Steinernema biddulphi n. sp., a New Entomopathogenic Nematode (Nematoda: Steinernematidae) from South Africa

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Journal of Nematology

Society of Nematologists

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VOLUME 48 , ISSUE 3 (September 2016) > List of articles

Steinernema biddulphi n. sp., a New Entomopathogenic Nematode (Nematoda: Steinernematidae) from South Africa

HARUN CIMEN * / VLADIMI´R PU°zA / JIRI NERMUT / JUSTIN HATTING / TSHIMA RAMAKUWELA / SELCUK HAZIR

Keywords : D2-D3, entomopathogenic nematodes, ITS, new species, phylogeny, South Africa, Steinernema biddulphi, taxonomy

Citation Information : Journal of Nematology. Volume 48, Issue 3, Pages 148-158, DOI: https://doi.org/10.21307/jofnem-2017-022

License : (CC BY 4.0)

Published Online: 21-July-2017

ARTICLE

ABSTRACT

A new species of entomopathogenic nematode (EPN), Steinernema biddulphi n. sp., was isolated from a maize field in Senekal, Free State Province of South Africa. Morphological and molecular studies indicated the distinctness of S. biddulphi n. sp. from other Steinernema species. Steinernema biddulphi n. sp. is characterized IJs with average body length of 663 mm (606–778 mm), lateral fields with six ridges in mid-body region forming the formula 2,6,2. Excretory pore located anterior to mid-pharynx (D% = 46). Hyaline layer occupies approximately half of tail length. Male spicules slightly to moderately curved, with a sharp tip and golden brown in color. The first generation of males lacking a mucron on the tail tip while the second generation males with a short filamentous mucron. Genital papillae with 11 pairs and one unpaired preanal papilla. The new species is further characterized by sequences of the internal transcribed spacer (ITS) and partial 28S regions (D2-D3) of the ribosomal DNA (rDNA). Phylogenetic data show that S. biddulphi n. sp. belongs to the ‘‘bicornutum’’ clade within the Steinernematidae family.

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