Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363

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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology

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ISSN: 1733-1331
eISSN: 2544-4646

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VOLUME 66 , ISSUE 3 (September 2017) > List of articles

Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363

Pushpendra Awadhiya / Tushar Banerjee * / Shridhar Patil

Keywords : Delftia acidovorans MTCC 3363, 4-androstene-3, 17-dione, 16-dehydropregnenolone acetate and steroid bioconversion

Citation Information : Polish Journal of Microbiology. VOLUME 66 , ISSUE 3 , ISSN (Online) 2544-4646, DOI: 10.5604/01.3001.0010.4858, September 2017

License : (CC BY-NC-ND 4.0)

Received Date : 09-August-2014 / Accepted: 18-February-2017 / Published Online: 27-September-2017

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ABSTRACT

Delftia acidovorans MTCC 3363 was found to convert 16-dehydropregnenolone acetate (16-DPA) exclusively to 4-androstene-3, 17-dione (AD). Addition of 9α-hydroxylase inhibitors was not required for preventing the accumulation of byproducts. The effect of pH, tempera­ture, substrate concentration, surfactants and carrier solvents on this bioconversion has been studied. 16-DPA was maximally converted in buffered medium at pH 7.0, at temperature 30°C and 0.5 mg ml–1 substrate concentration. Detergent addition and temperature above 35°C had deleterious effect on bioconversion. Dioxan was found to be the best carrier solvent for biotransformation of 16-DPA to AD.

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