American National Red Cross
Subject: Medical Laboratory Technology
ISSN: 0894-203X
eISSN: 1930-3955
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T. Horn * / J. Hamilton / J. Kosanke / V.W. Hare / W. Kluver / W. Beres / S. Nance / M.A. Keller
Keywords : antigen phenotype, microhematocrit separation, EDTA glycine acid (EGA), hypotonic saline wash, RBC genotyping
Citation Information : Immunohematology. Volume 33, Issue 4, Pages 147-151, DOI: https://doi.org/10.21307/immunohematology-2019-020
License : (CC-BY-NC-ND 4.0)
Published Online: 16-October-2019
For patients requiring multiple transfusions and patients with positive direct antiglobulin tests (DATs), an extended red blood cell (RBC) phenotype can provide valuable information and help to determine the risk of forming alloantibodies. In some instances, the phenotype may be used for prophylactic matching. Phenotyping in this patient population is often hindered by the presence of circulating donor cells and/or by a positive DAT. Several methods, such as EDTA glycine acid (EGA) treatment to remove IgG, hypotonic saline wash to separate autologous RBCs, or reticulocyte separation, are often used in these situations to isolate patient RBCs for serologic phenotyping. This study aimed to determine the accuracy of each RBC pretreatment method by comparing serologically determined antigen types with those predicted by RBC genotyping. Forty-eight peripheral blood samples from recently transfused patients were phenotyped for selected antigens in the Rh, Kell, MNS, Duffy, and Kidd systems. Treatment methods for the sample sets were reticulocyte separation (