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Polish Journal of Microbiology
Polish Society of Microbiologists
Subject: Microbiology
ISSN: 1733-1331
eISSN: 2544-4646
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Betaine Improves Polymer-Grade D-Lactic Acid Production by Sporolactobacillus inulinus Using Ammonia as Green Neutralizer
Guoping Lv / Chengchuan Che / Li Li / Shujing Xu / Wanyi Guan / Baohua Zhao / Jiansong Ju *
Keywords : Sporolactobacillus inulinus, ammonia as neutralizer, betaine as osmoprotectant, D-lactic acid production
Citation Information : Polish Journal of Microbiology. VOLUME 66 , ISSUE 2 , ISSN (Online) 2544-4646, DOI: 10.5604/01.3001.0010.7880, June 2017
License : (CC BY-NC-ND 4.0)
Received Date : 20-April-2016 / Accepted: 08-September-2016 / Published Online: 28-June-2017
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ARTICLE
ABSTRACT
The traditional CaCO3-based fermentation process generates huge amount of insoluble waste. To solve this problem, we have developed an efficient and green D-lactic acid fermentation process by using ammonia as neutralizer. The 106.7 g/l of D-lactic acid production and 0.89 g/g of consumed sugar were obtained by Sporolactobacillus inulinus CASD with a high optical purity of 99.7% by adding 100 mg/l betaine in the simple batch fermentation. The addition of betaine was experimentally proven to protect cells at high concentration of ammonium ion, increase D-lactate dehydrogenase specific activity and thus promote the production of D-lactic acid.
FIGURES & TABLES
REFERENCES
Bradford M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.
Ding S. and T. Tan. 2006. L-lactic acid production by Lactobacillus casei fermentation using different fed-batch feeding strategies. Process. Biochem. 41: 1451–1454.
Ikada Y., K. Jamshidi, H. Tsuji and S.H. Hyon. 1987. Stereocomplex formation between enantiomeric poly (lactides). Macromolecules. 20: 904–906.
Li Y., L. Wang, J. Ju, B. Yu and Y. Ma. 2013. Efficient production of polymer-grade D-lactate by Sporolactobacillus laevolacticus DSM442 with agricultural waste cottonseed as the sole nitrogen source. Bioresour. Technol. 142: 186–191.
Miura S., L. Dwiart, T. Arimur, M. Hoshino, L. Tiejun and M. Okabe. 2004. Enhanced production of L-lactic acid by ammonia-tolerant mutant strain Rhizopus sp. MK-96-1196. J. Biosci. Bioeng. 97: 19–23.
Okano K., Q. Zhang, S. Shinkawa, T. Tanaka, H. Fukuda and A. Kondo. 2009. Efficient production of optically pure D-lactic acid from raw corn starch by using a genetically modified L-lactate dehy-drogenase gene-deficient and alpha-amylase-secreting Lactobacillus plantarum strain. Appl. Environ. Microbiol. 75: 462–467.
Robert H., C. Le Marrec, C. Blanco and M. Jebbar. 2000. Glycine betaine, carnitine, and choline enhance salinity tolerance and prevent the accumulation of sodium to a level inhibiting growth of Tetragenococcus halophila. Appl. Environ. Microbiol. 66: 509–517.
Sharma P. and R.S. Dubey. 2005. Modulation of nitrate reductase activity in rice seedlings under aluminium toxicity and water stress: role of osmolytes as enzyme protectant. J. Plant Physiol. 162: 854–864.
Vaidya A.N., R.A. Pandey, S. Mudliar, M. Suresh Kumar, T. Chakrabarti and S. Devotta. 2005. Production and recovery of lactic acid for polylactide-an overview. Crit. Rev. Environ. Sci. Technol. 35: 429–467.
Wang L., B. Zhao, F. Li, K. Xu, C. Ma, F. Tao, Q. Li and P. Xu. 2011. Highly efficient production of D-lactate by Sporolactobacillus sp. CASD with simultaneous enzymatic hydrolysis of peanut meal. Appl. Microbiol. Biotechnol. 89: 1009–1017.
Wang Y., T. Tian, J. Zhao, J. Wang, T. Yan, L. Xu, Z. Liu, E. Garza, A. Iverson, R. Manow and others. 2012. Homofermentative production of D-lactic acid from sucrose by a metabolically engineered Escherichia coli. Biotechnol. Lett. 34: 2069–2075.
Zhang Y. and P.V. Vadlani. 2013. D-Lactic acid biosynthesis from biomass-derived sugars via Lactobacillus delbrueckii fermentation. Bioproc. Biosyst. Eng. 36: 1897–1904.