Mitochondrial Haplotype-based Identification of Root-knot Nematodes (Meloidogyne spp.) on Cut Foliage Crops in Florida

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Journal of Nematology

Society of Nematologists

Subject: Life Sciences

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ISSN: 0022-300X
eISSN: 2640-396X

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VOLUME 48 , ISSUE 3 (September 2016) > List of articles

Mitochondrial Haplotype-based Identification of Root-knot Nematodes (Meloidogyne spp.) on Cut Foliage Crops in Florida

RICHARD BAIDOO * / SOUMI JOSEPH / TESFAMARIAM M. MENGISTU / JANETE A. BRITO / ROBERT MCSORLEY / ROBERT H. STAMPS / WILLIAM T. CROW

Keywords : cut foliage, endonuclease, identification, mitochondrial haplotype, MORF/MTHIS, mtDNA, PCR-RFLP, restriction enzymes, TRNAH/MRH106

Citation Information : Journal of Nematology. VOLUME 48 , ISSUE 3 , ISSN (Online) 2640-396X, DOI: 10.21307/jofnem-2017-027, September 2016

License : (CC BY 4.0)

Published Online: 21-July-2017

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ABSTRACT

Florida accounts for more than 75% of the national cut foliage production. Unfortunately, root-knot nematodes (RKN) (Meloidogyne spp.) are a serious problem on these crops, rendering many farms unproductive. Currently, information on the Meloidogyne spp. occurring on most commonly cultivated cut foliage crops in Florida, and tools for their rapid identification are lacking. The objectives of this study were to (i) identify specific RKN infecting common ornamental cut foliage crops in Florida and (ii) evaluate the feasibility of using the mtDNA haplotype as a molecular diagnostic tool for rapid identification of large samples of RKN. A total of 200 Meloidogyne females were collected from cut foliage plant roots. Meloidogyne spp. were identified by PCR and RFLP of mitochondrial DNA. PCR and RFLP of mitochondrial DNA were effective in discriminating the Meloidogyne spp. present. Meloidogyne incognita is the most dominant RKN on cut foliage crops in Florida and must be a high target for making management decisions. Other Meloidogyne spp. identified include M. javanica, M. hapla, Meloidogyne sp. 1, and Meloidogyne sp. 2. The results for this study demonstrate the usefulness of the mtDNA haplotype-based designation as a valuable molecular tool for identification of Meloidogyne spp.

