Morphological, Morphometric, and Molecular Characterization of Intraspecific Variations within Indian Populations of Meloidogyne graminicola

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Journal of Nematology

Society of Nematologists

Subject: Life Sciences

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ISSN: 0022-300X
eISSN: 2640-396X

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VOLUME 49 , ISSUE 3 (September 2017) > List of articles

Morphological, Morphometric, and Molecular Characterization of Intraspecific Variations within Indian Populations of Meloidogyne graminicola

RAJAN SALALIA * / R. K. WALIA / VISHAL SINGH SOMVANSHI / PUNEET KUMAR / ANIL KUMAR

Keywords : agglomerative hierarchical clustering, diversity, internal transcribed spacer, Meloidogyne graminicola, molecular characterization, morphology, morphometrics, multivariate analysis, phylogenetics, variation.

Citation Information : Journal of Nematology. Volume 49, Issue 3, Pages 254-267, DOI: https://doi.org/10.21307/jofnem-2017-071

License : (CC BY 4.0)

Received Date : 26-March-2017 / Published Online: 03-December-2017

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ABSTRACT

Fourteen populations of Meloidogyne graminicola were collected from different agroecological regions of India. Morphological and morphometrical comparisons were made for various nematode life stages. Three populations (Hisar, New Delhi, and Samastipur) were different from typical M. graminicola on the basis of the length of eggs; J2 length, a-value, hyaline tail portion; male length, distance up to excretory pore, spicule and gubernaculum lengths; female length and width, stylet length, distance up to excretory pore, EPST (distance of excretory pore from anterior end / stylet length [females]) ratio, and vulval length. Morphological and morphometrical comparison with closely related species M. graminis, M. oryzae, M. salasi, M. triticoryzae, and M. lini clustered these populations into two groups: Anand, Bhubaneswar, Hyderabad, Jammu, Jorhat, Kalyani, Kanpur, Ludhiana, Mandya, Palampur, Vellayani grouped with M. graminicola, M. triticoryzae and M. salasi; whereas, Hisar, New Delhi, Samastipur grouped with M. oryzae and M. graminis. Molecular phylogenetic analysis using internal transcribed spacer (ITS) suggested that in spite of morphological differences, these populations belonged to M. graminicola.

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