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Citation Information : Polish Journal of Microbiology. Volume 70, Issue 1, Pages 131-136, DOI: https://doi.org/10.33073/pjm-2021-014
License : (CC-BY-NC-ND 4.0)
Received Date : 18-December-2020 / Accepted: 23-February-2021 / Published Online: 19-March-2021
In dual culture confrontation assays, basidiomycete
Phytopathogenic fungi and oomycetes have caused significant losses in several crop production around the world (Dean et al. 2012; Kamoun et al. 2015). Disease control caused by these pathogens depends to a large extent on agrochemicals, which use has increased worldwide (Carvalho 2017); however, they have accumulated in the trophic chains, affecting wildlife and livestock and causing public health problems (Bourguet and Guillemaud 2016). On the other hand, phytopathogenic microorganisms have increased their resistance to several agrochemicals used to fight them (Sparks and Lorsbach 2017), so they have become less effective. Therefore, the use of alternative plant protection biocontrol methods has been intensively explored during the last decades. Biocontrol methods include both preparations containing living microorganisms and bioactive metabolites obtained from organic or aqueous extracts of different taxa, which may be specific to those that need to be controlled and have low toxicity to wild and human life (Loiseleur 2017).
Previous studies have documented efficient in vitro antagonism of basidiomycete species against phytopathogenic microorganisms, through the use of more than one mechanism (White and Traquair 2006; Gholami et al. 2019). A wide variety of extracts and metabolites obtained from basidiomycete fungi have shown antimicrobial activity, sufficient for growth inhibition of pathogenic microorganisms of medical and agricultural relevance (Shen et al. 2017). Due to the intrinsic ecophysiological role, metabolite production with antimicrobial activity by vegetative mycelium is relevant for species competition; hence, not only antimicrobial activity of biomass extracts but also extracellular filtrates obtained from liquid cultures of vegetative mycelium has been evaluated (Shen et al. 2017). Here we analyzed a wild I. lacteus strain antagonist activity against worldwide relevant phytopathogenic fungi and oomycete species. We also conducted a chemical characterization of extracellular filtrates of such strain aiming to identify putative metabolites with antifungal/antimicrobial activity. The I. lacteus strain CMU-8413 was isolated from basidiocarps collected in September 2013 from the community of Atécuaro, Michoacán, Mexico. Both data on collection and phylogenetic identification of the strain have been previously reported (Damián-Robles et al. 2017). The strain has been deposited in the Laboratorio de Conservación Microbiana y Biotecnología del Centro Multidisciplinario de Estudios en Biotecnología de la Facultad de Medicina Veterinaria y Zootecnia, de la Universidad Michoacana de San Nicolás de Hidalgo, and it may be available for research purposes upon request. The phytopathogenic strains tested correspond to fungi Fusarium pseudocircinatum, Fusarium mexicanum, Colletotrichum coccodes, Colletotrichum gloeosporioides, and oomycetes Phytophthora capsici and Phytophthora cinnamomi. All these phytopathogens have been isolated from crop fields in Michoacán, and they have been properly identified and kindly provided by Dr. Sylvia P. Fernández-Pavía, of the Universidad Michoacana.
Antagonism in dual culture and antibiosis assays were studied according to Steyaert et al. (2016) in three independent replicas for each phytopathogen. The incubation time was adjusted as described below, and incubation temperature to 28°C. The inoculum of I. lacteus and phytopathogens for these assays consisted of a 6 mm plug obtained from the edge of an actively growing colony in PDA at 28°C. For antagonism assays, phytopathogens were inoculated at one extreme of a 90 mm Petri plate containing potato dextrose agar (PDA) medium, and they were incubated until the colony reached a 2 cm radius. At this stage, the PDA Petri plate opposite extreme was inoculated in the same way with the I. lacteus strain, and then incubation was resumed. The fungi colony diameter was measured every 24 h. A respective control of axenic cultures for each phytopathogenic strain was incubated in the same condition. For antibiosis assays, Petri plates containing PDA were covered with sterilized cellophane sheets, and they were inoculated with I. lacteus strain in the center. After a 4-day incubation, the cellophane sheets with the mycelium were aseptically removed. This same PDA plate, on which the I. lacteus strain previously grew, was used for phytopathogens inoculation and incubation in independent assays, and colony diameter was measured every 24 h. Each phytopathogenic strain was inoculated in a fresh PDA medium and was incubated under the same conditions as a control.
