An overview of the use of SNaPshot for predicting blood group antigens

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Immunohematology

American National Red Cross

Subject: Medical Laboratory Technology

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ISSN: 0894-203X
eISSN: 1930-3955

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VOLUME 31 , ISSUE 2 (June 2015) > List of articles

An overview of the use of SNaPshot for predicting blood group antigens

Flavia R.M. Latini / Lilian M. Castilho *

Keywords : SNaPshot, blood group alleles, single nucleotide polymorphisms, multiplex-PCR  

Citation Information : Immunohematology. Volume 31, Issue 2, Pages 53-57, DOI: https://doi.org/10.21307/immunohematology-2019-070

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ABSTRACT

The use of SNaPshot (Applied Biosystems, Foster City, CA) for predicting blood group antigens has emerged as an alternative to hemagglutination testing and also to the current low- and highthroughput blood group genotyping methods. Several groups have developed multiplex–polymerase chain reaction SNaPshot assays to determine single nucleotide polymorphisms (SNPs) in blood group genes with the purpose of identifying clinically relevant antigens and rare alleles. The selection of SNPs is based on the population or laboratory reality and the purpose of the genotyping. Unlike high-throughput genotyping strategies that are provided as commercial platforms, the SNPs can be chosen to best meet the needs of the user, and the interpretation of the results do not depend on the manufacturer.

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