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REFERENCES

  1. Adam, M. A. M., Phillips, M. S., and Blok, V. C. 2007. Molecular diagnostic key for identification for single juveniles of seven common and economically important species of root-knot nematode (Meloidogyne spp.). Plant Pathology 56:190–197.
  2. Altschul, S. F., Gish, W., Miller, W., Myers, E. W., and Lipman, D. J. 1990. Basic local alignment search tool. Journal of Molecular Biology 215:403–410.
  3. Baidoo, R., Stamps, R. H., and Crow, W. T. 2014. Pathogenicity and management of Meloidogyne incognita on Pittosporum tobira in Florida [Abstr.]. Journal of Nematology 46:135–136.
  4. Benson, D. M., and Barker, K. R. 1985. Nematodes—A threat to ornamental plants in the nursery and landscape. Plant Disease 69:97–100.
  5. Blok, V. C., Wishart, J., Fargette, M., Berthier, K., and Phillips, M. S. 2002. Mitochondrial DNA differences distinguishing Meloidogyne mayaguensis from the major species of tropical root-knot nematodes. Nematology 4:773–781.
  6. Brito, J. A., Powers, T. O., Mullin, P. G., Inserra, R. N., and Dickson, D. W. 2004. Morphological and molecular characterization of Meloidogyne mayaguensis isolates from Florida. Journal of Nematology 36:232–240.
  7. Brito, J. A., Kaur, R., Cetintas, R., Stanley, J. D., Mendes, M. L., McAvoy, E. J., Powers, T. O., and Dickson, D. W. 2008. Identification and isozyme characterization of Meloidogyne spp. infecting horticultural and agronomic crops, and weed plants in Florida. Nematology 10:757–766.
  8. Brito, J. A., Kaur, R., Cetintas, R., Stanely, J. D., Mendes, M. L., Powers, T. O., and Dickson, D. W. 2010. Meloidogyne spp. infecting ornamental plants in Florida. Nematropica 40:87–103.
  9. Carneiro, R. M. D. G., Almeida, M. R. A., and Queneherve, P. 2000. Enzyme phenotypes of Meloidogyne spp. populations. Nematology 2:645–654.
  10. Esbenshade, P. R., and Triantaphyllou, A. C. 1985. Use of enzyme phenotypes for identification of Meloidogyne species. Journal of Nematology 17:6–20.
  11. Esbenshade, P. R., and Triantaphyllou, A. C. 1987. Enzymatic relationships and evolution in the genus Meloidogyne (Nematoda: Tylenchida). Journal of Nematology 19:8–18.
  12. Esbenshade, P. R., and Triantaphyllou, A. C. 1990. Isozyme phenotypes for the identification of Meloidogyne species. Journal of Nematology 22:10–15.
  13. Fargette, M., Berthier, K., Richaud, M., Lollier, V., Franck, P., Hernandez, A., and Frutos, R. 2010. Crosses prior to parthenogenesis explain the current genetic diversity of tropical plant-parasitic Meloidogyne species (Nematoda: Tylenchida). Infection, Genetics and Evolution 10:807–814.
  14. Handoo, Z. A., Nyczepir, A. P., Esmenjaud, D., van der Beek, J. G., Castagnone-Sereno, P., Carta, L. K., Skantar, A. M., and Higgins, J. A. 2004. Morphological, molecular, and differential-host characterization of Meloidogyne floridensis n. sp. (Nematoda: Meloidogynidae), a root-knot nematode parasitizing peach in Florida. Journal of Nematology 36:20–35.
  15. Hu, M. X., Zhuo, K., and Liao, J. L. 2011. Multiplex PCR for the simultaneous identification and detection of Meloidogyne incognita, M. enterolobii, and M. javanica using DNA extracted directly from individual galls. Phytopathology 101:1270–1277.
  16. H€ubschen, J., Kling, L., Ipach, U., Zinkernagel, V., Brown, D., and Neilson, R. 2004. Development and validation of species-specific primers that provide a molecular diagnostic for virus-vector longidorid nematodes and related species in German viticulture. European Journal of Plant Pathology 110:883–891.
  17. Hugall, A., Moritz, C., Stanton, J., and Wolstenholme, D. R. 1994. Low, but strongly structured mitochondrial DNA diversity in root-knot nematodes (Meloidogyne). Genetics 136:903–912. Hugall, A., Stanton, J., and Moritz, C. 1997. Evolution of the AT-rich mitochondrial DNA of the root-knot nematode, Meloidogyne hapla. Molecular and Biological Evolution 14:40–48.
  18. Hunt, D. J., and Handoo, Z. A. 2009. Taxonomy, identification and principal species. Pp. 55–97 in R. N. Perry, M. Moens, and J. L. Starr, eds. Root-knot nematodes. Wallingford, CT: CAB International.
  19. Janssen, T., Karssen, G., Verhaeven, M., Coyne, D., and Bert, W. 2016. Mitochondrial coding genome analysis of tropical root-knot nematodes (Meloidogyne) supports haplotype based diagnostics and reveals evidence of recent reticulate evolution. Scientific Reports 6:22591. doi: 10.1038/srep22591.
    [CROSSREF]
  20. Jepson, S. B. 1987. Identification of root-knot nematodes (Meloidogyne species). Wallingford, CT: CAB International.
  21. Jeyaprakash, A., Tigano, M. S., Brito, J., Carneiro, R. M. D. G., and Dickson, D. W. 2006. Differentiation of Meloidogyne floridensis from M. arenaria using high-fidelity PCR amplified mitochondrial AT-rich sequences. Nematropica 36:1–12.
  22. Kiewnick, S., Holterman, M., van den Elsen, S., van Megen, H., Frey, J. E., and Helder, J. 2014. Comparison of two short DNA barcoding loci (COI and COII) and two longer ribosomal DNA genes (SSU & LSU rRNA) for specimen identification among quarantine root-knot nematodes (Meloidogyne spp.) and their close relatives. European Journal of Plant Pathology 140:97–110.
  23. Kiewnick, S., Wolf, S., Willareth, M., and Frey, J. E. 2013. Identification of the tropical root-knot nematode species Meloidogyne incognita, M. javanica and M. arenaria using a multiplex PCR assay. Nematology 15:891–894.
