Characterization of Bacteriocin-Producing Lactic Acid Bacteria Isolated from Native Fruits of Ecuadorian Amazon

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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology

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ISSN: 1733-1331
eISSN: 2544-4646

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VOLUME 66 , ISSUE 4 (December 2017) > List of articles

Characterization of Bacteriocin-Producing Lactic Acid Bacteria Isolated from Native Fruits of Ecuadorian Amazon

Karina Garzón / Clara Ortega / Gabriela N. Tenea *

Keywords : antimicrobial substances, bacteriocins, foodborne pathogens, lactic acid bacteria (LAB)

Citation Information : Polish Journal of Microbiology. VOLUME 66 , ISSUE 4 , ISSN (Online) 2544-4646, DOI: 10.5604/01.3001.0010.7037, December 2017

License : (CC BY-NC-ND 4.0)

Received Date : 18-February-2017 / Accepted: 17-May-2017 / Published Online: 04-December-2017

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ABSTRACT

Tropical, wild-type fruits are considered biodiverse “hotspots” of microorganisms with possible functional characteristics to be investigated. In this study, several native lactic acid bacteria (LAB) of Ecuadorian Amazon showing highly inhibitory potential were identified and characterized. Based on carbohydrate fermentation profile and 16S rRNA gene sequencing, seven strains were assigned as Lactobacillus plantarum and one strain as Weissella confusa. Using agar-well diffusion method the active synthetized components released in the neu­tralized and hydroxide peroxide eliminated cell-free supernatant were inhibited by proteolytic enzymes, while the activity was maintained stable after the treatment with catalase, lysozyme, α-amylase and lipase suggesting their proteinaceous nature. The inhibitory activity was stimulated by acidic conditions, upon exposure to high heat and maintained stable at different ranges of sodium chloride (4–10%). The DNA sequencing analysis confirmed the presence of plw structural gene encoding for plantacirin W in the selected L. plantarum strains. Moreover, we showed that the active peptides of Cys5-4 strains contrast effectively, in a bactericidal manner, the growth of food borne E. coli UTNEc1 and Salmonella UTNSm2, with about tree fold reduction of viable counts at the early stage of the target cell growth. The results indicated that the bacteriocin produced by selected native lactic acid bacteria strains has elevated capacity to suppress several pathogenic microorganisms implying their potential as antimicrobial agents or food preservatives.

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REFERENCES

Ali W.S. and R.M. Musleh. 2015. Purification and characterization of plantacirin vgw8, a bacteriocin produced by Lactobacillus plantarum VGW8. J. Biol. 5(1): 147–152.

 

Arena M.P., A. Silvain, G. Normanno, F. Grieco, D. Drider, G. Spano, and D. Fiocco. 2016. Use of Lactobacillus plantarum strains as a bio-control strategy against food-borne pathogenic microorganisms. Front. Microbiol. 7(464): 1–10.

 

Atrih A., N. Rekhif, A.J.G. Moir, A. Lebrihi and G. Lefebvre 2001. Mode of action, purification and amino acid sequence of plantacirin C19, an anti-Listeria bacteriocin produced by Lactobacillus plantarum C19. Int. J. Food Microbiol. 68: 93–109.

 

Banerjee S.P., K.C. Dora and S. Chowdhury. 2013. Detection, partial purification and characterization of bacteriocin produced by Lactobacillus brevis FPTLB3 isolated from freshwater fish. J. Food Sci. Technol. 50: 17–25.

 

Benavidez A.B., M. Ulcuango, L. Yepez, and G.N. Tenea. 2016. Assessment of the in vitro bioactive properties of lactic acid bacteria isolated from native ecological niches of Ecuador. Rev. Argent. Microbiol. 48: 236–244.

 

Collado M., J. Meriluoto, and S. Salminen. 2007 Role of commercial probiotic strains against human pathogen adhesion to intestinal mucus. Lett. Appl. Microbiol. 45: 454–460.

