Morphological and Molecular Characterization of Two Aphelenchoides Endophytic in Poplar Leaves


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Journal of Nematology

Society of Nematologists

Subject: Life Sciences


ISSN: 0022-300X
eISSN: 2640-396X





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VOLUME 48 , ISSUE 1 (March 2016) > List of articles

Morphological and Molecular Characterization of Two Aphelenchoides Endophytic in Poplar Leaves


Keywords : nematode ecology, phylogeny, ribosomal DNA, systematics, taxonomy.

Citation Information : Journal of Nematology. Volume 48, Issue 1, Pages 28-33, DOI:

License : (CC BY 4.0)

Received Date : 30-September-2014 / Published Online: 21-July-2017



During a long-term, large network study of the ecology of plant endophytes in native habitats, various nematodes have been
found. Two poplar species, Populus angustifolia (narrowleaf cottonwood) and Populus trichocarpa (black cottonwood), are important ecological and genomic models now used in ongoing plant–pathogen–endophyte interaction studies. In this study, two different aphelenchid nematodes within surface-sterilized healthy leaves of these two Populus spp. in northwestern North America were discovered. Nematodes were identified and characterized microscopically and molecularly with 28S ribosomal RNA (rRNA) and 18S rRNA molecular markers. From P. angustifolia, Aphelenchoides saprophilus was inferred to be closest to another population of A. saprophilus among sequenced taxa in the 18S tree. From P. trichocarpa, Laimaphelenchus heidelbergi had a 28S sequence only 1 bp different from that of a Portuguese population, and 1 bp different from the original Australian type population. The 28S and 18S rRNA trees of Aphelenchoides and Laimaphelenchus species indicated L. heidelbergi failed to cluster with three other Laimaphelenchus species, including the type species of the genus. Therefore, we support a conservative definition of the genus Laimaphelenchus, and consider these populations to belong to  Aphelenchoides, amended as Aphelenchoides heidelbergi n. comb. This is the first report of these nematode species from within aboveground leaves. The presence of these fungal-feeding nematodes can affect the balance of endophytic fungi, which are important determinants of plant health.

