Reduced red blood cell destruction by antibody fragments

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Immunohematology

American National Red Cross

Subject: Medical Laboratory Technology

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ISSN: 0894-203X
eISSN: 1930-3955

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VOLUME 22 , ISSUE 1 (March 2006) > List of articles

Reduced red blood cell destruction by antibody fragments

Amina Mqadmi / Steven Abramowitz / Xiaoying Zheng / Karina Yazdanbakhsh

Keywords : antibody fragments, F(ab´)2, red blood cells, RBC, survival, destruction, glycophorin A, Ter-119, mice, transfusion

Citation Information : Immunohematology. Volume 22, Issue 1, Pages 11-14, DOI: https://doi.org/10.21307/immunohematology-2019-340

License : (Transfer of Copyright)

Published Online: 01-April-2020

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ABSTRACT

Antibodies to blood group antigens can cause immune RBC destruction directly (extravascular destruction) or indirectly through subsequent complement activation (intravascular hemolysis). The Fc portion of the IgG antibody is responsible for the effector functions of immune RBC destruction. We hypothesized that sensitization of RBCs with blood group antigen–specific IgG antibodies lacking their Fc portion would escape from the recipient’s immune system, allowing for a longer survival period of the RBCs in the circulation. Direct injection of mouse RBC-specific Ter-119 monoclonal antibody into mice resulted in a more severe anemia compared with that in mice injected with the Ter-119 F(ab´)2 fragment. We found that mouse RBCs coated in vitro with the Ter-119 F(ab´)2 fragment, when transfused into mice,survived longer in circulation compared with RBCs coated with whole Ter-119 IgG molecule. The data support the conclusion that antibodies can be rendered less pathogenic through removal of their Fc portion.

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