 

Corsetti A., L. Settanni and D. van Sinderen. 2004. Characterization of bacteriocin-like inhibitory substances (BLIS) from sourdough lactic acid bacteria and evaluation of their in vitro and in situ activity. J. Appl. Microbiol. 96: 521–534.

 

Danielsen, M. and A. Wind A. 2003. Susceptibility of Lactobacillus spp. to antimicrobial agents. Int. J. Food Microbiol. 82: 1–11.

 

da Silva Sabo S., M. Vitolo, J.M.D. Gonzalez and R.P. de Souza Oliveira. 2014. Overview of the Lactobacillus plantarum as a promising bacteriocin producer among lactic acid bacteria. Food Res. Int. 64: 527–536.

 

Deegan L.H., P.D. Cotter, C. Hill and P. Ross. 2006. Bacteriocins: Biological tools for bio-preservation and shelf-life extension. Int. Dairy J. 16: 1058–1071.

 

De Man J.C., M. Rogosa and E. Sharpe. 1960. A medium for the cultivation of lactobacilli. J. Appl. Bacteriol. 23: 130–155.

 

Deraz S.F., E.N. Karlsson, A.A. Khalil and B. Mattiasson. 2007. Mode of action of acidocin D20079, a bacteriocin produced by the potential probiotic strain, Lactobacillus achidophilus DSM 20079.J. Ind. Micobiol. Biotechnol. 34: 34373–379.

 

Galvez A., H. Abriouel, R.L. Lopez and B.N. Omar. 2007. Bacteriocin-based strategies for food biopreservation. Int. J. Food Microbiol. 120: 51–70.

 

Gaona J. 2013. Ministerio de Salud Pública del Ecuador. https://public.tableau.com/profile/javier.gaona#!/vizhome/MORBILIDAD_ RDACAA_2013_0/Presentacin.

 

Georgieva R., I. Iliev, T. Haertle, J.M. Chobert, I. Ivanova and S. Danova. 2009. Technological properties of candidate probiotic Lactobacillus plantarum strains. Int. Dairy J. 19: 696–702.

 

Goh H.F. and K. Philip. 2015. Purification and characterization of bacteriocin produced by Weissella confusa A3 of dairy origin. PLOS One. 10: e0140434.

 

Halo H., Z. Jeknic, M. Daeschel, S. Stevanovic and I.F. Nes. 2001.Plantacirin W from Lactobacillus plantarum belongs to a new family of two-peptide lantibiotics. Microbiology 147: 643–651.

 

Hernandez D., E. Cardell and V. Zarate. 2005. Antimicrobial activity of lactic acid bacteria isolated from Tenerife cheese: initial characterization of plantaricin TF711, a bacteriocin-like substance produced by Lactobacillus plantarum TF711. J. Appl. Microbiol. 99: 77–84.

 

Hiramoto S., K. Itoh, S. Shizuuchi, Y. Kawachi, Y. Morishita, M. Nagase, Y. Suzuki, Y. Nobuta, O. Nakamura, I. Kagaya and others. 2004. Melanoidin, a food protein-derived advanced Maillard reaction product, suppresses Helicobacter pylori in vitro and in vivo. Helicobacter. 9: 429–435.

 

Jimenez-Diaz R., R.M. Rios-Sanchez, M. Desmazeaud, J.L. Ruiz-Barba and J.C. Piard. 1993. Plantaricins S and T, two new bacteriocins produced by Lactobacillus plantarum LPCO10 isolated froma green olive fermentation. Appl. Environ. Microbiol. 59: 1416–1424.

 

Longdet I.Y., R.J. Kutdhik and I.G. Nwoyeocha. 2011. The probiotic efficacy of Lactobacillus casei from human breast milk against shigellosis in albino rats. Advances Biotech. Chem. Proc. 1: 12–16.

