Bactericidal Activity of Octenidine to Various Genospecies of Borrelia burgdorferi, Sensu Lato Spirochetes in Vitro and in Vivo

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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology

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ISSN: 1733-1331
eISSN: 2544-4646

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VOLUME 66 , ISSUE 2 (June 2017) > List of articles

Bactericidal Activity of Octenidine to Various Genospecies of Borrelia burgdorferi, Sensu Lato Spirochetes in Vitro and in Vivo

Stanisława Tylewska-Wierzbanowska / Urszula Roguska / Grażyna Lewandowska / Tomasz Chmielewski *

Keywords : Borrelia burgdorferi, octenidine hydrochloride, bactericidal activity

Citation Information : Polish Journal of Microbiology. VOLUME 66 , ISSUE 2 , ISSN (Online) 2544-4646, DOI: 10.5604/01.3001.0010.7874, June 2017

License : (CC BY-NC 4.0)

Received Date : 11-August-2016 / Accepted: 27-January-2017 / Published Online: 28-June-2017

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ABSTRACT

The aim of our studies was to invent a reliable method for detection of the bactericidal activity of disinfectants against Borrelia burgdorferi in suspension (in vitro) and in cell line cultures (in vivo). In the suspension method, 0.01% octenidine at 20°C and 35°C was bactericidalto Borrelia afzeli; Borrelia garini, B. burgdorferi sensu stricto after 5 minutes treatment. Increase of the temperature to 35°C speed up the bactericidal effect to 1 minute. The bactericidal action of octenidine towards B. burgdorferi spirochetes growing in fibroblasts was less effective and needed a longer time to kill them than in the suspension.

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