For antagonism and antibiosis, assays were finished when the mycelium of each phytopathogen in the control plate had covered 2/3 of the surface area; then, the colony diameter was measured expressing the growth inhibition percentage according to the formula:
% inhibition = [(D1 – D2)/D1 × 100]
where D1 = the phytopathogen colony diameter growing in PDA (fresh/axenic), and D2 = the phytopathogen colony diameter growing in dual culture or in the medium that contained I. lacteus which had been previously incubated.
In antagonism in dual culture assays, I. lacteus showed significant growth inhibition against F. pseudocircinatum (46.3%), F. mexicanum (16.7%), C. coccodes (22.8%), P. cinnamomi (35.0%) and P. capsici (22.9%) (Fig. 1), and it was not able to antagonize C. gloeosporioides. Using the same test, White and Traquair (2006) found that I. lacteus showed efficient growth inhibition of Botrytis cinerea, and this previous work is the only antagonism study between I. lacteus and phytopathogenic fungi, in addition to results reported here. Antibiosis assays showed even better I. lacteus growth inhibitory activity against the six phytopathogenic strains tested, with growth inhibition percentages fluctuating between 32.0 and 86.7%. C. coccodes was the most susceptible species (Fig. 1). The antibiosis assay is used to determine if a fungal strain produces non-volatile metabolites that diffuse into the medium and affect phytopathogenic fungi growth (Steyaert et al. 2016). As far as we could document, there has been no previously published work on antibiosis assays that used basidiomycetes to test their in vitro activity against phytopathogenic fungi/oomycetes.
Antifungal activity of aqueous and organic extracts from basidiocarp and vegetative mycelium of I. lacteus (Shen et al. 2017) had been documented. However, reports evaluating the antifungal activity of extracellular broth filtrates from I. lacteus are scarce; consequently, in this work, it was of particular interest to assess such effect and identifying metabolites secreted by studied strain. The studied strain growth kinetic was conducted in 250 ml Erlenmeyer flasks with 50 ml of potato dextrose broth (PDB) medium, inoculated with six inocula obtained as previously described. The inoculated flasks were incubated at 125 rpm of orbital shaking and 28°C for 14 days. The mycelium dry weight was determined every 24 hours, and the CMU-8413 strain reached the stationary phase after a seven-day incubation (data not shown). Extracellular filtrates were recovered three days after the studied strain reached the stationary phase at the end of 10 days of incubation. Broth culture at the stationary phase was filtered through Whatman No. 1 paper. The filtrate broth was recovered and concentrated by evaporation to dryness in a rotary evaporator at 70°C, without adding any solvent. Concentrates obtained were stored for no more than one week in 1 ml vials at –4°C until biological assays were carried out. Compounds identification in culture filtrate of independent samples for each assay was performed by UPLC (Acquity™ Class I, Waters Corporation) coupled to an orthogonal QTOF mass spectrometer (Synapt™ G1, Waters Corporation), as described elsewhere (Varela-Rodríguez et al. 2019). MS data were continuously acquired and processed with MassLynx® (version 4.1, Waters Corporation), and metabolites were putatively identified with Progenesis® QI for small molecules (Nonlinear Dynamics version 2.3, Waters Corporation) using Chemspider and Progenesis MetaScope as identification methods. The Progenesis QI software for small molecules considers precursor mass accuracy < than 5 ppm, fragmentation pattern, and isotopic similarities, each accounting for 20%. The maximum score is 60% (20 + 20 + 20), pre-identified metabolites have at least 50% of the total score.
