Clonal Analysis of Clinical and Environmental Pseudomonas aeruginosa Isolates from Meknes Region, Morocco


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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology


ISSN: 1733-1331
eISSN: 2544-4646





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VOLUME 66 , ISSUE 3 (September 2017) > List of articles

Clonal Analysis of Clinical and Environmental Pseudomonas aeruginosa Isolates from Meknes Region, Morocco

Itto Maroui * / Abouddihaj Barguigua / Asmae Aboulkacem / Hanane Elhafa / Khadija Ouarrak / Mohammed Sbiti / Lhoussain Louzi / Mohammed Timinouni / Abdelhaq Belhaj

Keywords : Pseudomonas aeruginosa, clonal transmission in hospital, genetic diversity, genotyping of environmental and clinical isolates

Citation Information : Polish Journal of Microbiology. Volume 66, Issue 3, Pages 397-400, DOI:

License : (CC BY-NC-ND 4.0)

Received Date : 21-August-2016 / Accepted: 21-November-2016 / Published Online: 27-September-2017



From 123 clinical and environmental Pseudomonas aeruginosa isolates, 24 strains were selected for their similar antibioresistance, virulence and biofilm formation profiles, to examine their diversity and occurrence of clones within two hospitals and different natural sites in Meknes (Morocco). Pulsed-field gel electrophoresis, using DraI enzyme, didn’t reveal a close relationship between clinical and environmentalisolates nor between strains of the two hospitals. 19 genotypes were obtained, including two virulent environmental clones and three clini­cal clones virulent and resistant to antibiotics. Intra-hospital transmission of high-risk clones detected, in and between wards, constitutes a great public health concern.

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Armour A.D., H.A. Shankowsky, T. Swanson, J. Lee and E.E. Tredget. 2007. The impact of nosocomially-acquired resistant Pseudomonas aeruginosa infection in a burn unit. J. Trauma. 63: 164–171.


Bezuidt O.K., J. Klockgether, S. Elsen, I. Attree, C.F. Davenport and B. Tümmler. 2013. Intraclonal genome diversity of Pseudomonas aeruginosa clones CHA and TB. BMC Genomics 14: 416.


Durmaz R., B. Otlu, F. Koksal, S. Hosoglu, R. Ozturk, Y. Ersoy, E. Aktas, N.C. Gursoy and A. Caliskan. 2009. The optimization of a rapid pulsed-field gel electrophoresis protocol for the typing of Acinetobacter baumannii, Escherichia coli and Klebsiella spp. Jpn J. Infect. Dis. 62: 372–377.


Freitas A.L. and A.L. Barth. 2004. Typing of Pseudomonas aeruginosa from hospitalized patients: a comparison of susceptibility and biochemical profiles with genotype. Braz. J. Med. Biol. Res. 37: 77–82.


Gupta V. 2008. Metallo β-lactamases in Pseudomonas aeruginosa and Acinetobacter species. Expert Opin. Investig. Drugs. 17: 131–143.


Johnson J.K., S.M. Arduino, O.C. Stine, J.A. Johnson and A.D. Harris. 2007. Multilocus sequence typing compared to pulsed-field gel electrophoresis for molecular typing of Pseudomonas aeru-ginosa. J. Clin. Microbiol. 45: 3707–3712.


Kerr K.G. and A.M. Snelling. 2009. Pseudomonas aeruginosa: a formidable and ever-present adversary. J. Hosp. Infect. 73: 338–344.


Landman D., J.M. Quale, D. Mayorga, A. Adedeji, K. Vangala, J. Ravishankar, C. Flores and S. Brooks. 2002. Citywide clonal outbreak of multiresistant Acinetobacter baumanii and Pseudomonas aeruginosa in Brooklyn, NY: the preantibiotic era has returned. Arch. Intern. Med. 162: 1515–1520.


Lim K.T., R.M. Yasin, C.C. Yeo, S.D. Puthucheary, G. Balan, N. Maning, Z. Abdul Wahab, N. Ismail, E.A. Tan, A. Mustaffa and others. 2009. Genetic fingerprinting and antimicrobial susceptibility profiles of Pseudomonas aeruginosa hospital isolates in Malaysia. J. Microbiol. Immunol. Infect. 42: 197–209.