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  1. Bajaj, H. K., andWalia, K. K. 2000. A new species of Laimaphelenchus Fuchs, 1937 (Nematoda : Aphelenchina) from Kalesar forest, Haryana, India. Indian Journal of Nematology 30:88–90.
  2. Baynes, M. A., Russell, D. M., Newcombe, G., Carta, L. K., Rossman, A. Y., and Ismaiel, A. 2012. A mutualistic interaction between a fungivorous nematode and a fungus within the endophytic communityof Bromus tectorum. Fungal Ecology 5:610–623.
  3. Busby, P. E., Zimmerman, N.,Weston, D. J., Jawdy, S. S., Houbraken, J., and Newcombe, G. 2013. Leaf endophytes and Populus genotype affect severity of damage from the necrotrophic leaf pathogen, Drepanopeziza populi. Ecosphere 4:125.
  4. Carta, L., Skantar, A. M., Handoo, Z. A., and Baynes, M. A. 2011. Supplemental description of Paraphelenchus acontioides (Tylenchida: Paraphelenchidae), with ribosomal DNA trees and a morphometric compendium of female Paraphelenchus. Nematology 13:887–899.
  5. Franklin, M. T. 1957. Aphelenchoides composticola n. sp. and A. saprophilus n. sp. from mushroom compost and rotting plant tissues. Nematologica 2:306–313.
  6. Golden, A. M. 1990. Preparation and mounting nematodes for microscopic observation. Pp. 197–205 in B. M. Zuckerman, W. F. Mai, and L. R. Krusberg, eds. Plant Nematology Laboratory Manual. Amherst, MA: University of Massachusetts Agricultural Experiment Station.
  7. Hacquard, S., and Schadt, C. W. 2015. Towards a holistic understanding of the beneficial interactions across the Populus microbiome. New Phytologist 205:1424–1430.
  8. Hirling, W. 1982. A survey of the species of the nematode genera Laimaphelenchus Fuchs, 1937 and Ruidosaphelenchus Laumond and Carle 1971, with keys to species and three new species of Laimaphelenchus
    (Nematoda: Tylenchida). Zeitschrift f€ur Pflanzenkrankheiten und Pflanzenschutz 89:30–42.
  9. Hirling,W. 1984/1985, publ. 1986. Laimaphelenchus penardi (Nematoda:Tylenchida) fromthe type locality and contribution on the identity, biology and distribution of this nematode and of three related species, L. montanus,
    L. sylvaticus and L. praepenardi n. sp. Zoologische Beitr€age 29:349–375.
  10. Holterman, M., van derWurff, A., van den Elsen, S., van Megen, H., Bongers, T., Holovachov, O., Bakker, J., and Helder, J. 2006. Phylumwide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown Clades. Molecular Biology and Evolution 23:1792–1800.
  11. Huelsenbeck, J. P., and Ronquist, F. 2001. MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755.
  12. Kanzaki, N., Giblin-Davis, R. M., Scheffrahn, R. H., Center, B. J., and Davies, K. A. 2009. Pseudaphelenchus yukiae n. gen., n. sp. (Tylenchina: Aphelenchoididae) associated with Cylindrotermes macrognathus (Termitidae:Termitinae) in La Selva, Costa Rica. Nematology 11:869–881.
  13. Maleita, C., Costa, S. R., and Abrantes, I. 2014. First report of Laimaphelenchus heidelbergi (Nematoda: Aphelenchoididae) in Europe.Forest Pathology 45:76–81.
  14. Negi, S., and Ye, J.-R. 2011. Occurrence of nematode species associated with pine wood in Jiangsu, Zhejiang andGansu provinces in China. Journal of Nanjing Forestry University (Natural Sciences Edition) 35:149–150.
  15. Nunn, G. 1992. Nematode molecular evolution. An investigation of evolutionary patterns among nematodes based upon DNA sequences. Ph.D. dissertation, University of Nottingham, UK.
  16. Oro, V. 2015. Description of Laimaphelenchus belgradiensis sp. nov. (Nematoda: Aphelenchoididae) and its phylogenetic and systematic position within Aphelenchoidoidea. European Journal of Plant Pathology 142:13–23.
  17. Raghavendra, A. K. H., and Newcombe, G. 2013. The contribution of foliar endophytes to quantitative resistance to Melampsora rust. New Phytologist 197:909–918.
  18. Thomas, W. K., Vida, J. T., Frisse, L. M., Mundo, M., and Baldwin, J. G. 1997. DNA sequences from formalin-fixed nematodes: Integrating molecular and morphological approaches to taxonomy. Journal of Nematology 29:250–254.
  19. Thompson, J. D.,Higgins, D. G., and Gibson, T. J. 1994. CLUSTALW: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22:4673–4680.
  20. Wang, X., Wang, P., Gu, J.-F., Wang, J.-L., and Li, H.-M. 2013. Description of Aphelenchoides xui sp. n. (Nematoda: Aphelenchoididae) in packaging wood from South Africa. Nematology 15:279–289.
  21. Ye, W., Giblin-Davis, R. M., Davies, K. A., Purcell, M. F., Scheffer, S. J., Taylor, G. S.,Center, T. D.,Morris,K., andThomas, W. K. 2007.Molecular phylogenetics and the evolution of host plant associations in the nematode
    genus Fergusobia (Tylenchida: Fergusobiinae). Molecular Phylogenetics and Evolution 45:123–141.
  22. Zeng, Y.-S., Ye, W. M., Giblin-Davis, R. M., Li, C.-H., Du, Z.-J., and Zhao, C. 2010. Schistonchus hirtus n. sp. (Nematoda: Aphelenchoididae), an associate of Ficus hirta in China. Nematology 12:543–556.
  23. Zhao, Z. Q., Davies, K. A., Riley, I. T., and Nobbs, J. M. 2007. Laimaphelenchus heidelbergi sp. nov. (Nematoda: Aphelenchina) from Victoria, Australia, and emendment of the diagnosis of the genus. Transactions of the Royal Society of South Australia, Incorporated 131:182–191.