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Citation Information : Immunohematology. Volume 18, Issue 4, Pages 109-113, DOI: https://doi.org/10.21307/immunohematology-2019-526
License : (Transfer of Copyright)
Published Online: 14-October-2020
In two to five percent of cases of autoimmune hemolytic anemia (AIHA),the patient’s RBCs are negative in the direct antiglobulin test (DAT). We describe a patient with AIHA with a negative DAT when tested by the traditional tube test but with a strongly positive one when tested by column agglutination. When the DAT was repeated by tube test using 4°C saline washes, it became positive. This phenomenon has been observed when low-affinity antibodies are involved in AIHA. The patient’s history and serologic findings are detailed in this report.