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Citation Information : Polish Journal of Microbiology. VOLUME 66 , ISSUE 4 , ISSN (Online) 2544-4646, DOI: 10.5604/01.3001.0010.7043, December 2017
License : (CC BY-NC-ND 4.0)
Received Date : 03-March-2017 / Accepted: 13-June-2017 / Published Online: 04-December-2017
Over a period of three years, microbial communities in acidified soil with high sulfur content were analyzed. In soil water extracts ureolytic, proteolytic, oxidoreductive, and lipolytic activity were detected. The presented results indicate that the enzymatic activity of soil microbial communities varied considerably over time. Isolated 26 (80%) bacterial strains belonged to genus Bacillus sp. and were identified bycultivation and 16S rRNA methods. The commercially available procedures for bacterial DNA isolation from acidified soil failed, therefore a new, specific DNA isolation method was established. Ureolytic activity, detected in soil extracts as well as in isolated Bacillus sp. strains may be considered as a tool for the bioremediation of acidified soils with high sulfate content.
Blagodatskaya E. and Y. Kuzyakov. 2013. Active microorganisms in soil: Critical review of estimation criteria and approaches. Soil Biol. Biochem. 67: 192–211.
Cui P., F. Fan Ch. Yin, Z. Li, A. Song, Y. Wan and Y. Liang. 2013. Urea- and nitrapyrin-affected N2O emission is coupled mainly with ammonia oxidizing bacteria growth in microcosms of three typical Chinese arable soils. Soil Biol. Biochem. 66: 214–221.
van Elsas J.D., R. Costa, J. Jansson, S. Sjöling, M. Bailey, R. Nalin, T.M. Vogel and L. van Overbeek. 2008. The metagenomics ofdisease-suppressive soils – experiences from the METACONTROL project. Trends Biotechnol, 26: 591–601.
Ettema C.H. and D.A. Wardle. 2002. Spatial soil ecology. Trends Ecol. Evol. 17: 177–183.
Fierer N. and R.B. Jackson. 2006. The diversity and biogeographyof soil bacterial communities. Proc. Natl. Acad. Sci USA 103: 626–631.
Gąsiewicz A., M. Jasionowski and A. Poberzhskyy. 2012. Influence of sulphur exploitation on features of geochemical surface on Polish-Ukraine border (in Polish). Biuletyn Panstowego Instytutu Geologicznego 449: 5–40.
González, V., I. García, F. del Moral, S. de Haro, J.A. Sánchez and M. Simón. 2011. Impact of unconfined sulphur-mine waste on a semi-arid environment (Almería, SE Spain). J. Environ. Manage. 92: 1509–1519.
Kang C.-H. Soo Ji Oh, Y.J. Shin, S.-H. Han, I.-H. Nam and J.-S. So. 2015. Bioremediation of lead by ureolytic bacteria isolated from soil at abandoned metal mines in South Korea. Ecological Engineering, 74: 402–407.
Krzywy-Gawrońska E. 2012. Enzymatic activity of urease and dehydrogenase in soil fertilized with GWDA compost with or without a PRPSOL addition. Pol. J. Environ. Stud. 21: 949–955.
Kumar S., S. Chaudhuri and S.K. Maiti. 2013. Soil dehydrogenase enzyme activity in natural and mine Soil – A Review. Middle-East J. Sci. Res. 13: 898–906.
Li X.D., H. Masuda, M. Kusakabe, F. Yanagisawa and H.-A. Zeng. 2006. Degradation of groundwater quality due to anthropogenic sulfur and nitrogen contamination in the Sichuan Basin, China. Geochemical Journal, 40: 309–332.
Martyn W., T. Wyłupek, J. Onuch-Amborska and M. Jońca. 2004. The effects of sulphur mining on the soil in the area of the former sulphur mine Basznia near Lubaczow (Poland). Annales UMCSSectio E, 59: 1407–1414.
Mols M. and T. Abee. 2008. Role of ureolytic activity in Bacillus cereus nitrogen metabolism and acid survival. Appl. Environ. Microbiol. 74: 2370–2378.
Muyzer G., E.C. de Waal. and A G. Uitterlinden. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl. Environ. Microbiol. 59: 695–700.
Ranjard L., F. Poly, and J. Combrisson. 2000. Heterogeneous cell density and genetic structure of bacterial pools associated withvarious soil microenvironments as determined by enumeration and DNA fingerprinting approach (RISA). Microb. Ecol. 39: 263–272.
Rousk, J., E. Bååth, P.C. Brookes, C.L. Lauber, C. Lozupone,J.G. Caporaso, R. Knight and N. Fierer. 2010. Soil bacterial and fungal communities across a pH gradient in an arable soil. ISME J.4: 1340–1351.
Rousk J., P.C. Brookes and E. Bååth. 2009. Contrasting soil pH effects on fungal and bacterial growth suggest functional redundancy in carbon mineralization. Appl. Environ. Microbiol. 75: 1589–1596.
Schwieger F. and C.C. Tebbe. 1998. A new approach to utilize PCR-single-strand-conformation polymorphism for 16S rRNA gene-based microbial community analysis. Appl. Environ. Microbiol. 64: 4870–4876.
Shirakawa M.A., K.K. Kaminishikawahara, V.M. John, H. Kahn and M.M. Futai. 2011. Sand bioconsolidation through the precipitation of calcium carbonate by two ureolytic bacteria. Mater. Lett. 65: 1730–1733.
Sołek-Podwika K. and K. Ciarkowska. 2012. Properties of soils from the places where sulphur was stored. Soil Science Annual. 63: 46–48.
Tsai Y.L. and B.H. Olson. 1991. Rapid method for direct extraction of DNA from soil and sediments. Appl. Environ. Microbiol. 57: 1070–1074.
Wang T., H. Sun, Ch. Jiang, , H. Mao and Y. Zhang. 2014. Immobilization of Cd in soil and changes of soil microbial community by bioaugmentation of UV-mutated Bacillus subtilis 38 assisted by biostimulation. Eur. J. Soil Biol. 65: 62–69.
Valenzuela L., A. Chi, S. Beard, A. Orell, N. Guiliani, J. Shabanowitz, D.F. Hunt and C.A. Jerez. 2006. Genomics, metagenomics and proteomics in biomining microorganisms. Biotechnol. Adv. 24: 197–211.
Zieliński A. and G. Wałek. 2012. Changes in georafical enviroments in Grzybow region (Niecka Nidzianska) in 1900–2000 based on topopographical maps (in Polish). 19: 103–109.
Xu M., X. Li, X. Cai , J. Gai X. Li, P. Christie and J. Zhang.2014. Soil microbial community structure and activity along a montane elevational gradient on the Tibetan Plateau. Eur. J. Soil Biol. 64: 6–14.