Proposed criterion for distinguishing ABO mosaics from ABO chimeras using flow cytometric analysis

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Immunohematology

American National Red Cross

Subject: Medical Laboratory Technology

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ISSN: 0894-203X
eISSN: 1930-3955

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VOLUME 31 , ISSUE 1 (March 2015) > List of articles

Proposed criterion for distinguishing ABO mosaics from ABO chimeras using flow cytometric analysis

Akira Oda / Nobuki Matsuyama / Mizuko Hirashima / Hiroyuki Ishii / Keiko Kimura / Harumichi Matsukura / Fumiya Hirayama / Keisei Kawa / Yasuo Fukumori

Keywords : ABO, mosaic, chimera, flow cytometry  

Citation Information : Immunohematology. Volume 31, Issue 1, Pages 24-28, DOI: https://doi.org/10.21307/immunohematology-2019-067

License : (Transfer of Copyright)

Published Online: 26-October-2019

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ABSTRACT

Differentiation of ABO mosaics from chimeras is performed using flow cytometry (FCM) analysis. Although mosaics and chimeras have been distinguished by presence or absence of clear resolution using FCM analysis, the lack of quantitative metrics and definitive criteria for this differentiation has made some cases difficult to differentiate. In this study, therefore, we attempted to establish a definitive and quantitative criterion for this differentiation. When FCM histogram gates for group “A” or “B” antigen-negative and -positive red blood cells (RBCs) were set such that group O RBCs were classified as 99 percent negative and group A or B RBCs as 99 percent positive, the percentages of RBCs in the middle region of six chimeras and 23 mosaics (12 A mosaics and 11 B mosaics) were 0.1–0.6 percent and 7.0–19.0 percent, respectively. This result suggested that ABO mosaics and chimeras can be unambiguously differentiated when the cutoff point of the intermediate region is set to 1 percent.

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