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Citation Information : Polish Journal of Microbiology. VOLUME 66 , ISSUE 2 , ISSN (Online) 2544-4646, DOI: 10.5604/01.3001.0010.7869, June 2017
License : (CC BY-NC-ND 4.0)
Received Date : 05-August-2016 / Accepted: 12-January-2017 / Published Online: 28-June-2017
Several studies have observed that use of a conventional PCR protocol with primers LM1 and LM2 for the identification of the hlyA gene of Listeria monocytogenes generates non-specific PCR amplifications and false positives. For this reason, in this study we provide a modified PCR protocol that improves the specificity of the results obtained with LM1 and LM2 primers.
Aznar R. and B. Alarcón. 2002. On the specificity of PCR detection of Listeria monocytogenes in food: a comparison of published primers. Syst. Appl. Microbiol. 25: 109–119.
Aznar R. and B. Alarcón. 2003. PCR detection of Listeria monocytogenes: a study of multiple factors affecting sensitivity. J. Appl. Microbiol. 95: 958–966.
Aznar R. and P. Elizaquível. 2008. Reliability of Listeria monocytogenes by specific PCR assessed by phenotypic and genotypic techniques. Food Anal. Methods. 1: 243–251.
Aznar R. and I. Solís. 2006. PCR detection of Listeria monocytogenes in different food products compared with the mini-VIDAS LMO System and the Standard Procedure ISO 11290-1. J. Verbr. Lebensm. 1: 115–120.
Border P.M., J.J. Howard, G.S. Plastow and K.W. Siggens. 1990. Detection of Listeria species and Listeria monocytogenes using polymerase chain reaction. Lett. Appl. Microbiol. 11: 158–162.
Brankica L., S. Aleksandra, Milijasevic, M. Terzic-Vidojevic, N. Golic and L. Topisirvoic. 2010. The presence of Listeria spp. and Listeria monocytogenes in a chosen food processing establishment in Serbia. Arch. Biol. Sct. Beorgr. 62: 881–887.
Elizaquível P., J.A. Gabaldón and R. Aznar. 2011. Quantification of Salmonella spp., Listeria monocytogenes and Escherichia coli O157:H7 in non-spiked food products and evaluation of real-time PCR as a diagnostic tool in routine food analysis. Food Control. 22: 158–164.
Jamali H., L.C. Chai and K.L. Thong. 2013. Detection and isolation of Listeria spp. and Listeria monocytogenes in ready-to-eat foods with various selective culture media. Food Control. 32: 19–24.
Karpiskova R., S. Pejchalova, J. Mokroova, J. Vytrasova, P. Smuharova and J. Ruprich. 2000. Application of a chromogenic medium and the PCR method for the rapid confirmation of Listeria monocytogenes in foodstuffs. J. Microbiol. Methods. 41: 267–271.
Kawasaki S., N. Horikoshi, Y. Okada, K. Takeshita, T. Sameshima and S. Kawamoto. 2005. Multiplex PCR for simultaneous detection of Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7 in meat samples. J. Food. Prot. 68: 551–556.
Kuan C.H., S.G. Goh, Y.Y. Loo, W.S. Chang, Y.L Lye, S. Puspanadan, J.Y.H. Tang, Y. Nakaguchi, M. Nishibuchi, N.A. Mahyudin and others. 2013. Prevalence and quantification of Listeria mono-cytogenes in chicken offal at the retail level in Malaysia. Poult. Sci. 92: 1664–1669.
Lawrence L.M. and A. Gilmour. 1994. Incidence of Listeria spp. and Listeria monocytogenes in a poultry processing environment and in poultry products and their rapid confirmation by multiplex PCR. Appl. Environ. Microbiol. 60: 4600–4604.
Marian M.N., S.M. Aminah, M.I. Zuraini, R. Son, M. Maimunah, H.Y. Lee, W.C. Wong and N. Elexson. 2012. MPN-PCR detection and antimicrobial resistance of Listeria monocytogenes isolated from raw and ready-to-eat foods in Malaysia. Food Control. 28: 309–314.
Peres N.D., C.C. Lange, M.A.V.P. Brito, J. R. F. Brito, E.F. Arcuri and M.M.O.P. Cerqueira. 2010. Detecção de Listeria monocytogenes pela técnica de PCR em leite contaminado artificialmente. Arq.Bas. Bet. Zootec. 62: 973–979.
Van Coillie E., H. Werbrouck, M. Heyndrickx, L. Herman and N. Rijpens. 2004. Prevalence and typing of Listeria monocytogenes in ready-to-eat food products on the Belgian market. J. Food. Prot. 67: 2480–2487.
Xiaoyun Q., L. Wu, H. Huang, P.E. McDonel, A.V. Palumbo, J.M. Tiedje and J. Zhou. 2001. Evaluation of PCR-generated chimeras, mutations, and geterouplex with 16s rRNA Gene-Based Cloning. Appl. Environ. Microbiol. 67: 880–887