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Citation Information : Polish Journal of Microbiology. Volume 66, Issue 2, Pages 255-257, DOI: https://doi.org/10.5604/01.3001.0010.7869
License : (CC BY-NC-ND 4.0)
Received Date : 05-August-2016 / Accepted: 12-January-2017 / Published Online: 28-June-2017
Several studies have observed that use of a conventional PCR protocol with primers LM1 and LM2 for the identification of the hlyA gene of Listeria monocytogenes generates non-specific PCR amplifications and false positives. For this reason, in this study we provide a modified PCR protocol that improves the specificity of the results obtained with LM1 and LM2 primers.
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