  24. Lunt, D. H. 2008. Genetic tests of ancient asexuality in root-knot nematodes reveal recent hybrid origins. BMC Evolutionary Biology 8:194.
  25. Lunt, D. H., Kumar, S., Koutsovoulos, G., and Blaxter, M. L. 2014. The complex hybrid origins of the root-knot nematodes revealed through comparative genomics. PeerJ 2:e356. doi: 10.7717/peerj.356.
  26. McSorley, R., and Marlatt, R. B. 1983. Reaction of Hibiscus rosasinensis cultivars to two species of root knot nematodes. HortScience 18:85–86.
  27. McSorley, R., and Dunn, R. A. 1989. Effects of root-knot nematodes on Areca catechu. Journal of Nematology 21:717–719.
  28. McSorley, R., and Dunn, R. A. 1990. Infection of five species of landscape ornamentals by root-knot nematodes (Meloidogyne spp.). Proceedings of the Soil and Crop Science Society of Florida 49:227–230.
  29. McSorley, R., and Frederick, J. J. 1994. Response of some common annual bedding plants to three species of Meloidogyne. Journal of Nematology 26:773–777.
  30. McSorley, R., and Frederick, J. J. 2001. Host suitability of some vinca and salvia cultivars to two isolates of root-knot nematodes. Proceedings of the Florida State Horticultural Society 114:239–241.
  31. McSorley, R., Wang, K. H., and Frederick, J. J. 2004. Host suitability of Caladium varieties to Meloidogyne incognita. Nematropica 34:97–101.
  32. Moens, M., Perry, R. N., and Starr, J. L. 2009. Meloidogyne species—A diverse group of novel and important plant parasites. Pp. 1–17 in R. N. Perry, M. Moens, and J. Starr, eds. Root-knot nematodes. Wallingford, CT: CABI International.
  33. Nyoike, T. W., Mekete, T., McSorley, R., Weibelzahl-Karigi, E., and Liburd, O. E. 2012. Identification of the root-knot nematode, Meloidogyne hapla, on strawberry in Florida using morphological and molecular methods. Nematropica 42:253–259.
  34. Onkendi, E. M., Kariuki, G. M., Marais, M., and Moleleki, L. N. 2014. The threat of root-knot nematodes (Meloidogyne spp.) in Africa: A review. Plant Pathology 63:727–737.
  35. Pagan, C., Coyne, D., Carneiro, R., Kariuki, G., Luambana, N., Affokpon, A., and Williamson, V. M. 2015. Mitochondrial haplotypebased identification of ethanol-preserved root-knot nematodes from Africa. Phytopathology 105:350–357.
  36. Perfus-Barbeoch, L., Castagnone-Sereno, P., Reichelt, M., Fneich, S., Roquis, D., Pratx, L., Cosseau, C., Grunau, C., and Abad, P. 2014. Elucidating the molecular bases of epigenetic inheritance in non-model invertebrates: The case of the root-knot nematode Meloidogyne incognita. Frontiers in Physiology 5:211. doi: 10.3389/Fphys.2014.00211.
  37. Powers, T. 2004. Nematode molecular diagnostics: From bands to barcodes. Annual Review of Phytopathology 42:367–383.
  38. Powers, T. O., and Harris, T. S. 1993. A polymerase chain reaction method for identification of five major Meloidogyne spp. Journal of Nematology 25:1–6.
  39. Powers, T. O., Mullin, P. G., Harris, T. S., Sutton, L. A., and Higgins, R. S. 2005. Incorporating molecular identification of Meloidogyne spp. into a large scale regional survey. Journal of Nematology 37:226–235.
  40. Qiu, J. J., Westerdahl, B. B., Anderson, C., and Williamson, V. M. 2006. Sensitive PCR detection of Meloidogyne arenaria, M. incognita and M. javanica extracted from soil. Journal of Nematology 38:434–441.
  41. Robertson, L., Dıez-Rojo, M. A., Lopez-Perez, J. A., Piedra Buena, A., Escuer,M., Lopez Cepero, J.,Martınez, C., and Bello, A. 2009. New host races of Meloidogyne arenaria, M. incognita and M. javanica from horticultural regions of Spain. Plant Disease 93:180–184.
  42. Stamps, R. H. 1999. Foliage plants for use as Florists’ ‘‘Greens’’. Apopka, FL: University of Florida.
  43. Stanton, J., Hugall, A., and Moritz, C. 1997. Nucleotide polymorphisms and an (Meloidogyne spp improved PCR-based mtDNA diagnostic for parthenogenetic root-knot nematodes.). Fundamentals of Applied Nematology 20:261–268.
  44. Stanton, J. M., McNicol, C. D., and Steele, V. 1998. Non-manual lysis of second-stage Meloidogyne juveniles for identification of pure and mixed samples based on the polymerase chain reaction. Australian Plant Pathology 27:112–115.
  45. Tamura, K., and Nei, M. 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution 10:512–526.
  46. Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Sudhir, K. 2013. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution 30:2725–2729.
  47. Taylor, A. L., and Sasser, J. N. 1978. Biology, identification, and control of root-knot nematodes (Meloidogyne species). Raleigh, NC: North Carolina State University Graphics.
  48. USDA/NASS. 2014. Floriculture crops 2013 summary. Washington, DC: USDA. Wishart, J., Phillips, M. S., and Blok, V. C. 2002. Ribosomal intergenic spacer: A polymerase chain reaction diagnostic for Meloidogyne chitwoodi, M. fallax, and M. hapla. Phytopatology 92:884–892.
  49. Xu, J., Liu, P., Meng, Q., and Long, H. 2004. Characterization of Meloidogyne species from China using isozyme phenotypes and amplified mitochondrial DNA restriction fragment length polymorphism. European Journal of Plant Protection 110:309–315.
  50. Zijlstra, C., Donkers-Venne, D. T. H. M., and Fargette, M. 2000. Identification of Meloidogyne incognita, M. javanica and M. arenaria using sequence characterised amplified region (SCAR) based PCR assays. Nematology 2:847–845.

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