Description of Enchodorus yeatsi n. sp. (Dorylaimida, Nordiidae) from Southern Iran and Its Molecular Phylogenetic Study

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Journal of Nematology

Society of Nematologists

Subject: Life Sciences

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ISSN: 0022-300X
eISSN: 2640-396X

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VOLUME 49 , ISSUE 1 (March 2017) > List of articles

Description of Enchodorus yeatsi n. sp. (Dorylaimida, Nordiidae) from Southern Iran and Its Molecular Phylogenetic Study

MAJID PEDRAM *

Keywords : 28S rDNA D2/D3, Bayesian, E. neodolichurus, ITS1, Khuzestan province, new species, taxonomy

Citation Information : Journal of Nematology. Volume 49, Issue 1, Pages 21-26, DOI: https://doi.org/10.21307/jofnem-2017-042

License : (CC BY 4.0)

Received Date : 13-August-2016 / Published Online: 21-July-2017

ARTICLE

ABSTRACT

Enchodorus yeatsi n. sp., a new species of the rare genus Enchodorus is described and illustrated based on its morphological and molecular characters. It was recovered from southern Iran. Females of the new species are characterized by having 1,511.3- to 1,792.5-mm long slender body, angular lip region having prominent papillae, 12- to 13-mm long odontostyle, double guiding ring, simple rod-like odontophore, didelphic–amphidelphic reproductive system, and 102- to 160-mm long elongate-conoid tail with rounded tip. Males of the new species are abundant and functional, characterized by 1,484- to 1,576-mm long body, 40- to 43-mm long spicules, 5 to 6 ventromedian supplements, and ventrally bent elongate conical tail. Compared to the type species, Enchodorus dolichurus, the new species has differences in its tail morphology and V value. These morphological differences and the separation of two species was further supported with basic differences in sequences of 28S rDNA D2/D3 and internal transcribed spacer 1 (ITS1) fragments. Compared to Enchodorus neodolichurus, it has basic differences in tail characters and spicule lengths. Molecular phylogenetic studies using partial sequences of 28S rDNA D2/D3 fragment of the new species and available sequences of Nordiidae members and several other dorylaim species/genera, revealed E. yeatsi n. sp. and E. dolichurus forming a clade with 0.81 Bayesian posterior probability (BPP). This clade forms a sister clade to the clade of Heterodorus sp. and Rhyssocolpus vinciguerrae, again with 0.81 BPP. In ITS1 tree, reconstructed using few available sequences, the new species and E. dolichurus formed a clade with 0.98 BPP.

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REFERENCES

  1. Ahmad, W., and Wu, J. 1999. A new species of rare nematode genus Enchodorus Vinciguerra (Nematoda: Dorylaimida) from China. International Journal of Nematology 9:181–184..
  2. Altherr, E. 1952. Les nematodes du Parc National Suisse (Nematodes libres du sol). 2. Ergebnisse der Wissenschaftlichen Untersuchung des Schweizerischen Nationalparks 3:315–356.
  3. Castresana, J. 2000. Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology and Evolution 17:540–552.
  4. De Grisse, A. T. 1969. Redescription ou modifications de quelques techniques utilis_ees dans l’_etude des nematodes phytoparasitaires. Mededelingen Faculteit Landbouwwetenschappen Rijksuniversiteit Gent 34:351–369.
  5. De Man, J. G. 1876. Onderzoekingen over vrij in der aarde levende Nematoden. Tljdschrifi der Nederlandsche Dierkundige Vereeniging 2:78–196.
  6. Edgar, R. C. 2004. MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32:1792–1797.
  7. Jairajpuri, M. S., and Siddiqi, A. H. 1964. On a new nematode genus Nordia (Dorylaimoidea: Nordiinae n. subfam.) with remarks on the genus Longidorella Thorne, 1939. Proceedings of the Helminthological Society of Washington 31:1–9.
  8. Larget, B., and Simon, D. L. 1999. Markov chain Monte Carlo algorithms for the Bayesian analysis of phylogenetic trees. Molecular Biology and Evolution 16:750–759.
  9. Nunn, G. B. 1992. Nematode molecular evolution. Ph.D. dissertation, University of Nottingham, United Kingdom.
  10. Nylander, J. A. 2004. MrModeltest v2. Evolutionary Biology Centre, Uppsala University. http://www.ebc.uu.se/systzoo/staff/nylander.html.
  11. Nylander, J. A., Wilgenbusch, J. C.,Warren, D. L., and Swofford, D. L.2008. AWTY (are we there yet?): A system for graphical exploration of MCMC convergence in Bayesian phylogenetics. Bioinformatics 24:581–583.
  12. Pedram, M., Niknam, G., Guerrero, P., Ye, W., and Robbins, R. T. 2009a. Additional data on Enchodelus veletensis Guerrero, Liebanas & Pe~na-Santiago, 2007 (Dorylaimida: Nordi idae) from Iran, and phylogeny
    of the genus. Nematology 11(2):217–229.
  13. Pedram, M., Niknam, G., Guerrero, P., Ye, W., and Robbins, R. T. 2009b. Morphological and molecular characterisation of Enchodelus babakicus n. sp. and E. macrodorus Thorne, 1939 (Nematoda:Nordiidae) from Iran. Nematology 11(6):895–907.
  14. Pedram, M., Pourjam, E., Robbins, R. T., Ye,W., and Pe~na-Santiago, R.2011. Description of Rhyssocolpus vinciguerrae sp. n. (Dorylaimida, Nordiidae) from Iran and the first molecular study of this genus. Nematology 13:927–937.
  15. Pedram, M., Pourjam, E., Atighi, M. R., and Panahandeh, Y. 2015. Further studies on soil nematode fauna in north western Iran with the description of one new species. Journal of Nematology 47:148–152.
  16. Pe~na-Santiago, R., Abolafia, J., and A lvarez-Ortega, S. 2014. New proposal for a detailed description of the dorylaim spicule (Nematoda: Dorylaimida). Nematology 16:1091–1095.
  17. Pourjam, E., Pedram, M., Vinciguerra, M. T., and Robbins, R. T. 2010.Enchodelus decraemerae n. sp. and Enchodorus dolichurus Vinciguerra, 1976 (Dorylaimida: Nordiidae), two rare species from Iran. Russian
    Journal of Nematology 18:191–197.
  18. Rambaut, A., and Drummond, A. J. 2009. Tracer version 1.5 [computer program]. http://beast.bio.ed.ac.uk.
    Ronquist, F., and Huelsenbeck, J. P. 2003. MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574
  19. Silvestro, D., and Michalak, I. 2011. RaxmlGUI: A graphical front-end for raxML. Organisms Diversity and Evolution 12:335–337.
  20. Subbotin, S. A., Halford, P. D., Warry, A., and Perry, R. 2000. Variations in ribosomal DNA sequences and phylogeny of Globodera parasitising solanaceous plants. Nematology 2:591–604.
  21. Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S.2013. MEGA6: Molecular evolutionary genetics analysis Version 6Vinciguerra, M. T. 1976. Enchodorus, a new genus of Dorylaimoidea from Italy. Nematologica 22:10–14..0. Molecular Biology and Evolution 30:2725–2729.
  22. Whitehead, A. G., and Hemming, J. R. 1965. A comparison of some quantitative methods for extracting small vermiform nematodes from soil. Annals of Applied Biology 55:25–38.

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