Expression of B and H antigens on red cells from a group Bweak individual studied by serologic and scanning electron microscopic techniques

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Immunohematology

American National Red Cross

Subject: Medical Laboratory Technology

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ISSN: 0894-203X
eISSN: 1930-3955

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VOLUME 8 , ISSUE 4 (December 1992) > List of articles

Expression of B and H antigens on red cells from a group Bweak individual studied by serologic and scanning electron microscopic techniques

Hans Erik Heier / Leif Kornstad / Ellen Namork / Peder Østgard / Randi Sandin

Citation Information : Immunohematology. Volume 8, Issue 4, Pages 94-99, DOI: https://doi.org/10.21307/immunohematology-2019-994

License : (Transfer of Copyright)

Published Online: 06-December-2020

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ABSTRACT

The proposita was classified as Bel, By, or Bm, Le(b+) by routine blood grouping and by adsorption/elution studies using anti-A and -B hyperimmune pregnancy sera. Red cells from the proposita adsorbed as much anti-B from the hyperimmune sera as did red cells from normal B individuals, but adsorbed less anti-A,B (group O serum). Saliva contained H, but not B, soluble substance. Red cells from the proposita and a normal B donor were sensitized with monoclonal A and B blood group antibodies immunolabeled with colloidal gold particles, and examined in a scanning electron microscope. B antigens were found on more than 95 percent of normal B cells, but on only 2-3 percent of red cells from the proposita. However, when the same cells were sensitized with anti-A,B that reacted strongly with B oligosaccharides other than type 2 chains, half of the labeled red cells from the proposita were labeled more strongly than any normal B cells. Our results explain why red cells from the proposita adsorb significant amounts of anti-B and anti-A,B without being agglutinated by these antibodies. The results of both adsorption/elution and immunolabeling suggest that the B antigen on her cells differs biochemically from that on normal B cells.

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