Morphological and Molecular Characterization of Two Aphelenchoides Endophytic in Poplar Leaves

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Journal of Nematology

Society of Nematologists

Subject: Life Sciences

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ISSN: 0022-300X
eISSN: 2640-396X

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VOLUME 48 , ISSUE 1 (March 2016) > List of articles

Morphological and Molecular Characterization of Two Aphelenchoides Endophytic in Poplar Leaves

LYNN K. CARTA * / SHIGUANG LI / ANDREA M. SKANTAR / GEORGE NEWCOMBE

Keywords : nematode ecology, phylogeny, ribosomal DNA, systematics, taxonomy.

Citation Information : Journal of Nematology. Volume 48, Issue 1, Pages 28-33, DOI: https://doi.org/10.21307/jofnem-2017-006

License : (CC BY 4.0)

Received Date : 30-September-2014 / Published Online: 21-July-2017

ARTICLE

ABSTRACT

During a long-term, large network study of the ecology of plant endophytes in native habitats, various nematodes have been
found. Two poplar species, Populus angustifolia (narrowleaf cottonwood) and Populus trichocarpa (black cottonwood), are important ecological and genomic models now used in ongoing plant–pathogen–endophyte interaction studies. In this study, two different aphelenchid nematodes within surface-sterilized healthy leaves of these two Populus spp. in northwestern North America were discovered. Nematodes were identified and characterized microscopically and molecularly with 28S ribosomal RNA (rRNA) and 18S rRNA molecular markers. From P. angustifolia, Aphelenchoides saprophilus was inferred to be closest to another population of A. saprophilus among sequenced taxa in the 18S tree. From P. trichocarpa, Laimaphelenchus heidelbergi had a 28S sequence only 1 bp different from that of a Portuguese population, and 1 bp different from the original Australian type population. The 28S and 18S rRNA trees of Aphelenchoides and Laimaphelenchus species indicated L. heidelbergi failed to cluster with three other Laimaphelenchus species, including the type species of the genus. Therefore, we support a conservative definition of the genus Laimaphelenchus, and consider these populations to belong to  Aphelenchoides, amended as Aphelenchoides heidelbergi n. comb. This is the first report of these nematode species from within aboveground leaves. The presence of these fungal-feeding nematodes can affect the balance of endophytic fungi, which are important determinants of plant health.

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