Publications
Polish Journal of Microbiology
Polish Society of Microbiologists
Subject: Microbiology
ISSN: 1733-1331
eISSN: 2544-4646
SEARCH WITHIN CONTENT
Assessment of the Microbiological Status of Probiotic Products
Anna Zawistowska-Rojek * / Tomasz Zaręba / Agnieszka Mrówka / Stefan Tyski
Keywords : antimicrobial resistance, dietary supplement, FSMP, medicinal product, probiotics
Citation Information : Polish Journal of Microbiology. Volume 65, Issue 1, Pages 97-104, DOI: https://doi.org/10.5604/17331331.1197281
License : (CC BY-NC-ND 4.0)
Received Date : 02-February-2015 / Accepted: 11-August-2015 / Published Online: 15-March-2016
- ARTICLE
- FIGURES & TABLES
- REFERENCES
- EXTRA FILES
- COMMENTS
ARTICLE
ABSTRACT
The aim of this study was to perform the microbiological analysis of quality of 25 probiotic products, available on the Polish market. Analysis of bacterial viability in probiotic products showed that not all of these preparations possess a suitable number of bacteria. Moreover, some of the tested probiotic products contained bacterial strains other than those declared by the manufacturer. All tested strains recovered from probiotic products were found to be resistant to metronidazole and susceptible to nitrofurantoin. The susceptibility to other antibiotics was strain specific. Probiotic products should be subject to regular and thorough inspection by appropriate institutions.
FIGURES & TABLES
REFERENCES
Ashraf R. and N.P. Shah. 2011. Antibiotic resistance of probiotic organisms and safety of probiotic dairy products. Int. J. Food Micro-biol. 18(3): 837–853.
Chapman C.M.C., G.R. Gibson and I. Rowland. 2011. Health benefits of probiotics: are mixtures more effective than single strains? Eur. J. Nutr. 50: 1–17.
Clinical and Laboratory Standards Institute. 2011. Performance standards for antimicrobial susceptibility testing; twenty-first infor-mation supplement. Document M100-S21. Wayne, PA: CLSI.
Coeuret V., M. Gueguen and J.P. Vernoux. 2004. Numbers and strains of lactobacilli in some probiotic products. Int. J. Food Microbiol. 97: 147–156.
Czinn S.J. and S.S. Blanchard. 2009. Probiotics in foods and supplements, pp. 299–306. In: Michail S. and P.M. Sherman (eds). Nutrition and health: Probiotics in Pediatric Medicine. Totowa: Humana Press.
Danielsen M. and A. Wind. 2003. Susceptibility of Lactobacillus spp. to antimicrobial agents. Int. J. Food Microbiol. 82: 1–11.
European Committee on Antimicrobial Susceptibility Testing. 2012. EUCAST Disk Diffusion Method for Antimicrobial Susceptibility Testing. www.eucast.org. January, 2012
Gueimonde M., B. Sanchez, C.G. Reyes-Gavilan and A. Margolles. 2013. Antibiotic resistance in probiotic bacteria. Front Microbiol. 4: 1–6.
Hamilton-Miller J.M.T., S. Shah and J.T. Winkler. 1998. Public health issues arising from microbiological and labeling quality of foods and supplements containing probiotic microorganisms. Public Health Nutr. 2(2): 223–229.
Holzapfel W.H., P. Haberer, R. Geisen, J. Björkroth and U. Schillinger. 2001. Taxonomy and important features of probiotic microorganisms in food and nutrition. Am. J. Clin. Nutr. 73(suppl): 365S–73S.
Hummel A.S., C. Hertel, W.H. Holzapfel and C.M. Franz. 2007. Antibiotic resistances of starter and probiotic strains of lactic acid bacteria. Appl. Environ. Microbiol. 73: 730–739.
Liu C., Z.Y. Zhang, K. Dong, J.P. Yuan and X.K. Guo. 2009. Antibiotic resistance of probiotic strains of lactic acid bacteria isolated from marketed foods and drugs. Biomed. Environ. Sci. 22: 401–412.
Nawaz M., J. Wang, A. Zhou, C. Ma, X. Wu, J.E. Moore, B.C. Millar and J. Xu. 2011. Characterization and transfer of antibiotic resis-tance in lactic acid bacteria from fermented food products. Curr. Microbiol. 62: 1081–1089.
Pereira D.I.A., A.L. McCartney and G.R. Gibson. 2003. An in vitro study of the probiotic potential of a bile-salt-hydrolyzing Lactobacil-lus fermentum strain, and determination of its cholesterol-lowering properties. Appl. Environ. Microbiol. 69(8): 4743–4752.
Report FAO/WHO. 2001. Probiotics in food: Health and nutritional properties and guidelines for evaluation, pp. 1–29. ftp://ftp.fao.org/docrep/fao/009/a0512e/a0512e00.pdf, 2015.02.02.
Report FAO/WHO. 2002. Guidelines for the Evaluation of Probiotics in Food, p. 36–48. http://www.who.int/foodsafety/fs_manage-ment/en/probiotic_guidelines.pdf, 2015.02.02.
Sanders M.E. and J. Huis in’t Veld. 1999. Bringing a probiotic-containing functional food to the market: microbiological, product, regulatory and labeling issues. Antonie Van Leeuwenhoek 76: 293–315.
Szajewska H., A. Fordymacka and A. Banaszkiewicz. 2002. Microbiological qualitative and quantitative analysis of probiotic products registered in Poland for medicinal purposes (pilot study) (in Polish). Pediatria Polska LXXVII: 941–944.
Szajewska H., A. Fordymacka, J. Bardowski, R.K. Górecki,J.Z. Mrukowicz and A. Banaszkiewicz. 2004. Microbiological and genetic analysis of probiotic products licensed for medicinal purposes. Med. Sci. Monit 10(9): BR346–350.
Temmerman R., B. Pot, G. Huys and J. Swings. 2002. Identification and antibiotic susceptibility of bacteria isolates from probiotic products. Int. J. Food Microbiol. 81: 1–10.
Wiatrzyk A., U. Czajka and B. Bucholc. 2007. Resistance to antibiotics of Lactobacillus and Bifidobacterium strains present in probiotics preparations (in Polish). Pediatria Polska 82: 12.
Wiatrzyk A., M. Polak, U. Czajka, K. Krysztopa-Grzybowska and A. Lutyńska. 2013. Phenotypic and genotypic characterization of probiotic bacterial strains used in medicinal products (in Polish) Med. Dośw. Mikrobiol. 65: 47–56.