EDTA glycine acid treatment of red blood cells

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Immunohematology

American National Red Cross

Subject: Medical Laboratory Technology

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ISSN: 0894-203X
eISSN: 1930-3955

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VOLUME 28 , ISSUE 3 (September 2012) > List of articles

EDTA glycine acid treatment of red blood cells

Joanne Kosanke

Keywords : EGA, EDTA glycine acid, IgG dissociation, direct antiglobulin test, DAT

Citation Information : Immunohematology. Volume 28, Issue 3, Pages 95-96, DOI: https://doi.org/10.21307/immunohematology-2019-157

License : (Transfer of Copyright)

Published Online: 01-December-2019

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ABSTRACT

IgG dissociation is necessary when a sample is direct antiglobulin test (DAT) positive and antigen testing using blood grouping serum reactive by the antiglobulin test is performed. Exposure of IgG-coated red blood cells (RBCs) to a low pH of 3.0 with EDTA glycine acid successfully dissociates the IgG, rendering the RBCs DAT negative 82 to 85 percent of the time. The procedure takes one minute or less and leaves RBC antigens intact and able to be typed except for those antigens in the Kell blood group system and for the high-prevalence antigen Era.

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