Easy method for determining the frequency of O1 and O2 alleles in Brazilian blood donors by PCR-RFLP analysis

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Immunohematology

American National Red Cross

Subject: Medical Laboratory Technology

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ISSN: 0894-203X
eISSN: 1930-3955

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VOLUME 17 , ISSUE 4 (December 2001) > List of articles

Easy method for determining the frequency of O1 and O2 alleles in Brazilian blood donors by PCR-RFLP analysis

Ana C. Batissoco / Marcia C.Z. Novaretti / Valdecir J. Bueno / Pedro E. Dorlhiac-Llacer / Dalton A.F. Chamone

Keywords : ABO blood group, ABO genotypes, O1 allele, O2 allele, blood donors, PCR, RFLP, blood group frequencies

Citation Information : Immunohematology. Volume 17, Issue 4, Pages 111-116, DOI: https://doi.org/10.21307/immunohematology-2019-563

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Published Online: 14-October-2020

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ABSTRACT

Serologic ABO blood typing is routinely performed using anti-A and anti-B sera to distinguish four phenotypes (A, B, AB, and O). Restriction fragment length polymorphisms (RFLPs) and DNA sequence studies offer the possibility of direct ABO genotyping. We used polymerase chain reaction-RFLP analysis to determine the frequency of O1 and O2 alleles in 82 unrelated blood donors in São Paulo, Brazil, known to be group O. Genomic DNA was extracted from blood leukocytes by a modified salting-out method. Different genotypes (O1O1, O1O2, O2O2) were identified after digestion with restriction enzymes KpnI, HpaII, and AluI, followed by agarose gel electrophoresis. Of 82 samples analyzed, 74 were O1O1, 7 were O1O2 , and 1 was O2O2. These results showed the frequency of O1O1 , O1O2, and O2O2 genotypes to be 90.24 percent, 8.53 percent, and 1.22 percent, respectively, in blood donors in São Paulo, Brazil.

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