Maatallah M., J. Cheriaa, A. Backhrouf, A. Iversen, H. Grundmann, T. Do, P. Lanotte, M. Mastouri, M.S. Elghmati, F. Rojo and others. 2011. Population structure of Pseudomonas aeruginosa from five Mediterranean countries: Evidence for frequent recombination and epidemic occurrence of CC235. PloS One 6: e25617.


Maroui I., A. Barguigua, A. Aboulkacem, K. Ouarrak, M. Sbiti, H. Louzi, M. Timinouni and A. Belhaj. 2016a. First report of VIM-2 metallo-β-lactamases producing Pseudomonas aeruginosa isolates in Morocco. J. Infect. Chemother. 22: 127–132.


Maroui I., A. Aboulkacem, M. Timminouni and A. Belhaj. 2016b. Virulence profiles of clinical and environmental Pseudomonas aeruginosa isolates from Central Morocco. Afr. J. Microbiol. Res. 10: 473–480.


Mathee K., G. Narasimhan, C. Valdes, X. Qiu, J.M. Matewish, M. Koehrsen, A. Rokas, C.N. Yandava, R. Engels, E. Zeng and others. 2008. Dynamics of Pseudomonas aeruginosa genome evolution. Proc. Natl. Acad. Sci. USA 105: 3100–3105.


Muller M.P. and M. Gubina. 2000. Serotype, antimicrobial susceptibility and clone distribution of Pseudomonas aeruginosa in a university hospital. Zentralbl. Bakteriol. 289: 857–867.


Picard B., E. Denamur, A. Barakat, J. Elion and P. Goullet. 1994. Genetic heterogenicity of Pseudomonas aeruginosa clinical isolates revealed by esterase electrophoretic polymorphism and restriction fragment polymorphism of the ribosomal RNA gene region. J. Med. Microbiol. 40: 313–322.


Pirnay J.P., F. Bilocq, B. Pot, P. Cornelis, M. Zizi, J. Van Eldere, P. Deschaght, M. Vaneechoutte, S. Jennes, T. Pitt and others. 2009. Pseudomonas aeruginosa population structure revisited. PLoS One 4: e7740.


Romling U., B. Fiedler, J. Bosshammer, D. Grothues, J. Greipel, H. Von der Hardt and B. Tummler. 1994b. Epidemiology of chronic Pseudomonas aeeruginosa infections in cystic fibrosis. J. Infect. Dis. 170: 1616–1621.


Romling U., J. Wingender, H. Muller and B. Tummler. 1994a. A major Pseudomonas aeruginosa clone common to patients and aquatic habitats. Appl. Environ. Microbiol. 60: 1734–1738.


Selim S., I. El Kholy, N. Hagagy, S. El Alfay and M. Abdel Aziz. 2015. Rapid identification of Pseudomonas aeruginosa by pulsed-field gel electrophoresis. Biotechnol. Biotechnol. Equip. 29: 152–156.


Sommerfeld Ross S. and J. Fiegel. 2012. Nutrient dispersion enhances conventional antibiotic activity against Pseudomonas aeruginosa biofilms. Int. J. Antimicrob. Agents 40: 177–181.


Speert D.P. 2002. Molecular epidemiology of Pseudomonas aeruginosa. Front. Biosci. 1: 354–361.


Wiehlmann L., G. Wagner, N. Cramer, B. Siebert, P. Gudowius P, G. Morales, T. Kohler, C. van Delden, C. Weinel, P. Slickers and others. 2007. Population structure of Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. USA 104: 8101–8106.


Yousefi S., M.B. Nahaei, S. Farajnia, M. Aghazadeh, A. Iversen, P. Edquist, M. Maatallah and C.G. Giske. 2013. A multiresistant clone of Pseudomonas aeruginosa sequence type 773 spreading in a burn unit in Orumieh. Iran. APMIS 121: 146–152.