A total of 14 extracellular metabolites (Table I) belonging to five chemical groups were found in the I. lacteus broth filtrate. The largest chemical group found was terpenes with nine metabolites, followed by aldehydes with two metabolites. The remaining metabolites found belong to polyketides, quinazolines, and xanthone derivatives, with one metabolite each. All metabolites found in I. lacteus extracellular broths were previously described in other basidiomycete or ascomycete species (Table II). Eight have been described as extracellular in broth media, like here. Terpenes produced by I. lacteus showed antifungal activity against Nigrospora oryzae, C. gloeosporioides, and Didymella glomerata (Wu et al. 2019). It has been previously documented among aldehydes that I. lacteus produces 5-pentenyl-2-furaldehyde, a potent antifungal volatile compound tested against the phytopathogens Fusarium oxysporum f. sp. lycopersici, Colletotrichum fragariae, and B. cinerea (Koitabashi et al. 2004). Also, a frequentin identified here in the I. lacteus broth is an aldehyde derivative that has been previously described as antifungal efficiently inhibiting spore germination in Botrytis allii, Penicillium gladioli, Stachybotrys atra, and Mucor mucedo (Curtis et al. 1951). Finally, microdiplodiasol is a xanthone derivative inhibiting the growth of fungal species Microbotryum violaceum (Siddiqui et al. 2011). Both, previous studies and extracellular metabolites secreted to the broth by the CMU-8413 I. lacteus strain allowed us to anticipate its efficient antagonism and growth inhibition of phytopathogenic fungi and oomycetes.
Phytopathogens growth inhibition assays conducted in solid PDA medium were used as indicative of extracellular inhibitory metabolites by CMU-8413 I. lacteus strain. Based on such results, the identification of the extracellular metabolites was conducted in broth culture to ensure metabolites production in sufficient quantity to be identified by MS. However, it should be noted that the antagonistic and antibiosis assays performed in a solid medium might induce specific metabolite production, which was not produced in broth. In this regard, it has been previously documented that I. lacteus produces the antifungal terpenes conocenol B, 5-demethyl conocenol C, irpenigirin B, as well as 4-(4-dihydroxymethylphenoxy)benzaldehyde only by fermentation in the solid substrate but not in broth culture (Wu et al. 2019). Furthermore, while I. lacteus only produced the first compound in co-culture with the phytopathogen N. oryzae, the remaining three metabolites were produced both in axenic culture and in co-culture. Therefore, extracellular metabolites identified here were not necessarily the same as induced by antagonism in dual culture and antibiosis assays. Co-culture induction of specific metabolites may explain high percentages of fungal/oomycete growth inhibition found here in antagonism/antibiosis assays; however, these findings require further study. Hence, extracellular metabolites produced by the CMU-8413 strain described in this investigation may be considered basal, given that no induction conditions to increase its secretion were evaluated. Based on culture conditions described here, it may be suggested that gene clusters responsible for the synthesis of the identified extracellular metabolites were constitutively expressed or they were not subject to carbon catabolite repression (CCR). CCR and transcription factors associated with secondary metabolism were molecular and metabolic issues scarcely studied in basidiomycetes (Adnan et al. 2018; García-Estrada et al. 2018). Necessary physiological and metabolic studies, like those conducted here, could help to know what kind of metabolites may not be subject to this metabolic control.
It should be noted that extracellular metabolites produced by the CMU-8413 strain have a wide variety of pharmacological activities, besides antimicrobials previously described (Table II). For instance, apotrichodiol and apotrichothecene are neurotoxic mycotoxins (Lebrun et al. 2015), whereas dictyoquinazol A has been described as neuroprotective (Lee et al. 2002) and blennins are inhibitors of leukotriene biosynthesis (Wunder et al. 1996). So, it will be necessary to select favorable culture conditions to develop an enrichment protocol or use heterologous expression systems (Qiao et al. 2019) to obtain the desirable metabolites and avoid toxic molecules’ synthesis. Further studies incubating the CMU-8413 strain in different induction conditions could show its potential to secrete a chemical diversity of metabolites useful for agriculture or other biotechnological applications.
Daisy Pineda-Suazo https://orcid.org/0000-0002-8939-0687
Josaphat Miguel Montero-Vargas https://orcid.org/0000-0002-7471-9152
José Juan Ordaz-Ortiz https://orcid.org/0000-0001-7738-7184
Gerardo Vázquez-Marrufo https://orcid.org/0000-0003-4683-699X
Phytopathogens growth inhibition in dual culture antagonism and antibiosis assays by
Phytopathogens growth inhibition in dual culture antagonism and antibiosis assays by