SEARCH WITHIN CONTENT
Citation Information : Immunohematology. Volume 11, Issue 3, Pages 71-73, DOI: https://doi.org/10.21307/immunohematology-2019-792
License : (Transfer of Copyright)
Published Online: 17-November-2020
ABO discrepancy was noted when a patient’s unwashed saline red cell suspension was tested with monoclonal ABO reagents. The discrepancy was resolved when a washed (x 3) saline red cell suspension was used in repeat testing. The patient’s serum contained an autoagglutinin that reacted optimally below pH 7.0. The discrepancy occurred when monoclonal ABO reagents, formulated at a low pH, lowered the pH of the reaction mixture, and autoagglutination was observed.