Rhmod phenotype: a parentage problem solved by denaturing gradient gel electrophoresis of genomic DNA

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Immunohematology

American National Red Cross

Subject: Medical Laboratory Technology

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ISSN: 0894-203X
eISSN: 1930-3955

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VOLUME 12 , ISSUE 4 (December 1996) > List of articles

Rhmod phenotype: a parentage problem solved by denaturing gradient gel electrophoresis of genomic DNA

Fiona J. Steers / Maura Wallace / Marialuisa Mora / Ben Carritt / Patricia Tippett / Geoff Daniels

Citation Information : Immunohematology. Volume 12, Issue 4, Pages 154-158, DOI: https://doi.org/10.21307/immunohematology-2019-770

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Published Online: 16-November-2020

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ABSTRACT

Initial Rh phenotyping of a man with hemolytic anemia, his wife, and son appeared to exclude paternity. No exclusion was found in other blood groups or in the human leukocyte antigen (HLA) system; excluding Rh, the paternity index was 98.58 percent. Samples from these three family members, and two other family members, were tested with additional Rh antisera. The results indicated that the propositus has an Rhmod phenotype with expression of c, weak e, and very weak D, E, and G antigens. To support this hypothesis, DNA analysis of the RHD and RHCE genes was performed on the five family members. Polymerase chain reaction (PCR) products from exons 2 and 5 were analyzed by denaturing gradient gel electrophoresis (DGGE). The DNA results corroborated the serologic findings and refuted the exclusion of paternity.

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