Characterization of human anti-hrB-like monoclonal antibody

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Immunohematology

American National Red Cross

Subject: Medical Laboratory Technology

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ISSN: 0894-203X
eISSN: 1930-3955

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VOLUME 12 , ISSUE 3 (September 1996) > List of articles

Characterization of human anti-hrB-like monoclonal antibody

Antoine P. Blancher / Marion E. Reid / Simone J. Alié-Daram / Jean Michel H. Dugoujon / Francis L. Roubinet

Citation Information : Immunohematology. Volume 12, Issue 3, Pages 119-122, DOI: https://doi.org/10.21307/immunohematology-2019-762

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Published Online: 16-November-2020

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ABSTRACT

To develop a blood typing reagent with Rh specificity, peripheral blood lymphocytes were isolated from a multiparous woman with apparent alloanti-Ce and fused with FIS heteromyeloma cells. One hybridoma cell line, FOR-2E3, secreted human IgG, which agglutinated all human red blood cells except R2R2, RZRZ, Dc–, D––, Rhnull, and e+ hrB–. The supernatant is useful as a reagent to screen for hrB– blood donors and to differentiate haplotypes with C and e in cis from haplotypes with C and e in trans.

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