ABSTRACT

Alsever’s solution has been used for decades as a preservative solution for storage of RBCs. From October 2005 to January 2006, unexplained hemagglutination of approximately 10 to 20 percent of RBCs stored for several days in a modified version of Alsever’s solution was noticed in quality control testing at the Canadian Blood Services Serology Laboratory. An investigation,including microbial testing,was initiated to determinethe cause of the unexplainedhemagglutination. The gram-negative bacterium Serratia liquefaciens was isolated from supernatant solutions of agglutinated RBCs. Further characterization of this strain revealed that it has the ability to form biofilms; presents high levels of resistance to chloramphenicol,neomycin,and gentamicin;and causes mannose-sensitive hemagglutination. The source of S.liquefaciens contamination in RBC supernatants was not found. However,this bacterium has not been isolated since January 2006 after enhanced cleaning practices were implemented in the serology laboratory where the RBCs are stored.This biofilm-forming,antibiotic-resistant S.liquefaciens strain could be directly linked to the unexplained hemagglutination observed in stored RBCs.