Inhibition of Multidrug-Resistant Gram-Positive and Gram-Negative Bacteria by a Photoactivated Porphyrin

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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology

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ISSN: 1733-1331
eISSN: 2544-4646

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VOLUME 66 , ISSUE 4 (December 2017) > List of articles

Inhibition of Multidrug-Resistant Gram-Positive and Gram-Negative Bacteria by a Photoactivated Porphyrin

Moreno Bondi / Anna Mazzini / Simona De Niederhäusern * / Ramona Iseppi / Patrizia Messi

Keywords : antibiotic-resistant bacteria, laser light irradiation, porphyrin, photo-activation treatment

Citation Information : Polish Journal of Microbiology. VOLUME 66 , ISSUE 4 , ISSN (Online) 2544-4646, DOI: 10.5604/01.3001.0010.7092, December 2017

License : (CC BY-NC-ND 4.0)

Received Date : 21-November-2016 / Accepted: 12-January-2017 / Published Online: 04-December-2017

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ABSTRACT

The authors studied the in vitro antibacterial activity of the photo-activated porphyrin meso-tri(N-methyl-pyridyl), mono(N-tetradecyl-pyridyl)porphine (C14) against four multidrug-resistant bacteria: Staphylococcus aureus, Enterococcus faecalis (Gram-positive), Escherichia coli, Pseudomonas aeruginosa (Gram-negative). Using 10 μg/ml of porphyrin and 60 sec irradiation we observed the remarkable suscep­tibility of S. aureus and E. faecalis to treatment while, under the same conditions, E. coli and P. aeruginosa showed very low susceptibility.In a later stage, suspensions of Gram-negative bacteria were processed with EDTA before photo-activation, obtaining a significant decrease in viable counts. In view of the results, if the combination of low porphyrin concentrations and short irradiation times will be effectivein vivo also, this approach could be a possible alternative to antibiotics, in particular against localized infections due to multidrug-resistant microorganisms.

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