An Improve Protocol for PCR Using LM1 and LM2 Primers for Listeria monocytogenes Detection in Food Matrices

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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology

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ISSN: 1733-1331
eISSN: 2544-4646

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VOLUME 66 , ISSUE 2 (June 2017) > List of articles

An Improve Protocol for PCR Using LM1 and LM2 Primers for Listeria monocytogenes Detection in Food Matrices

Angélica Godínez-Oviede / Gerardo M. Nava / Sofía M. Arvizu-Medrano / Montserrat Hernández-Iturriaga *

Keywords : Listeria monocytogenes, hlyA, PCR

Citation Information : Polish Journal of Microbiology. VOLUME 66 , ISSUE 2 , ISSN (Online) 2544-4646, DOI: 10.5604/01.3001.0010.7869, June 2017

License : (CC BY-NC-ND 4.0)

Received Date : 05-August-2016 / Accepted: 12-January-2017 / Published Online: 28-June-2017

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ABSTRACT

Several studies have observed that use of a conventional PCR protocol with primers LM1 and LM2 for the identification of the hlyA gene of Listeria monocytogenes generates non-specific PCR amplifications and false positives. For this reason, in this study we provide a modified PCR protocol that improves the specificity of the results obtained with LM1 and LM2 primers.

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