Betaine Improves Polymer-Grade D-Lactic Acid Production by Sporolactobacillus inulinus Using Ammonia as Green Neutralizer


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Polish Journal of Microbiology

Polish Society of Microbiologists

Subject: Microbiology


ISSN: 1733-1331
eISSN: 2544-4646





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VOLUME 66 , ISSUE 2 (June 2017) > List of articles

Betaine Improves Polymer-Grade D-Lactic Acid Production by Sporolactobacillus inulinus Using Ammonia as Green Neutralizer

Guoping Lv / Chengchuan Che / Li Li / Shujing Xu / Wanyi Guan / Baohua Zhao / Jiansong Ju *

Keywords : Sporolactobacillus inulinus, ammonia as neutralizer, betaine as osmoprotectant, D-lactic acid production

Citation Information : Polish Journal of Microbiology. Volume 66, Issue 2, Pages 273-276, DOI:

License : (CC BY-NC-ND 4.0)

Received Date : 20-April-2016 / Accepted: 08-September-2016 / Published Online: 28-June-2017



The traditional CaCO3-based fermentation process generates huge amount of insoluble waste. To solve this problem, we have developed an efficient and green D-lactic acid fermentation process by using ammonia as neutralizer. The 106.7 g/l of D-lactic acid production and 0.89 g/g of consumed sugar were obtained by Sporolactobacillus inulinus CASD with a high optical purity of 99.7% by adding 100 mg/l betaine in the simple batch fermentation. The addition of betaine was experimentally proven to protect cells at high concentration of ammonium ion, increase D-lactate dehydrogenase specific activity and thus promote the production of D-lactic acid.

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