molecular basis of the Lua/Lub blood group polymorphism. Blood 1997;89:4608–16.

5. Parsons SF, Mallinson G, Daniels GL, et al. Use of domaindeletion mutants to locate Lutheran blood group antigens to each of the five immunoglobulin superfamily domains of the Lutheran glycoprotein: elucidation of the molecular basis of the Lua/Lub and the Aua/Aub polymorphisms. Blood 1997;89:4219–25.

6. Karamatic Crew V, Green C, Daniels G. Molecular bases of the antigens of the Lutheran blood group system. Transfusion 2003;43:1729–37.

7. Karamatic Crew V, Poole J, Banks J, et al. LU21: a new highfrequency antigen in the Lutheran blood group system. Vox Sang 2004;87:109–13.

8. Karamatic Crew V, Thornton N, Burton N, et al. Two heterozygous mutations in an individual result in the loss of a novel high incidence Lutheran antigen Lurc. Tranfus Med 2009;19(Suppl 1):10.

9. Hustinx H, Lejon-Crottet S, Henny C, et al. LUIT: a novel high incidence antigen in the Lutheran blood group system. Vox Sang 2014;107(Suppl 1):172.

10. Brennan S, Shakarian G, Vege S et al. A new antibody in the Lutheran blood group system against a novel high-frequency antigen named LUGA. Transfusion 2015;55(Suppl 3):36A.

11. Sabo B, Pancoska C, Myers M, et al. Antibodies against two high frequency antigens of the Lutheran system. Lu:2 and Lu:16, made by Lu(a+b−) black females. Transfusion 1980;20:630.

12. Denomme GA, Fueger JT, Lomas-Francis C, et al. An example of anti-Lub and anti-Lu16 identified early in the gestation of an initial pregnancy. Transfusion 2010;50:155A.

13. Storry JR, Olsson ML, Moulds JJ. Rabbit red blood cell stroma bind immunoglobulin M antibodies regardless of blood groups specificity. Transfusion 2006;46:1260–1.

14. Erythrogene Web site: http://www.erythrogene.com, accessed February 22, 2017. 

15. SCARF: http://scarfex.jove.prohosting.com, accessed April 5, 2017.