Inhibition of Multidrug-Resistant Gram-Positive and Gram-Negative Bacteria by a Photoactivated Porphyrin


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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology


ISSN: 1733-1331
eISSN: 2544-4646





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VOLUME 66 , ISSUE 4 (December 2017) > List of articles

Inhibition of Multidrug-Resistant Gram-Positive and Gram-Negative Bacteria by a Photoactivated Porphyrin

Moreno Bondi / Anna Mazzini / Simona De Niederhäusern * / Ramona Iseppi / Patrizia Messi

Keywords : antibiotic-resistant bacteria, laser light irradiation, porphyrin, photo-activation treatment

Citation Information : Polish Journal of Microbiology. Volume 66, Issue 4, Pages 533-536, DOI:

License : (CC BY-NC-ND 4.0)

Received Date : 21-November-2016 / Accepted: 12-January-2017 / Published Online: 04-December-2017



The authors studied the in vitro antibacterial activity of the photo-activated porphyrin meso-tri(N-methyl-pyridyl), mono(N-tetradecyl-pyridyl)porphine (C14) against four multidrug-resistant bacteria: Staphylococcus aureus, Enterococcus faecalis (Gram-positive), Escherichia coli, Pseudomonas aeruginosa (Gram-negative). Using 10 μg/ml of porphyrin and 60 sec irradiation we observed the remarkable suscep­tibility of S. aureus and E. faecalis to treatment while, under the same conditions, E. coli and P. aeruginosa showed very low susceptibility.In a later stage, suspensions of Gram-negative bacteria were processed with EDTA before photo-activation, obtaining a significant decrease in viable counts. In view of the results, if the combination of low porphyrin concentrations and short irradiation times will be effectivein vivo also, this approach could be a possible alternative to antibiotics, in particular against localized infections due to multidrug-resistant microorganisms.

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Dai T., Y. Huang and M. Hamblin. 2009. Photodynamic therapy for localized infections-State of the art. Photodiagnosis Photodyn. Ther. 6: 170–188


Dantes R., Y. Mu, R. Belflower, D. Aragon, G. Dumyati, L.H. Harrison, F.C. Lessa, R. Lynfield J., Nadle, S. Petit et al. 2013. National burden of invasive methicillin-resistant Staphylococcus aureus infec-tions, United States. JAMA Intern. Med. 173: 1970–1978.


de Niederhausern S., M. Bondi, P. Messi, R. Iseppi, C. Sabia,G. Manicardi and I. Anacarso. 2011. Vancomycin-resistance transferability from VanA enterococci to Staphylococcus aureus. Curr. Microbiol. 62: 1363–1367.


de Niederhausern S., C. Sabia, P. Messi, E. Guerrieri, G. Manicardi and M. Bondi. 2007. VanA-type vancomycin-resistant enterococci in equine and swine rectal swabs and in human clinical samples. Curr. Microbiol. 55: 240–246.


Huang L., T. Dai and M.R. Hamblin. 2010. Antimicrobial photodynamic inactivation and photodynamic therapy for infections. Methods Mol. Biol. 635: 155–173.


Jori G., C. Fabris, M. Soncin, S. Ferro, O. Coppellotti, D. Dei,L. Fantetti, G. Chiti and G. Roncucci. 2006. Photodynamic therapy in the treatment of microbial infections: Basic principles and perspective applications. Lasers Surg. Med. 38: 468–481.


Lazzeri D., M. Rovera, L. Pascual and E.N. Durantini. 2004. Photodynamic studies and photoinactivation of Escherichia coli using meso-substituted cationic porphyrin derivatives with asymmetric charge distribution. J. Photochem. Photobiol. 80: 286–293.


Magaraggia M., F. Faccenda, A. Gandolfi and G. Jori. 2006. Treatment of microbiologically polluted aquaculture waters by a novel photochemical technique of potentially low environmental impact. J. Environ. Monitor 8: 923–931.


Merchat M., G. Bertolini, P. Giacomini, A. Villanueva and G. Jori. 1996a. Meso-substituted cationic porphyrins as efficient photosensitizers of Gram-positive and Gram-negative bacteria J. Photochem. Photobiol. 32: 153–157.


Merchat M., J.D. Spikes, G. Bertoloni and G. Jori. 1996b. Studies on the mechanism of bacteria photosensitization by meso-substituted cationic porphyrins. J. Photochem. Photobiol. 35: 149–153.


National Nosocomial Infections Surveillance System. 2004. National Nosocomial Infections Surveillance (NNIS) System report, data summary from January 1992 through June 2004, issued October 2004. Am. J. Infect. Control. 32: 470–485.


Reddi E., M. Ceccon, G. Valduga, G. Jori, J.C. Bommer, F. Elisei,L. Latterini and U. Mazzucato. 2002. Photophysical propertiesand antibacterial activity of meso-substituted cationic porphyrins. Photochem. Photobiol. 75: 462–470.


Shaikh S., J. Fatima, S. Shakil, S.M.D. Rizvi and M.A. Kamal. 2015. Antibiotic resistance and extended spectrum beta-lactamases: types, epidemiology and treatment. Saudi J. Biol. Sci. 22: 90–101.


Sievert D. M., M.L. Boulton, G. Stoltman, D. Johnson, M.G. Stobierski, W. Brown, W. Hafeez and T. Lundstrom. 2002. Staphylococcus aureus resistant to vancomycin-United States. MMWR Morb. Mortal. Wkly. Rep. 51: 565–567.


Tavares A.1., C.M. Carvalho, M.A. Faustino, M.G. Neves,J.P. Tomé, A.C. Tomé, J.A. Cavaleiro, A. Cunha, N.C. Gomes,E. Alves and A. Almeida. 2010. Antimicrobial photodynamic therapy: study of bacterial recovery viability and potential development of resistance after treatment. Mar. Drugs. 8: 91–105.


Wainwright M. and K.B. Crossley. 2004. Photosensitizing agents circumventing resistance and breaking down biofilms: a review. Int. Biodeterior. Biodegrad. 53: 119–126.