Comparison of three low-ionic diluents for dilution and storage of reagent A1 and B cells for testing in gel technology

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Immunohematology

American National Red Cross

Subject: Medical Laboratory Technology

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ISSN: 0894-203X
eISSN: 1930-3955

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VOLUME 17 , ISSUE 2 (June 2001) > List of articles

Comparison of three low-ionic diluents for dilution and storage of reagent A1 and B cells for testing in gel technology

E. Ann Steiner / LouAnn Dake

Keywords : gel technology, ABO serum group, blood typing in gel, MTS Diluent 2 Plus

Citation Information : Immunohematology. Volume 17, Issue 2, Pages 53-56, DOI: https://doi.org/10.21307/immunohematology-2019-545

License : (Transfer of Copyright)

Published Online: 14-October-2020

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ABSTRACT

Currently, ABO serum grouping performed by gel technology employs a red cell diluent containing EDTA (MTS Diluent 2 Plus™) that does not permit extended storage of the red cell suspensions. A diluent currently used for suspension and long-term storage of reagent red cells for antibody detection and identification (Ortho 0.8% Red Cell Diluent™) was evaluated for use with A1 and B cells. Because this diluent does not contain EDTA, testing was limited to EDTA samples. As a comparison, a Micro Typing Systems (MTS) diluent not containing EDTA (MTS Diluent 2™) was also tested. MTS-suspended red cells were maintained for 24 hours and compared with Ortho-Clinical Diagnostics 0.8% suspended red cells maintained for 7 days. ABO serum grouping was performed on 144 EDTA plasma samples using all three cell suspensions. Acceptable results were noted in all aspects of testing.

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