Metagenomic Analysis of Soil Bacterial Community and Level of Genes Responsible for Biodegradation of Aromatic Hydrocarbons

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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology

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ISSN: 1733-1331
eISSN: 2544-4646

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VOLUME 66 , ISSUE 3 (September 2017) > List of articles

Metagenomic Analysis of Soil Bacterial Community and Level of Genes Responsible for Biodegradation of Aromatic Hydrocarbons

Jakub Czarny * / Justyna Staninska-Pięta / Jolanta Powierska-Czarny / Jacek Nowak / Łukasz Wolko / Agnieszka Piotrowska-Cyplik

Keywords : aromatic hydrocarbons biodegradation, presence of genes responsible for degradation, soil bacterial community, soil metabiome

Citation Information : Polish Journal of Microbiology. VOLUME 66 , ISSUE 3 , ISSN (Online) 2544-4646, DOI: 10.5604/01.3001.0010.4865, September 2017

License : (CC BY-NC-ND 4.0)

Received Date : 02-May-2016 / Accepted: 04-April-2017 / Published Online: 27-September-2017

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ABSTRACT

The aim of the studies was to compare the composition of soil bacterial metabiomes originating from urbanized areas and areas con­taminated with hydrocarbons with those from agricultural soil and forest soil obtained from a protected wild-life park area. It should be noted that hydrocarbons are everywhere therefore bacteria capable of their utilization are present in every soil type. In the hydrocarbon-contaminated soil and in the soil of anthropogenic origin, the bacteria belonging to Gammaproteobacteria were dominant (28.4–36.6%), whereas in the case of agricultural soil and protected wild-life park soil their ratios decreased (22.8–23.0%) and were similar to that of Alphaproteobacteria. No statistically significant changes were observed in terms of the Operational Taxonomic Unit identified in the studies soils, however, based on the determined alpha-diversity it can be established that contaminated soils were characterized by lower biodiversity indices compared to agricultural and forest soils. Furthermore, the dioxygenase level was also evaluated in the studied soils, which are genes encoding crucial enzymes for the decomposition of mono- and polycyclic aromatic hydrocarbons during the biodegradation of diesel oil (PAHRHDαGN, PAHRHDαGP, xylE, Cat 2,3, ndoB). It was concluded that both the population structure of the soil metabiome and the number of genes crucial for biodegradation processes differed significantly between the soils. The level of analysed genes showed a similar trend, as their highest number in relations to genes encoding 16S RNA was determined in urban and hydrocarbon-contaminated soil.

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