 

Mahrous H., A. Mohamed, M. Abd El-Mongy, A.I. El-Batal and H.A. Hamza. 2013. Study bacteriocin production and optimization using new isolates of Lactobacillus ssp. isolated from some dairy products under different culture conditions. Food Nutr. Sci. 4: 342–356.

 

Nes I.F. and O. Johnsborg. 2004 Exploration of antimicrobial potential of LAB by genomics. Curr. Opin. Biotech. 15: 100–104.

 

Noonpakdee W., P. Jumriangrit, K. Wittayakom, J. Zendo, J. Nakayama, K. Sonomoto, and S. Panyim. Two-peptide bacteriocin from Lactobacillus plantarum PMU 33 strain isolated fromsom-fak, a Thai low salt fermented fish product. As. Pac. J. Mol. Biol. Biotech. 17: 19–25.

 

O’Shea, E., P. Cotter, C. Stanton, R. Ross and C. Hill. 2011. Production of bioactive substances by intestinal bacteria as a basis for explaining probiotic mechanism: bacteriocins and conjugated linoleic acid. Int. J. Food Microbiol. 152: 189–205.

 

Reis J.A., A.T. Paula, S.N. Casarotti, and A.I.B. Penna. 2012. Lactic acid bacteria antimicrobial compounds: characteristics and applications. Food Eng. Rev. 4: 124–140.

 

Tenea G.N. and L. Yépez. 2016. Bioactive Compounds of Lactic Acid Bacteria. Case Study: Evaluation of Antimicrobial Activity of Bacteriocin-Producing Lactobacilli Isolated from Native Ecological Niches of Ecuador, pp. 147–169. In: Rao V. and L.G. Rao (eds.) Probiotics and prebiotics in human nutrition and health, InTech.

 

Todorov S.D. 2008. Bacteriocin production by Lactobacillus plantarum AMA-K isolated from amasi, a Zimbabwean fermented milk product and study of the adsorption of bacteriocin AMA-K toListeria sp. Brazilian J. Microbiol. 39: 178–187.

 

Todorov S.D., C. Rachman, A. Fourrier, L.M.T. Dicks C.A. van Reenen, H. Prevost and X. Dousset. 2011. Characterization of a bacteriocins produced by Lactobacillus sakei R1333 isolated from smoked salmon. Anaerobe 17: 23–31.

 

Todorov S.D., M. Vaz-Velho, G. de Melo, B.D. Franco andW.H. Holzapfel. 2013. Partial characterization of a bacteriocin produced by three strains of Lactobacillus sakei isolated from salpicao, a fermented meat product from North-West of Portugal. Food Control. 30: 111–121.

 

Trang V.T., V.H. Son, L.X. Thanh, S. Sarter, T. Shimamura,H. Uked. and H. Takeurchi. 2013. Functional properties of Maillard reaction products in food: Antimicrobial activity of aminoreductone against pathogenic bacteria. Food Sci. Technol. Res. 19: 833–841.

 

Yi H., L. Zhang, Y. Tuo, X. Han and M. Du. 2010. A novel method for rapid detection of class IIa bacteriocin-producing lactic acid bacteria. Food Control. 21: 426–430.

 

Zambou N.F., P.M. Kaktcham, A.H.N. Tiogo and W.R.E. Guetiya. 2013. Antimicrobial activity of a bacteriocin produced by Lactobacillus plantarum 29V and strain’s viability in palm kernel oil. Int.J. Nutri. Food Sci. 2: 102–108.

 

Zendo T. 2013. Screening and Characterization of novel bacteriocins from lactic acid bacteria. Biosci. Biotechnol. Biochem. 77: 839–899.

 

Zendo T., N. Eungruttanagorn, S. Fujioka, Y. Tashiro, K. Nomura, Y. Sera, G. Kobayashi, J. Nakayama, A. Ishizaki, and K. Sonomoto. 2005. Identification and production of a bacteriocin from Enterococcus mundtii QU 2 isolated from soybean. J. Appl. Microbiol. 99: 1181–1190.

 

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